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The use of a new product, Microcon/Micropure (a centrifugal ultrafiltration device combined with a microporus insert), for the purification of DNA, RNA, peptides and proteins from gels is described. Using this system, DNA can be recovered from agarose gel in concentrated, contamination-free form in only 15 min. Results of studies on the effects of fragment size and various pretreatment of the gel slice on DNA recovery are presented. The Microcan/Micropure combination can also be used for the recovery of macromolecules from polyacrylamide gels. Optimized protocols for the recovery of RNA, oligonucleotides and proteins from polyacrylamide gels using a crush and elute method, along with a study of critical parameters, are presented.

01 Nov 1993·American biotechnology laboratory

Ultrafiltration in molecular biology.

Article

Author: Schratter, P ; Krowczynska, A M ; Leonard, J T

In conclusion, the availability of ultrafiltration devices offers the molecular biologist a choice of tools for a wide variety of applications. They provide means for easy and fast processing of nucleic acid and protein samples, with high yield and conservation of valuable biological material.

01 Feb 1993·Nature Biotechnology

Virus Harvesting and Affinity-Based Liquid Chromatography

Review

Author: Balkovic, E S ; O'Neil, P F

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