Delving into the Latest Updates on Shaanxi Jiuzhou Biotechnology Co. Ltd. with Synapse

Overview

Protein refolding is a key process for the production of recombinant proteins from E. coli.One of the effective techniques for protein refolding is through liquid chromatog. named as protein folding liquid chromatog. (PFLC).Mobile phase composition is one of the most important factors affecting the efficiency of protein renaturation in PFLC.Optimization of mobile phase composition in PFLC for protein renaturation with simultaneous purification is thus much more important than that in usual liquid chromatog.This study presents an approach with regard to the increase of protein production through optimizing the mobile phase composition of PFLC.Recombinant human interferon-γ (rhIFN-γ) was purified with simultaneous renaturation by high performance hydrophobic interaction chromatog. (HPH-IC) using a stationary phase with an end group of PEG-200.Effects of mobile phase composition, gradient elution mode, and flow rate on the recoveries of mass and bioactivity of rhIFN-γ were investigated.It was found that these factors were very important for refolding with simultaneous purification of rhIFN-γ by PFLC.This study highlights the importance of optimizing the mobile phase composition in PFLC for increasing the recovery of protein.Taking 3.0 mol/L (NH₄)₂SO₄ +0.05 mol/L KH₂PO₄ (pH 7.0) and 0.05 mol/L KH₂PO₄ (pH 7.0) as mobile phases A and B, resp., the highest mass recovery was obtained under a nonlinear gradient elution for 35 min.

100 Deals associated with Shaanxi Jiuzhou Biotechnology Co. Ltd.

Login to view more data

100 Translational Medicine associated with Shaanxi Jiuzhou Biotechnology Co. Ltd.

Login to view more data

Corporation Tree

Boost your research with our corporation tree data.

login

or

view full example data

Boost your research with our corporation tree data.

Pipeline

Pipeline Snapshot as of 15 Jun 2025

The statistics for drugs in the Pipeline is the current organization and its subsidiaries are counted as organizations,Early Phase 1 is incorporated into Phase 1, Phase 1/2 is incorporated into phase 2, and phase 2/3 is incorporated into phase 3

Other

1

Login to view more data