GNB5

Last update 08 May 2025

Basic Info

Synonyms

G protein subunit beta 5, GB5, Gbeta5

+ [3]

Introduction

Enhances GTPase-activating protein (GAP) activity of regulator of G protein signaling (RGS) proteins, such as RGS7 and RGS9, hence involved in the termination of the signaling initiated by the G protein coupled receptors (GPCRs) by accelerating the GTP hydrolysis on the G-alpha subunits, thereby promoting their inactivation (PubMed:27677260). Increases RGS7 GTPase-activating protein (GAP) activity, thereby regulating mood and cognition (By similarity). Increases RGS9 GTPase-activating protein (GAP) activity, hence contributes to the deactivation of G protein signaling initiated by D(2) dopamine receptors (PubMed:27677260). May play an important role in neuronal signaling, including in the parasympathetic, but not sympathetic, control of heart rate (By similarity).

100 Clinical Results associated with GNB5

100 Translational Medicine associated with GNB5

0 Patents (Medical) associated with GNB5

186

Literatures (Medical) associated with GNB5

31 Dec 2025·Renal Failure

Key RNA-binding proteins in renal fibrosis: a comprehensive bioinformatics and machine learning framework for diagnostic and therapeutic insights

Article

Author: Zhang, Binghan ; Chen, Jie ; Huang, Qixuan ; Ren, Ziyu ; Liu, Dongfang ; Fang, Ronghua

BACKGROUNDRenal fibrosis is a critical factor in chronic kidney disease progression, with limited diagnostic and therapeutic options. Emerging evidence suggests RNA-binding proteins (RBPs) are pivotal in regulating cellular mechanisms underlying fibrosis.METHODSUtilizing an extensive GEO dataset (175 renal fibrosis and 99 normal kidney samples), we identified and validated key RBPs through integrated bioinformatics and machine learning approaches, including lasso and logistic regression models. Differentially expressed genes were analyzed for pathway enrichment using Gene Ontology and KEGG. Single-cell RNA sequencing delineated cell-specific RBP expression, and a murine unilateral ureteral obstruction (UUO) model provided experimental validation.RESULTSA diagnostic model incorporating five RBPs (FKBP11, DCDC2, COL6A3, PLCB4, and GNB5) achieved high accuracy (AUC = 0.899) and robust external validation. These RBPs are implicated in immune-mediated pathways such as cytokine signaling and inflammatory responses. Single-cell analysis highlighted their expression in specific renal cell populations, underscoring functional diversity. Immunofluorescence linked FKBP11 with macrophage infiltration, suggesting its potential as a therapeutic target.CONCLUSIONhis study identifies novel RBPs associated with renal fibrosis, advancing the understanding of its pathogenesis and offering actionable biomarkers and therapeutic targets. The integration of bioinformatics and machine learning emphasizes their translational potential in kidney care.

01 Apr 2025·Cytotherapy

Ganglioside expression delineates human mesenchymal stem/stromal cell populations derived from different tissue sources

Article

Author: Guérardel, Yann ; Coddeville, Bernadette ; Yamakawa, Nao ; Groux-Degroote, Sophie ; Sackstein, Robert ; Martin, Kyle

Prior studies have indicated that human embryonic stem cells can be distinguished from those of other mammals based on variable expression of a class of membrane glycolipids known as glycosphingolipids (GSLs), raising the question as to whether GSL display could be utilized to phenotypically define subsets of human adult stem cell populations. Adult stem cells known as "mesenchymal stem/stromal cells" (MSCs) have shown immense promise in therapeutic applications for a variety of clinical indications. Most commonly, these cells are harnessed and then culture-expanded from bone-marrow (BM-MSCs) or from adipose tissue (A-MSCs) sources. Though operational differences exist between human BM-MSCs and A-MSCs, no surface markers have been characterized to date that distinguish these as distinct subsets of culture-expanded human adult stem cells. Accordingly, we isolated GSLs from primary cultures of marrow- and adipose-derived human MSCs and an unbiased screen was performed by mass spectrometry (via matrix-assisted laser desorption/ionization (MALDI)-quadrupole ion trap (QIT)-time-of-flight (TOF), hence, via "MALDI-QIT-TOF") to analyze all component glycans. Flow cytometry was then undertaken to assess the relative levels of expression of MS-defined glycan determinants, followed by RT-qPCR to measure transcripts of genes encoding key enzymes involved in glycolipid biosynthesis. Notably, our data indicate that neither BM- nor A-MSCs display any significant level of either lacto-series or neolacto-series GSLs, but distinct differences exist in GSL species among A-MSCs and BM-MSCs: while both cell types express GSLs of the ganglio- and the globo-series, the ganglio-series GSLs GD3 and GD2 and the globo-series GSL SSEA-4 (also known as sialylGb5) are dominantly expressed only among human BM-MSCs. These structural features are shaped by divergent patterns of glycosyltransferase gene expression, with striking differences between BM- and A-MSCs in the expression of transcripts encoding GD3 synthase, GM2/GD2 synthase, and Gb5 synthase. Importantly, expression of GD3, GD2, and SSEA-4 is markedly diminished on differentiation of BM-MSCs, and co-cultures of A-MSCs and BM-MSCs show that the expression of GD3, GD2, and SSEA-4 is a cell-intrinsic feature of BM-MSCs. These data stratify the glycosignature(s) of human MSCs derived from different tissue sources, provide direct evidence that expression of these structures is cell stage-/lineage-specific, unveil the mechanistic basis of the differential expression of these glycan determinants, and draw attention to how knowledge of the MSC glycosignature can impact cytotherapeutic strategies.

01 Mar 2025·Journal of Pharmacological and Toxicological Methods

Identification of gene expression change associated with isoflurane anesthesia and neurocognitive disorders using bioinformatics methods

Article

Author: Fu, Hua ; Zhang, Ying ; Yu, Jialu ; Liu, Yang

BACKGROUNDIsoflurane, a commonly used anesthetic, has been linked to neurocognitive dysfunction (NCD). However, the precise relationship between isoflurane anesthesia and NCD remains unclear.METHODSDatasets related to isoflurane anesthesia were obtained from a public database. The hub genes of isoflurane anesthesia were selected using logistic regression with the least absolute shrinkage and selection operator (LASSO). Human neuroblastoma cell line SH-SY5Y was induced to differentiate into terminal neuron-like cells.RESULTSThe signaling pathways involved in isoflurane anesthesia and NCD were subjected to analysis, resulting in the identification of 35 signaling pathways, including the PI3K/AKT pathway, the AGE-RAGE signaling pathway and the apelin signaling pathway, which were found to be associated with both NCD and isoflurane anesthesia. A total of nine cross-expressed genes (Nfe2l2, Fgf2, Edn1, Spp1, Hmox1, Picalm, Gnb5, Eif2s1 and Gls) were identified between isoflurane anesthesia and NCD. The LASSO logistic regression model was employed to identify three hub genes (Fgf2, Gnb5, Spp1). The expression of these three hub genes was validated using other expression datasets. In human neuron-like cells, isoflurane also significantly affected the expression of three corresponding human homologous genes. The expression trends of these three genes in human cells were consistent with the expression in rat brains after isoflurane treatment, providing further evidence for the rationality of the three hub genes.CONCLUSIONSThe present study revealed key candidate genes and related functional signaling pathways through bioinformatics analysis and cell experiments, which may serve as the basis for isoflurane-related NCD.

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