PIRT

Last update 08 May 2025

Basic Info

Synonyms

phosphoinositide interacting regulator of transient receptor potential channels, Phosphoinositide-interacting protein, PIRT

Introduction

Regulatory subunit of TRPV1, a molecular sensor of noxious heat and capsaicin. Positively regulates TRPV1 channel activity via phosphatidylinositol 4,5-bisphosphate (PIP2). Binds various phosphoinositide, including phosphatidylinositol 4,5-bisphosphate (PIP2), but not phosphatidylinositol (PI) (By similarity).

100 Clinical Results associated with PIRT

100 Translational Medicine associated with PIRT

0 Patents (Medical) associated with PIRT

Literatures (Medical) associated with PIRT

01 May 2024·Environmental Toxicology

An analysis of the prognostic role of reactive oxygen species‐associated genes in breast cancer

Article

Author: Deng, Junjie ; Cao, Hong ; Zhong, Yangyan ; Li, Wei ; Deng, Jian ; Li, Dan

AbstractBackgroundThis study aimed to type breast cancer in relation to reactive oxygen species (ROS), clinical indicators, single nucleotide variant (SNV) mutations, functional differences, immune infiltration, and predictive responses to immunotherapy or chemotherapy, and constructing a prognostic model.MethodsWe used uniCox analysis, ConsensusClusterPlus, and the proportion of ambiguous clustering (PAC) to analyze The Cancer Genome Atlas (TCGA) data to determine optimal groupings and obtain differentially expressed ROS‐related genes. Clinical indicators were then combined with the classification results and the Chi‐square test was used to assess differences. We further examined SNV mutations, and functional differences using gene set enrichment analysis (GSEA) analysis, the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, immune cell infiltration, and response to immunotherapy and chemotherapy. A prognostic model for breast cancer was constructed using these differentially expressed genes, immunotherapy or chemotherapy responses, and survival curves. RT‐qPCR was used to detect the differences in the expression of LCE3D, CA1, PIRT and SMR3A in breast cancer cell lines and normal breast epithelial cell line.ResultsWe identified two distinct tumor types with significant differences in ROS‐related gene expression, clinical indicators, SNV mutations, functional pathways, and immune infiltration. The response to specific chemotherapy drugs and immunotherapy treatments also documented significant differences. The prognostic model constructed with 16 genes linked to survival could efficiently divide patients into high‐ and low‐risk groups. The high‐risk group showed a poorer prognosis, higher tumor purity, distinct immune microenvironment, and lower immunotherapy response. RT‐qPCR results showed that LCE3D, CA1, PIRT and SMR3A are highly expressed in breast cancer.ConclusionOur methodical examination presented an enhanced insight into the molecular and immunological heterogeneity of breast cancer. It can contribute to the understanding of prognosis and offer valuable insights for personalized treatment strategies. Further, the prognostic model can potentially serve as a powerful tool for risk stratification and therapeutic decision‐making in clinical settings.

01 May 2022·The FASEB Journal

SPARC: Visualization of genetically‐labeled vagal and spinal afferent subsets innervating the mouse lung

Article

Author: Bahia, Parmvir ; Hadley, Stephen ; Taylor‐Clark, Thomas ; Patil, Mayur ; Kim, Seol‐Hee

