This paper aims to establish determination method of furaltadone metabolites antibiotic residue in milk with magnetic particle chemiluminescence. To label furaltadone metabolite, ovalbumin was conjugated on the magnetic particles, and the luminous matter iso-luminol was labeled on furaltadone metabolite-furaltadone metabolite monoclonal antibody, by establishing a standard curve of furaltadone metabolite to quant. detect furaltadone metabolite. 60 Copies of milk were determined by established methods, to compare it with enzyme-linked immunoassay reagent kit. By using magnetic particle chemiluminescent assay the sensitivity of determination of milk furaltadone metabolites is 0.01 ng/mL, and precision is less than 10%, and the cross-reactivity with metabolites furaltadone analogs is less than 0.5%, and the recovery reaches 95% to 103%. Chemiluminescence determination of magnetic particles in milk furaltadone metabolites is with high sensitivity, specificity and reaction speed, which has automated quant. monitoring on furaltadone metabolites, and also has broad application prospects.