MAN2C1

Last update 08 May 2025

Basic Info

Synonyms

Alpha mannosidase 6A8B, Alpha-D-mannoside mannohydrolase, Alpha-mannosidase 2C1

+ [4]

Introduction

Cleaves alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues on cytoplasmic free oligosaccharides generated by N-glycoprotein degradation pathways.

100 Clinical Results associated with MAN2C1

100 Translational Medicine associated with MAN2C1

0 Patents (Medical) associated with MAN2C1

177

Literatures (Medical) associated with MAN2C1

24 Apr 2025·The Journal of Physical Chemistry B

Effect of Mutations on Smlt1473 Binding to Various Substrates Using Molecular Dynamics Simulations

Article

Author: Klauda, Jeffery B. ; Mondal, Kinjal ; Felton, Samantha ; Berger, Bryan W.

Smlt1473 is a polysaccharide lyase from Stenotrophomonas maltophilia whose crystal structure was solved recently by using X-ray crystallography. There was an effort to study the effect of mutations on the activity of Smlt1473 binding to various substrates like hyaluronic acid (HA), mannuronic acid (ManA), and alginate. In this study, we use molecular docking and molecular dynamics simulations to investigate the effect of binding of various substrates (HA and ManA) to Smlt1473 and two of its mutants H221F and R312L. We further studied the stability in the binding of Smlt1473 to its various substrates as well as the role of fluctuations. Machine learning-based clustering algorithms were used to group the entire simulation trajectory into various stable states. The molecular interactions of Smlt1473 with the substrates were calculated, and the importance of specific residues was tested with observed activity assays due to residue mutations. Overall, we find that R218 plays an important role in substrate binding and thus impacting the activity due to the H221F mutant and R/L312 itself plays an important role in the R312 mutation. In addition, we have also found three more residues─K56, R107, and R164─as important for substrate binding, which we further proceed to confirm using wet lab mutagenesis studies.

01 Apr 2025·iScience

Enriched pathways in gut microbiome predict response to immune checkpoint inhibitor treatment across demographic regions and various cancer types

Article

Author: Ge, Siyi ; Wang, Guoping ; Cai, Xunhui ; Salgado, Katia ; Yu, Hui ; Wu, Meng ; Cho, Jennifer Y ; Xia, Tian ; Futreal, P Andrew ; Cheng, Chao ; Hsiao, Ansel ; Zhang, Jianjun ; Chen, Yaobing ; Yang, Dehua ; Chen, Lijun ; Liu, Yufeng ; Gibbons, Don ; Shao, Jianbo ; Ji, Fenghu ; Sepesi, Boris

Understanding the effect of gut microbiota function on immune checkpoint inhibitor (ICI) responses is urgently needed. Here, we integrated 821 fecal metagenomes from 12 datasets to identify differentially abundant genes and construct random forest models to predict ICI response. Gene markers demonstrated excellent predictive performance, with an average area under the curve (AUC) of 0.810. Pathway analyses revealed that quorum sensing (QS), ABC transporters, flagellar assembly, and amino acid biosynthesis pathways were enriched between responders (R) and non-responders (NRs) across 12 datasets. Furthermore, luxS, manA, fliC, and trpB exhibited consistent changes between R and NR across 12 datasets. Follow-up microbiota transplant experiments showed that inter-species signaling by different QS autoinducer-2 (AI-2) molecules (synthesized by luxS) can act on overall community function to promote the colonization of Akkermansia muciniphila, which is associated with superior ICI responses. Together, our data highlight the role of gut microbiota function in modulating the microbiome and antitumor immunity.

