KATNA1

Last update 08 May 2025

Basic Info

Synonyms

katanin catalytic subunit A1, katanin p60 (ATPase containing) subunit A 1, katanin p60 (ATPase-containing) subunit A 1

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Introduction

Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.

100 Clinical Results associated with KATNA1

100 Translational Medicine associated with KATNA1

0 Patents (Medical) associated with KATNA1

Literatures (Medical) associated with KATNA1

01 Mar 2025·Biochemical and Biophysical Research Communications

The interaction between KATNA1 and CRMP3 modulates microtubule dynamics and neurite outgrowth

Article

Author: Lin, Hongsheng ; Xie, Zhiyao ; Tan, Minghui ; Song, Wei ; Meng, Zhichao ; Liang, Yaozhong ; Ma, Ao ; Lei, Changbin ; Zhu, Jiehao

The polymerization and severing of microtubules are fundamental to the growth and branching of neurites in hippocampal neurons. The catalytic ATPase-containing A-subunit of katanin p60 (p60, KATNA1) promotes growth and development of hippocampal neurites by severing microtubules, while collapsing response mediator protein 3 (CRMP3) assembles microtubules to regulate neurite outgrowth. However, whether microtubule severing and assembling proteins would work together to regulate neurite outgrowth, especially for KATNA1 and CRMP3 remains to be elucidated. In this study, we revealed the interaction between KATNA1 and CRMP3 through GST-pulldown and co-immunoprecipitation assays and identified the binding domains between KATNA1 and CRMP3 as the MIT of KATNA1 (residues 1-77) and the D region of CRMP3 (residues 64-413). Furthermore, we demonstrated that CRMP3 enhances the microtubule-severing efficiency of KATNA1. In cultured hippocampal neurons, overexpression of KATNA1 and CRMP3 increased neurite length and branch number, and co-expression of both proteins further enhanced the promoting effect. Moreover, genetic knockout of KATNA1 or/and CRMP3 significantly inhibited neurite outgrowth. Overall, our data suggest that the CRMP3 interaction enhances the severing activity of KATNA1, thereby promoting hippocampal neurite outgrowth.

15 Nov 2023·Development

The katanin A-subunits KATNA1 and KATNAL1 act co-operatively in mammalian meiosis and spermiogenesis to achieve male fertility

Article

Author: Wozniak, Kathryn ; O'Connor, Anne E. ; O'Bryan, Moira K. ; Dunleavy, Jessica E. M. ; Merriner, D. Jo ; Houston, Brendan J. ; Nguyen, Joseph ; Schittenhelm, Ralf B. ; Graffeo, Maddison

ABSTRACTKatanins, a class of microtubule-severing enzymes, are potent M-phase regulators in oocytes and somatic cells. How the complex and evolutionarily crucial, male mammalian meiotic spindle is sculpted remains unknown. Here, using multiple single and double gene knockout mice, we reveal that the canonical katanin A-subunit KATNA1 and its close paralogue KATNAL1 together execute multiple aspects of meiosis. We show KATNA1 and KATNAL1 collectively regulate the male meiotic spindle, cytokinesis and midbody abscission, in addition to diverse spermatid remodelling events, including Golgi organisation, and acrosome and manchette formation. We also define KATNAL1-specific roles in sperm flagellum development, manchette regulation and sperm-epithelial disengagement. Finally, using proteomic approaches, we define the KATNA1, KATNAL1 and KATNB1 mammalian testis interactome, which includes a network of cytoskeletal and vesicle trafficking proteins. Collectively, we reveal that the presence of multiple katanin A-subunit paralogs in mammalian spermatogenesis allows for ‘customised cutting’ via neofunctionalisation and protective buffering via gene redundancy.

01 Nov 2023·Cytoskeleton

A potential posttranscriptional regulator for p60‐katanin: miR‐124‐3p

Article

Author: Yildiz, Aysegul ; Korulu, Sirin ; Kaya, Yesim ; Tunoglu, Ezgi Nurdan Yenilmez

AbstractKatanin is a microtubule severing protein belonging to the ATPase family and consists of two subunits; p60‐katanin synthesized by the KATNA1 gene and p80‐katanin synthesized by the KATNB1 gene. Microtubule severing is one of the mechanisms that allow the reorganization of microtubules depending on cellular needs. While this reorganization of microtubules is associated with mitosis in dividing cells, it primarily takes part in the formation of structures such as axons and dendrites in nondividing mature neurons. Therefore, it is extremely important in neuronal branching. p60 and p80 katanin subunits coexist in the cell. While p60‐katanin is responsible for cutting microtubules with its ATPase function, p80‐katanin is responsible for the regulation of p60‐katanin and its localization in the centrosome. Although katanin has vital functions in the cell, there are no known posttranscriptional regulators of it. MicroRNAs (miRNAs) are a group of small noncoding ribonucleotides that have been found to have important roles in regulating gene expression posttranscriptionally. Despite being important in gene regulation, so far no microRNA has been experimentally associated with katanin regulation. In this study, the effects of miR‐124‐3p, which we detected as a result of bioinformatics analysis to have the potential to bind to the p60 katanin mRNA, were investigated. For this aim, in this study, SH‐SY5Y neuroblastoma cells were transfected with pre‐miR‐124‐3p mimics and pre‐mir miRNA precursor as a negative control, and the effect of this transfection on p60‐katanin expression was measured at both RNA and protein levels by quantitative real‐time PCR (qRT‐PCR) and western blotting, respectively. The results of this study showed for the first time that miR‐124‐3p, which was predicted to bind p60‐katanin mRNA by bioinformatic analysis, may regulate the expression of the KATNA1 gene. The data obtained within the scope of this study will make important contributions in order to better understand the regulation of the expression of p60‐katanin which as well will have an incontrovertible impact on the understanding of the importance of cytoskeletal reorganization in both mitotic and postmitotic cells.

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KATNA1

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