In view of the disadvantages of food exudate juice during storage, a bionic superamphiphobic (SA) film was prepared. Different hydrophobic and oleophobic films were prepared by tape casting method with waterborne polyurethane (WPU) as film-forming matrix, diatomite (DME) as roughness construction factor, 1H,1H,2H,2H-perfluorooctane trichlorosilane (PFOTS) as low surface energy modifier, and eugenol (EG) as active antibacterial agent. The results show that the SA-EG-DME/WPU film has excellent superamphiphobic properties (WCA > 150°, OCA>120°). The film has secondary slow-release microstructures, and the embedding rate of eugenol is 82.03 %, which can play antibacterial role in a long time. After the composite film treatment, Shewanella putrefaciens collapses and shrinks, the cell membrane permeability increases, and the osmotic pressure is unbalanced. The WPU film exhibits excellent preservation properties for Salmon fillets due to the isolation juice property combined with the slow-release property of the preservative. The superamphiphobic film prepared in this study has potential application value in the field of food preservation.