Pulmonary functions are controlled by afferent nerves which convey peripheral information to the central nervous system. Cell bodies of these afferent nerves are found predominantly in the vagal ganglia (VG) with some in the dorsal root ganglia (DRG). These neurons are highly heterogeneous based on their developmental origins, anatomical sources, and physiochemical properties. Vagal ganglia are composed of nodose (placode origin), and jugular ganglia (neural crest origin). Most vagal afferent nerves innervating the lung are unmyelinated C‐fibers which are activated by capsaicin, the selective agonist of transient receptor potential vanilloid 1 (TRPV1). TRPV1 detects noxious stimuli and its activation results in defensive reflexes. Both nodose and jugular ganglia have TRPV1+ nociceptive C‐fiber but it is not currently known where they terminate within the lung. In addition, there is lack of information regarding pulmonary afferent nerves projecting from the DRG, which also are derived from the neural crest. Here, we used cell‐type specific Cre knock‐in strains in combination with injections with adeno‐associated viral vectors (AAV) carrying a Cre‐sensitive reporter allele to label specific subsets of vagal and DRG afferent nerves in lung. Pirt‐cre (marker for all sensory neurons), TRPV1‐cre (nociceptors) and Tac1‐cre (jugular, DRG) strains received unilateral injections of AAV9‐flex‐EGFP into nodose ganglia, and/or AAV9‐flex‐tdTomato into thoracic DRG (between T1‐T3). VG, DRG and lung were collected 4 weeks post‐injection and cryosectioned. Native fluorescent signals were amplified using anti‐DsRed and anti‐GFP immunoreactivity, and images were taken using an Andor Dragonfly spinning disk confocal microscope. Viral transfection was confirmed by expression of GFP in VG or tdTomato in DRG. Lung images were analyzed for VG/DRG nerve innervations based on diameters of airways (small: ≤175 µm, medium: 175‐376 µm, large: ≥376 µm). >95% of the conducting airways was innervated by vagal Pirt+ afferent nerves. About half of the conducting airways also had vagal Pirt+ fibers which projected out into the alveolar regions (mean distance 200±50 µm). Only 20% of the airways were innervated by Pirt+ DRG fibers, none of which projected into the alveolar regions. Vagal‐Pirt(+) nerves innervate most of the airways regardless of size, but DRG‐Pirt(+) nerves innervate mostly large diameter airways. ~75% of airways are innervated by vagal TRPV1+ afferents, some of which project into the alveolar regions. There is virtually no TRPV1+ innervation of the lung projected from the DRG. ~75% of large/medium sized airways were innervated by vagal originating nerves in Tac1‐cre, but none of these fibers projected into the alveolar spaces. Some large diameter airways were also innervated by Tac1+ fibers from DRG neurons. Together, our approach with the unilateral intraganglionic injections of AAV, into the VG and DRG, carrying a cre‐dependent reporter allele allows identification of specific subsets of afferent nerves in the VG and DRG innervating the lung.

01 Mar 2022·eneuroQ3 · MEDICINE

Mapping of the Sensory Innervation of the Mouse Lung by Specific Vagal and Dorsal Root Ganglion Neuronal Subsets

Q3 · MEDICINE

ArticleOA

Author: Butler, Shane G ; Hadley, Stephen H ; Taylor-Clark, Thomas E ; Patil, Mayur J ; Madaram, Meghana ; Kim, Seol-Hee ; Bahia, Parmvir K

The airways are densely innervated by sensory afferent nerves, whose activation regulates respiration and triggers defensive reflexes (e.g., cough, bronchospasm). Airway innervation is heterogeneous, and distinct afferent subsets have distinct functional responses. However, little is known of the innervation patterns of subsets within the lung. A neuroanatomical map is critical for understanding afferent activation under physiological and pathophysiological conditions. Here, we quantified the innervation of the mouse lung by vagal and dorsal root ganglion (DRG) sensory subsets defined by the expression of Pirt (all afferents), 5HT₃ (vagal nodose afferents), Tac1 (tachykinergic afferents), and transient receptor potential vanilloid 1 channel (TRPV1; defensive/nociceptive afferents) using Cre-mediated reporter expression. We found that vagal afferents innervate almost all conducting airways and project into the alveolar region, whereas DRG afferents only innervate large airways. Of the two vagal ganglia, only nodose afferents project into the alveolar region, but both nodose and jugular afferents innervate conducting airways throughout the lung. Many afferents that project into the alveolar region express TRPV1. Few DRG afferents expressed TRPV1. Approximately 25% of blood vessels were innervated by vagal afferents (many were Tac1+). Approximately 10% of blood vessels had DRG afferents (some were Tac1+), but this was restricted to large vessels. Lastly, innervation of neuroepithelial bodies (NEBs) correlated with the cell number within the bodies. In conclusion, functionally distinct sensory subsets have distinct innervation patterns within the conducting airways, alveoli and blood vessels. Physiologic (e.g., stretch) and pathophysiological (e.g., inflammation, edema) stimuli likely vary throughout these regions. Our data provide a neuroanatomical basis for understanding afferent responses in vivo.

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