Hyperglycaemia-induced fibrotic and inflammatory gene expression alterations in lung epithelial cells: Implications for pulmonary fibrosis development

Article

Author: Bhardwaj, Priya ; Maruthi, Mulaka

Hyperglycaemia has a significant long-term impact on multiple organ systems, including renal, cardiovascular, central nervous, hepatic and ocular systems, leading to the gradual loss of their functional abilities. Numerous studies have elucidated the pathophysiology, etiology, and consequences of hyperglycaemia on these organs. The pulmonary system is also considered as a target of hyperglycaemia, several factors cause lung injury which leads to the development of pulmonary fibrosis, a chronic fibrotic disease with usual interstitial pneumonia patterns. Nevertheless, the effects of hyperglycaemia on the development of pulmonary fibrosis remain poorly understood. We intend to understand the cellular and morphological changes, and the progression of fibrosis in lung epithelial cells subjected to hyperglycaemia. Our experimental data indicate that hyperglycaemia induces fibrotic and inflammatory alterations in cultured lung epithelial cells. These alterations are facilitated by the upregulation of genes related to fibrosis and inflammation, promoting cell proliferation and migration. Further research is required to comprehensively elucidate the impact of hyperglycaemia during lung injury progression of fibrosis, these findings may reveal novel mechanisms that may help in the assessment and treatment of lung ailments in people with hyperglycaemia.

01 Jul 2025·Plant Physiology and Biochemistry

Genomic introgression and expression profiling of the KTI null allele in soybean through elite-by-elite backcrossing

Article

Author: Mahobia, Arpita ; Kokane, Sunil ; VineetKumar ; Mane, Shyamsunder ; Jadhav, Pravin ; Deshmukh, Rupesh ; Gawande, Bhagyashree ; Shinde, Umesh ; Kharche, Vilas ; Moharil, Mangesh P ; Sonah, Humira ; Nichal, Satish S ; Dani, Raviprakash ; Rani, Anita ; Thakur, Pranita

Soybean stands out with a high protein (35-45 %) and oil content (18-22 %) is one of the important plant-based protein sources. However, the presence of Kunitz Trypsin Inhibitor (KTI), an anti-nutritional factor, reduces the bioavailability of soybean proteins. While thermal inactivation of KTI is possible, it compromises protein solubility and leaves residual activity. Therefore, eliminating KTI through genetic approaches is crucial to improving soybean's nutritional profile. This study aimed to develop soybean genotypes devoid of KTI through marker-assisted selection (MAS) to enhance their nutritional value and global marketability. Hybridization was conducted between recipient parents (AMS-MB-5-18 and AMS-MB-5-19) and donor parents (NRC-101 and NRC-127) to produce four cross combinations. Marker-assisted screening was used to identify plants carrying the null KTI allele across successive backcross generations (BC₁F₁ to BC₁F₄). A total of 342 SSR markers were used to assess molecular polymorphism and determine recurrent parent genome content (RPGC) recovery. Absence for KTI peptide was confirmed with native polyacrylamide gel electrophoresis (PAGE). In addition, expression of the KTI3 gene was evaluated during seed developmental stages (R5 and R6) using quantitative PCR. Hybridization efforts yielded 60 F1 seeds with a 12 % success rate. Backcrossing resulted in the production and screening of 259 BC₁F₂ plants, 45 BC₁F₃ plants, and 8 BC₁F₄ plants carrying the null KTI allele. RPGC recovery increased across generations, with BC₁F₄ plants achieving 84.15 % in Cross A (AMS MB 5-18 X NRC 101) and 83.72 % in Cross B (AMS MB 5-18 X NRC 127). Native PAGE confirmed the absence of KTI peptide in 8 BC₁F₄ plants. Expression profiling of KTI3 gene via qRT-PCR revealed that expression was significantly higher during the R5 stage compared to R6, with fold changes ranging from 1.39 to 267.64 in R5 and 8.19 to 130.77 in R6. Null parent lines exhibited minimal KTI3 expression, consistent with their genetic background. These findings confirmed the successful introgression of the null KTI allele and its desired effect on trait in advanced backcross populations.

01 Jul 2025·Inorganic Chemistry Communications

Zinc(II) Complexes: Synthetic and antiviral Perspective

Author: Richa ; Kataria, Ramesh ; Jyoti ; Choudhary, Manshu ; Kumar, Vinod ; Yadav, Pragati ; Das, Arpan ; Kumari, Pratima ; Kumar, Aman

Zinc deficiency causes serious health effects as zinc is essential in many physiol. functions.Its deficiency weakens the immune system, which raises the risk of infection and disease-related death and is linked to many prevalent disorders.The primary focus of this review is associated with the synthesis and antiviral properties of Zn(II) complexes using different kinds of important ligands.It covers the literature from 1993 onwards.Zinc(II) complexes as antiviral agents have been proven to be efficacious towards a variety of viruses such as influenza virus, herpes simplex virus, coronavirus, vaccinia virus, human adenovirus, vesicular stomatitis virus, HIV, and SARS-CoV-2.

100 Deals associated with Central University of Haryana

100 Translational Medicine associated with Central University of Haryana

Corporation Tree

Boost your research with our corporation tree data.

view full example data

Pipeline

Pipeline Snapshot as of 01 Aug 2025

No data posted

Deal

Boost your decision using our deal data.

view full example data

Translational Medicine

Boost your research with our translational medicine data.

view full example data

Profit

Explore the financial positions of over 360K organizations with Synapse.

view full example data

Grant & Funding(NIH)

Access more than 2 million grant and funding information to elevate your research journey.

view full example data

Investment

Gain insights on the latest company investments from start-ups to established corporations.

view full example data

Financing

Unearth financing trends to validate and advance investment opportunities.

view full example data

AI Agents Built for Biopharma Breakthroughs

Accelerate discovery. Empower decisions. Transform outcomes.

Get started for free today!

Accelerate Strategic R&D decision making with Synapse, PatSnap’s AI-powered Connected Innovation Intelligence Platform Built for Life Sciences Professionals.

Start your data trial now!

Synapse data is also accessible to external entities via APIs or data packages. Empower better decisions with the latest in pharmaceutical intelligence.

Bio

Bio Sequences Search & Analysis

Chemical

Chemical Structures Search & Analysis

Central University of Haryana

Overview

Related

Corporation Tree

Pipeline

Pipeline Snapshot as of 01 Aug 2025

Deal

Translational Medicine

Profit

Grant & Funding(NIH)

Investment

Financing