Hengdian Group Hospital

The aim of this paper is to investigate significance of interferon releasing assays based on in vitro culture in auxiliary diagnosis of tuberculosis pleurisy.Venous blood was selected from 33 patients with tuberculous pleurisy and 36 patients with non-tuberculous pleurisy, we detected the interferon releasing level of haemocytes stimulated and cultured in vitro by the application of mycobacterium tuberculosis gamma interferon(TB-IGRA) releasing detection agentia in vitro, meanwhile, extracted pleural effusion for routine detection of adenosine deaminase(ADA), and compared it with the conventional method.The sensitivity, specificity and accuracy of detecting tuberculous pleurisy were resp. 93.9%, 88.9%, and 91.3% according to interferon release assay in vitro with whole blood cells as detection samples; through the comparison, interferon release test sensitivity is higher than that of the conventional ADA detection method(75.8%), and the difference was statistically significant(P<0.05).The TB-IGRA is a convenient and quick tool.It can improve the diagnostic efficiency of tuberculous pleurisy.

Objective: To study the protective effects of leonurine against white matter lesions in the brain of rats after chronic cerebral hypoperfusion and its mechanism.Methods: Adult male SD rats were randomly divided into sham group, chronic ischemia group and three leonurine groups with different dosages.The chronic cerebral ischemia model was established by ligating bilateral common catotid arteries.The rats were treated by i.p. injection with leonurine for 30 days, once a day.Luxol fast blue (LFB) staining, immunohistochem. staining and Western Blot anal. were used to observe the effects of leonurine on myelin sheath, expressions of APC and casepase-3 in corpus callosum.TUNEL staining was used to measure apoptosis.Results: In the model group, the LFB staining was weaker and the number of TUNEL-pos. neurons was increased.The results of immunohistochemistiy and Western Blot anal. showed the number of APC pos. OLGs was decreased and the protein expression level of caspase-3 was increased.Leonurine treatment may improve these changes.Conclusion: Leonurine could attenuate white matter injury due to chronical cerebral ischemia and its mechanism is associated with anti-apoptotic effect.

Objective: To observe and analyze the effect of combining traditional Chinese and western medicine nursing intervention on stability of chronic obstructive pulmonary disease patients.Methods: According to the random number table method, 106 cases of stabilization of chronic obstructive pulmonary disease patients in our hospital were grouped into control group and intervention group.Control group received oxygen inhalation, resolving phlegm and other conventional treatments and nursing.Intervention group was given combined Chinese and western medicine nursing intervention on the basis of routine therapy.We observed two groups' curative effect, FEV₁ and FEV₁FVC before and after nursing.Results: Before nursing, two groups' FEV₁, FEV₁/FVC, HDAC activity in sputum, TNF-α and IL-8 were not significant (P > 0.05).After nursing, the control group's FEV₁, FEV₁/FVC, HDAC activity in sputum, TNF-α and IL-8 were (1.76 ± 0.089) L, (71.21 ± 5.98)%, (1.86 ± 0.09) m/g and (127.82 ± 26.12) ng/L, (221.71 ± 27.86) ng/L, and those of the intervention group were (1.85 ± 0.091) L, (74.21 ± 6.39)%, (2.09 ± 0.15) m/g, (121.73 ± 18.72) ng/L and (209.91.71 ± 22.79) ng/L (P < 0.05 ).The effect comparison were significant between treatment group and control group (P < 0.05).Conclusion: Combined traditional Chinese and western medicine nursing can improve patients' clin. symptoms, promote patients recovery and improve the effect of clin. treatment.