NKAP

Last update 08 May 2025

Basic Info

Synonyms

FLJ22626, NF-kappa-B-activating protein, NFKB activating protein

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Introduction

Acts as a transcriptional repressor (PubMed:14550261, PubMed:19409814, PubMed:31587868). Plays a role as a transcriptional corepressor of the Notch-mediated signaling required for T-cell development (PubMed:19409814). Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter.

100 Clinical Results associated with NKAP

100 Translational Medicine associated with NKAP

0 Patents (Medical) associated with NKAP

Literatures (Medical) associated with NKAP

01 Jan 2025·Journal of Stomatology, Oral and Maxillofacial Surgery

NKAP: A new m6A RNA binding protein predicts prognosis and immunotherapy response in head and neck squamous cell carcinoma

Article

Author: Manicka Vasagam, Jeevitha ; Arumugam, Paramasivam ; Jayaseelan, Vijayashree Priyadharsini

OBJECTIVEThis study aimed to investigate whether NKAP (nuclear factor κB activating protein) serves as a prognostic marker and predictive biomarker for immunotherapy response in head and neck squamous cell carcinoma (HNSCC).METHODSA retrospective cohort study combined with in vitro analyses was conducted. NKAP mRNA expression levels were assessed in 520 HNSCC tumor tissues and 44 normal tissues from the TCGA dataset and validated in a clinical cohort (n = 32). Clinical correlations with overall survival and immunotherapy outcomes were analyzed. The key pathological variables included tumor stage, grade, HPV status, and TP53 mutation. Appropriate statistics were calculated at a significant level (P<0.05).RESULTSIn this study, we have collected tissue samples from HNSCC patients (mean age: 52.65 ± 7.76; males 66%, females 34%). NKAP was upregulated in HNSCC tissues compared to adjacent normal tissues (P < 0.001). The high expression of NKAP correlated with advanced tumor stage, grade, and reduced survival (P < 0.05). High expression levels of NKAP were also associated with anti-PD-L1 therapy response (P < 0.05). Functional enrichment analysis revealed NKAP involvement in cell cycle regulation, mRNA processing, and chromatin remodeling pathways critical for cancer progression.CONCLUSIONNKAP represents a promising prognostic marker and therapeutic target for immunotherapy in HNSCC. Prospective studies are warranted to confirm these findings.

01 Dec 2024·Fish & Shellfish Immunology

Hepcidin defense patterns in intestine of mandarin fish (Siniperca chuatsi) against Aeromonas hydrophila infection

Article

Author: Guo, Ning-xin ; Zhao, Jin-Liang ; Yao, Xiao-li ; Hu, Miao ; Cai, Kang-Ning ; Tola, Temesgen ; Gao, Jin-hua ; Gao, Jin-Hua ; Zhao, Jin-liang ; Yao, Xiao-Li ; Xue, Wen-Bo ; Guo, Ning-Xin ; Xue, Wen-bo ; Cai, Kang-ning

Bacterial septicemia caused by Aeromonas hydrophila is one of the common bacterial diseases in aquaculture. Antimicrobial peptides, including hepcidin, are key components of the innate immune system in fish, playing a role in defense against pathogens. This study investigated the defense patterns of hepcidin in mandarin fish (Siniperca chuatsi) following A. hydrophila infection using gene expression analysis and in vitro antibacterial assays. We measured changes in the expression of iron metabolism-related genes (hepcidin, fpn, ftn, tf, tfr1) and immune-related genes (il-1β, il-6, il-8, il-10, tnf-α, socs3, nkap, tlr1, tlr2) in the intestine post-infection. MBC experiment demonstrated that the hepcidin synthetic peptide has an inhibitory effect on the growth of V. parahaemolyticus (32 μg/ml), A. hydrophilus (64 μg/ml), and F. columnaris (128 μg/ml), but not E. tarda (>256 μg/ml). After A. hydrophilus challenge, fpn, tf and tfr1 with peak expression at 24 hpi (2.75), 12 hpi (4.43) and 6 hpi (7.41), respectively. Hepcidin and ftn expression was highest at 48 hpi (115.01) and 72 hpi (4.16). The Fe²⁺ content peaked at 6 hpi (2.64 μmol/l) and reached its lowest at 12 hpi (1.12 μmol/l) in the intestine. After pathogen challenge, il-1β, il-8, socs3, tlr2, and hepcidin showed trends of increased and then decreased, with peak expression at 72 hpi (5.13, 37.05, 3.08) and 48 hpi (5.35, 115.01), respectively. These findings suggested that hepcidin plays a key role in the defense against A. hydrophila: initially restricting bacterial growth through iron metabolism (0-48 hpi), and later modulating immune responses via the TNF (by inducing il-1β and socs3) and Toll-like receptor pathways (by inducing il-8 and tlr2) (48-96 hpi). This study provides novel insights into the immune function of hepcidin in fish and its potential application in managing bacterial infections in aquaculture.

01 Dec 2024·Journal of Genetics and Genomics

Capn3b-deficient zebrafish model reveals a key role of autoimmune response in LGMDR1

Article

Author: Huang, Delai ; Fang, Weihuan ; Gao, Ce ; Shan, Ying ; Shi, Hui ; Peng, Jinrong ; Zhao, Shuyi ; Chen, Yayue ; Chen, Jun ; Xie, Aixuan

Mutations in calcium-dependent papain-like protease CALPAIN3 (CAPN3) cause Limb-Girdle Muscular Dystrophy Recessive Type 1 (LGMDR1), the most common limb-girdle muscular dystrophy in humans. In addition to progressive muscle weakness, persistent inflammatory infiltration is also a feature of LGMDR1. Despite the underlying mechanism remaining poorly understood, we consider that it may relate to the newly defined role of CAPN3/Capn3b in the nucleolus. Here, we report that the loss of function of zebrafish capn3b, the counterpart of human CAPN3, induces an autoimmune response akin to that in LGMDR1 patients. capn3b mutant larvae are more susceptible to Listeria monocytogenes injection, characterized by recruiting more macrophages. Under germ-free conditions, transcriptome analysis of the capn3b mutant muscle reveals a significant upregulation of the chemokine-production-related genes. Coincidently, more neutrophils are recruited to the injury site imposed by either muscle stabbing or tail fin amputation. Nucleolar proteomic analysis and enzymatic assays reveal NKAP, an activating factor of the NF-κB pathway, to be a target of CAPN3. We conclude that the accumulation of Nkap and other factors in the capn3b mutant may be involved in the over-activation of innate immunity. Our studies indicate that the zebrafish capn3b mutant is a powerful model for studying the immunity-related progression of human LGMDR1.

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