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01 Aug 1997·Applied and Environmental MicrobiologyQ2 · BIOLOGY

Modified quantitative association of official analytical chemists sporicidal test for liquid chemical germicides

Q2 · BIOLOGY

Article

Author: Maida, Braulia ; Carr, Cheri D. ; Schlotfeld, Lindy ; Armstrong, Michelle ; Miner, Norman

The Association of Official Analytical Chemists (AOAC) test for sporicidal activity of disinfectants (966.04) is used in the United States as the legal criteria for classifying a liquid as a chemical sterilant and also as the indicator of the highest level of disinfectant. This qualitative test contains procedures that may cause inaccurate results. A modified, quantitative version of the AOAC sporicidal test has been developed that uses a specified minimum number of spores (2.0 x 10(sup5)) of Bacillus subtilis or Clostridium sporogenes dried onto porcelain penicylinders. This modified test was validated with three commercial sterilant chemicals tested on three separate groups of spore-labeled cylinders.

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