Aims:Sevoflurane and propofol are the most commonly used anesthetics in surgery.
In this study, we aim to explore and clarify the function of sevoflurane and propofol in colorectal
cancer.Methods:Cell counting kit-8, colony formation, western blot, and transwell assays were performed
to determine cell proliferation, apoptosis, ferroptosis, invasion, and migration. We performed
overexpression experiments to detect the underlying molecular mechanism of sevoflurane
and propofol. The genes related to epithelial-mesenchymal transition were measured by
western blot.Results:We discovered that sevoflurane and propofol co-treatment exerted more anti-tumor activities
than just sevoflurane or propofol treatment in colorectal cancer cells in vitro. Mechanistically,
our data showed that sevoflurane and propofol-induced apoptosis and ferroptosis and inhibited
cell proliferation, invasion, and migration. Additionally, TM2D1 was considered a target
of sevoflurane and propofol, and TM2D1 overexpression reversed the effect of sevoflurane and
propofol on colorectal cancer cell biology behaviors.Conclusion:Our results showed a novel anti-tumor mechanism of sevoflurane and propofol in
colorectal cancer cells, and TM2D1 might be an underlying therapeutic target for treating colorectal
cancer patients.other:Our results showed novel anti-tumor mechanism of sevoflurane and propofol in colorectal cancer cells, and TM2D1 might be an underlying therapeutic target for treatment of colorectal cancer patients.