01 Sep 2024·Plant Disease

Onion (Allium cepa) as a New Host for ‘Candidatus Arsenophonus phytopathogenicus’ in Germany

Article

Author: Therhaag, Eva ; Gross, Jürgen ; Schneider, Bernd ; Ulrich, Roswitha

Onion (Allium cepa L.) is the most produced vegetable after tomato worldwide and is grown on about 15,000 ha in Germany. In Lampertheim, Hesse in southwest Germany (49°40'02.3"N, 8°26'00.0"E) bulbs of the cultivar ‘Red Baron F1’ were harvested in September 2023 in an apparently healthy state. Four months later some of the onions showed rotting symptoms, which could not be assigned to a known storage disease. At first, the bulbs became glassy, later they showed soft rot. They originated from a field located in a growing region severely affected by “Syndrome Basses Richesses” (SBR). ‘Candidatus Arsenophonus phytopathogenicus’ as well as ‘Candidatus Phytoplasma solani’ are associated with this disease in sugar beet (Gatineau et al. 2002). Moreover, ‘Ca. A. phytopathogenicus’ was recently reported in association of bacterial wilt and yellowing in potato (Behrmann et al. 2023). Both phloem-restricted bacteria are vectored by the polyphagous planthopper Pentastiridius leporinus (Therhaag et al. 2024), which is highly abundant in this region. To examine, if the unknown symptoms in onion might be related to the presence of these pathogens, DNA of 69 bulbs showing a different degree of softening were analyzed. The samples were tested for the presence of ‘Ca. Phytoplasma solani’ in a TaqMan assay (Behrmann et al. 2022). All showed negative results. To demonstrate the presence of ‘Ca. A. phytopathogenicus’, universal and genus-specific primers for the amplification of 16S rDNA and a real-time qPCR assay amplifying an hsp20 fragment were employed (Christensen et al. 2004, Zübert and Kube 2021). Two bulbs of the five positive samples were in an apparently healthy state, the other three showed light to moderate softening symptoms. The 16S rDNA fragments from two samples were sequenced on both strands and aligned. Both fragments were homologous. One fragment of 1474 bp fragment showing 100% homology to the 16S rDNA from SBR (accession no. AY057392) was submitted to GenBank (accession no. PP400342). Other taxa of ‘Ca. Arsenophonus’ showed 16S rDNA homologies of less than 99.3 %. To corroborate the finding onion samples were subjected to PCR reactions employing genus-specific primers for the conserved tufB, secY and manA gene, which had been derived from multiple alignments of ‘Ca. A. spp’ sequence submissions (Sela et al. 1989, Lee et al. 2010). The tufB, secY and manA primers amplified fragments of about 980 bp, 640 bp and 930 bp, respectively, from all previously positive samples. Samples which had been tested negative for ‘Ca. P. phytopathogenicus’ remained negative. Fragments from two accessions were sequenced and the sequences from both isolates were 100 % identical. A BLAST search of the partial tufB gene (acc. no. PP950434) showed 98.57 % sequence identity to a yet unnamed Arsenophonus endosymbiont (acc. no. OZ026540) and 91.85 to 91.83 % to ‘Ca. A. nasoniae’ and ‘Ca. A. apicola’, respectively. A similar result was obtained for the partial secY sequence (acc. no. PP950433). The manA sequence (acc. no. PP942231) was identical to a partial sequence of ‘Ca. A. phytopathogenicus’ strain HN (acc. no. OK335757) and 97.42 % to ‘Ca. A. nasoniae and about 87 % to related Arsenophonus species. The finding of ‘Ca. A. phytopathogenicus’ in onion is novel and might indicate an expanding host range of vector and pathogen in the regional crop rotation. As a correlation between the pathogen and the soft rot symptom is unclear at present, further investigations are needed.

Analysis

Perform a panoramic analysis of this field.

view full example data

AI Agents Built for Biopharma Breakthroughs

Accelerate discovery. Empower decisions. Transform outcomes.

Get started for free today!

Accelerate Strategic R&D decision making with Synapse, PatSnap’s AI-powered Connected Innovation Intelligence Platform Built for Life Sciences Professionals.

Start your data trial now!

Synapse data is also accessible to external entities via APIs or data packages. Empower better decisions with the latest in pharmaceutical intelligence.

Bio

Bio Sequences Search & Analysis

Chemical

Chemical Structures Search & Analysis

MAN2C1

Basic Info

Related

Analysis