Inhibition of Ultraviolet B-induced Apoptosis in Fibroblasts by Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Media via the Phosphatidylinositol-3-Kinase/Akt Pathway.

Article

Author: Chouw, Angliana ; Dewi, Dian Andriani Ratna ; Pangkahila, Wimpie ; Dewi, Nurrani Mustika ; Sandra, Ferry ; Astawa, I Nyoman Mantik ; Celinna, Maria

BackgroundUltraviolet B (UVB) irradiation induces photoaging and apoptosis in various cell types. Inhibition of UVB-induced apoptotic pathways has been explored in different apoptotic cascades. Conditioned media from human umbilical cord blood mesenchymal stem cells (CM-hUCB-MSC) contain important substances for cell regeneration. However, the potential of CM-hUCB-MSC in preventing UVB-induced apoptosis has not been clearly elucidated. Therefore, the current research was conducted to investigate the potential of CM-hUCB-MSC in inhibiting UVB-induced apoptosis and its role in the antiapoptotic signaling pathway.MethodsAn experimental in vitro study was conducted at PT. Prodia StemCell Indonesia, Jakarta, Indonesia, 2019-2022. Initially, hUCB-MSCs were isolated and cultured to produce CM-hUCB-MSC. NIH3T3 cells were pretreated with/without 50 μM LY294002, treated with/without 10% CM-hUCB-MSC, and then irradiated with/without UVB. Subsequently, the cells were analyzed using sub-G1, immunofluorescence, and immunoblotting assays. One-way analysis of variance (ANOVA) was used for data analysis, followed by Tukey's honest significant difference (HSD) test or the Kruskal-Wallis test, followed by the Dunn-Bonferroni test using IBM SPSS Statistics software version 21. Statistical significance was determined at P<0.05.ResultsCM-hUCB-MSC significantly inhibited UVB-induced apoptosis in NIH3T3 cells (P=0.002, Dunn-Bonferroni test). CM-hUCB-MSC significantly induced Akt phosphorylation at Ser 473 in UVB-irradiated NIH3T3 cells (P<0.001, Tukey's HSD test). The CM-hUCB-MSC-induced phosphorylation of Akt was significantly inhibited by LY294002 (P<0.001, Tukey's HSD test).ConclusionTaken together, it can be concluded that CM-hUCB-MSC inhibits UVB-induced NIH3T3 cell apoptosis via the activation of phosphatidylinositol-3-kinase (PI3K)/Akt signaling cascades.

01 Jan 2020·Journal of Advanced Pharmaceutical Technology & Research

Epidermal growth factor in sacran hydrogel film accelerates fibroblast migration

Article

Author: Pratama, Arvenda Rezky ; Wathoni, Nasrul ; Mohammed, Ahmed Fouad Abdelwahab ; Arima, Hidetoshi ; Okajima, Maiko ; Rusdiana, Taofik ; Putera, Bayu Winata ; Hasanah, Aliya Nur ; Kaneko, Tatsuo

Epidermal growth factor (EGF) accelerates epidermal regeneration, and it is widely studied as a wound-healing agent. However, the special carrier for the topical administration of EGF is urgently needed to deliver EGF on the wound site. In a preceding study, sacran hydrogel film (Sac-HF) showed a possible use as a dressing material for wound healing, as well as a good capability as a drug carrier. In the current study, we prepared Sac-HF containing EGF (Sac/EGF-HF) and then characterized their physicochemical properties, including thickness, swelling ratio, degradability, tensile strength, and morphology. In addition, we have also conducted thermal and crystallography studies using differential scanning calorimetry (DSC) and X-ray diffraction, respectively. Furthermore, we investigated the in vitro influence of Sac/EGF-HF on cell migration using a fibroblast cell line. Morphology study confirmed that the casting method used for the film preparation resulted in a homogeneous film of Sac/EGF-HF. Furthermore, EGF significantly increased the thickness, tensile strength, and degradability of Sac/EGF-HF compared to Sac-HF. Sac/EGF-HF had a lower swelling ability compared to Sac-HF; this result corroborated the tensile strength result. Interestingly, X-ray diffraction and DSC results showed that Sac/EGF-HF had an amorphous shape. The in vitro studies revealed that Sac/EGF-HF induced the fibroblast migration activity. These results conclude that Sac/EGF-HF has the potential properties of HF for biomedical applications.

Brazilian Archives of Biology and Technology

Histone Potentiates Inositol Hexakisphosphate in Inducing Apoptosis of HONE-1 Nasopharyngeal Cancer Cells

Author: Celinna, Maria ; Annisa, Sheila ; Lee, Kyung Hoon ; Rahayu, Wiryadani ; Anthony, Ian ; Sandra, Ferry ; Chouw, Angliana ; Lokantari, Miranti Anggorodhiyu

Abstract Inositol hexakisphosphate (InsP6) requires relatively high concentrations to induce apoptosis of cancer cells, which can possibly cause apoptosis of normal cells. Anticancer ability of InsP6 could be preserved by combining with histone, so InsP6 can be used at low concentrations. The effect of InsP6 and histone combination has not been investigated on nasopharyngeal cancer cells. The current study elucidated the effect of InsP6 and its combination with histone on the apoptosis of HONE-1 cells. The most effective concentration and the cellular mechanisms by which this combination exerts its anticancer effects were also investigated. HONE-1 and NIH3T3 cells (as normal control cells) were treated with InsP6 and/or histone in different concentrations. Apoptosis percentages of the treated cells were measured with sub-G1 assay. Nuclear fragmentation and mitochondrial membrane potential (∆Ψm) reduction in the treated HONE-1 cells were confirmed with 4',6-diamidino-2-phenylindole (DAPI) staining and ∆Ψm assay, respectively. The combination of 10 μM InsP6 and 10 μg/mL histone had the optimal ability to induce the apoptosis of HONE-1 cells. This combination did not induce apoptosis of NIH3T3 cells. The apoptosis-inducing ability of this combination was higher than that of 10 μM InsP6 merely. The ability of InsP6 to induce apoptosis of HONE-1 cells could be enhanced by histone application. Combination of 10 μM InsP6 and 10 μg/mL histone might be the optimal concentrations for inducing apoptosis in HONE-1 cells.

100 Deals associated with PT Prodia Stemcell Indonesia

100 Translational Medicine associated with PT Prodia Stemcell Indonesia

Corporation Tree

Boost your research with our corporation tree data.

view full example data

Pipeline

Pipeline Snapshot as of 11 May 2025

No data posted

Deal

Boost your decision using our deal data.

view full example data

Translational Medicine

Boost your research with our translational medicine data.

view full example data

Profit

Explore the financial positions of over 360K organizations with Synapse.

view full example data

Grant & Funding(NIH)

Access more than 2 million grant and funding information to elevate your research journey.

view full example data

Investment

Gain insights on the latest company investments from start-ups to established corporations.

view full example data

Financing

Unearth financing trends to validate and advance investment opportunities.

view full example data

Chat with Hiro

Get started for free today!

Accelerate Strategic R&D decision making with Synapse, PatSnap’s AI-powered Connected Innovation Intelligence Platform Built for Life Sciences Professionals.

Start your data trial now!

Synapse data is also accessible to external entities via APIs or data packages. Empower better decisions with the latest in pharmaceutical intelligence.

Bio

Bio Sequences Search & Analysis

Chemical

Chemical Structures Search & Analysis

PT Prodia Stemcell Indonesia

Overview

Related

Corporation Tree

Pipeline

Pipeline Snapshot as of 11 May 2025

Deal

Translational Medicine

Profit

Grant & Funding(NIH)

Investment

Financing