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The retention behavior of a homologous series of polyaromatic hydrocarbons was evaluated on two phenyl-type stationary phases in reversed phase supercritical fluid chromatog. (SFC).These phases were the Synergi polar-RP phase and the Cosmosil 5PBB phase, both of which are polar end-capped and incorporate an ether in a Pr chain that tethers the Ph ring to the silica surface.The Cosmosil 5PBB phase also has five bromine atoms on the Ph ring.The retention capacity of the Cosmosil column was substantially greater than the Synergi column.However, selectivity on the Cosmosil column was effectively independent of the acetonitrile modifier composition in the CO₂ mobile phase, whereas, selectivity on the Synergi column was greatly affected by the acetonitrile modifier in the CO₂ mobile phase.The results from this study showed that selectivity and retention studies in HPLC cannot be used to predict selectivity and retention behavior in SFC.

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