To figure out current status of PCV3 prevalence, a national epidemiol. survey on PCV3 was conducted in this study.A total of 730 clin. tissue samples were collected from 60 pig farms in 17 provinces and 3 autonomous municipalities with different geog. distributions in China.The collected samples included lung, lymph node, tonsil, kidney, brain, spleen, heart, liver, and stillbirth.PCV3 DNA was detected using conventional PCR.One hundred and four out of 730 clin. samples were tested to be PCV3 pos. using PCR and the pos. rate was 14.25%.Since the previously published literatures about the PCV3 epidemiol. survey were mainly focused on provinces in South China, the results in this study for the first time indicated that PCV3 has widely spread to central and north parts of China and has nationwide distribution.

01 Apr 2018·Virologica SinicaQ2 · MEDICINE

A Duplex Real-Time PCR Assay for the Simultaneous Detection of Porcine Circovirus 2 and Circovirus 3

Q2 · MEDICINE

Article

Author: Li, Xiangdong ; Sun, Ming ; Qiao, Mingming ; Tian, Kegong

Porcine circoviruses (PCV) include PCV1, PCV2, and the new-emerging PCV3. PCV2 is pathogenic to pigs, but the pathogenicity of PCV3 in pigs is debatable. Recently, there have been frequent reports of PCV2 and PCV3 co-infections in clinical samples. Thus, it would be practical to develop a duplex PCR method to detect PCV2 and PCV3 simultaneously. In this study, specific primers and probes were designed to target PCV2 cap and PCV3 rep genes. A duplex real-time PCR method was then developed to detect the two viruses. The assay was found to be highly specific, sensitive, and reproducible for PCV2/3 without cross-reactions with other swine pathogens. The sensitivity of this assay was 2.9 copies for the PCV2 plasmid and 22.5 copies for the PCV3 plasmid. The established assay was then used to detect PCV2/3 infection in 340 clinical samples collected in the first half of 2017. The results showed that the co-infection rate of PCV2/3 in the samples was 27.6%. Our study provides an important tool that can be used to perform urgently needed surveys for the two porcine circoviruses to evaluate their impact on the swine industry.

01 Oct 2017·Infection, Genetics and Evolution

Whole genome characterization of a novel porcine reproductive and respiratory syndrome virus 1 isolate: Genetic evidence for recombination between Amervac vaccine and circulating strains in mainland China

Article

Author: Deng, Xiaoyu ; Liu, Qiaorong ; Sun, Ming ; Qiao, Mingming ; Chen, Nanhua ; Chen, Xizhao

Genotype 1 porcine reproductive and respiratory syndrome virus (PRRSV 1) have been continuously isolated in China in recent years. Complete genome sequences of these isolates are important to investigate the prevalence and evolution of Chinese PRRSV 1. Herein, we describe the isolation of a novel PRRSV 1 isolate, denominated HLJB1, in the Heilongjiang province of China. Complete genome sequencing of HLJB1 showed that it shares 90.66% and 58.21% nucleotide identities with PRRSV 1 and 2 prototypic strains Lelystad virus and ATCC VR-2332, respectively. HLJB1 has a unique 5-amino-acid insertion in nsp2, which has never been described in other PRRSV 1 isolates. Whole genome-based phylogenetic analysis revealed that all Chinese PRRSV 1 isolates are clustered in pan-European subtype 1 and can be divided into four subgroups. HLJB1 resides in the subgroup of BJEU06-1-like isolates but is also closely related to the Amervac-like isolates. Additionally, recombination analyses suggested that HLJB1 is a recombinant from the Amervac vaccine and the BJEU06-1 isolate. To our best knowledge, our results provide the first genetic evidence for recombination between Amervac vaccine and circulating strains. These findings are also beneficial for studying the origin and evolution of PRRSV 1 in China.

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