/ Unknown statusPhase 2IIT A Multicenter, Randomized, Double-blind, Placebo-controlled Clinical Study on Specific-Population to Evaluate the Safety and Efficacy of Therapeutic Double-plasmid HBV DNA Vaccine in HBeAg-positive Patients With Chronic Hepatitis B
To preliminarily evaluate the efficiency and safety of therapeutic double- plasmid HBV DNA vaccine on HBeAg-positive, chronic hepatitis B patients, and provide evidence for the next dosing regimen.
100 Clinical Results associated with Guangzhou Baidi Bio-Technology Co., Ltd.
0 Patents (Medical) associated with Guangzhou Baidi Bio-Technology Co., Ltd.
Guangdong Yaoxueyuan Xuebao
Cloning and expression of human prothymosin α and interleukin-2 fusion gene in E.coli
Author: Zhou, Feiyan ; Wei, Jian ; Huang, Ming
Objective: To clone human prothymosin and interleukin-2 fusion gene into the prokaryotic expression vector and to express the fusion protein effectively in E. coli system.Methods: The human prothymosin and interleukin-2 gene were amplified by RT-PCR from human leukemia cells and T lymphocytes resp.The two genes were fused with a linker and the fusion gene fragment was used to construct a prokaryotic expression vector pET42a-PI by DNA recombinant technique.After identification by restriction anal. and DNA sequencing, the recombinant plasmid was transformed into E. coli Rosetta.The overexpressed protein induced by IPTG was purified by anion exchange chromatog. and Sephacryl S-200 chromatog.The biol. activity of the fusion protein was assayed using T cell E-rosette formation test of human blood.Results: The PCR product was about 750 bp in length, which was consistent with the expected size of the fusion gene.Plasmid pET42a-PI was transformed into Rosetta and a new protein with a relative mol. weight of 28 kDa was expressed.The purified fusion protein could increase the E-rosette formation rate of human peripheral mononuclear cells.Conclusion: The encoding sequence of human prothymosin and interleukin-2 fusion gene was successfully cloned into the prokaryotical expression vector pET42a and can be expressed effectively in E. coli system.
100 Deals associated with Guangzhou Baidi Bio-Technology Co., Ltd.
100 Translational Medicine associated with Guangzhou Baidi Bio-Technology Co., Ltd.