SPACA1

Last update 08 May 2025

Basic Info

Synonyms

SAMP32, SPACA1, Sperm acrosomal membrane-associated protein 32

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Introduction

Plays a role in acrosome formation and establishment of normal sperm morphology during spermatogenesis (PubMed:34172998). Important for male fertility (PubMed:11870081).

100 Clinical Results associated with SPACA1

100 Translational Medicine associated with SPACA1

0 Patents (Medical) associated with SPACA1

Literatures (Medical) associated with SPACA1

01 Mar 2025·Journal of Advanced Research

Spatiotemporal translation of sperm acrosome associated proteins during early capacitation modulates sperm fertilizing ability

Article

Author: Pang, Won-Ki ; Rahman, Md Saidur ; Park, Yoo-Jin ; Pang, Myung-Geol ; Ryu, Do-Yeal

INTRODUCTIONDespite the lack of essential cytoplasmic organelles in mature spermatozoa, which creates unfavorable conditions for transcription and translation, the presence of various mRNA and proteins during capacitation suggests potential for de novo protein synthesis.OBJECTIVESWe applied a metabolic labeling method using a fluorescent noncanonical amino acid tagging system (FUNCAT) and proximity ligation method (PLA) in normal and reduced fertility spermatozoa to detect different translation phenomena during sperm capacitation according to their fertility.METHODSWe explored different proteome changes in spermatozoa according to the time-sequential capacitation process (0, 20, 40, 60, and 120 min) between normal [average fertility rate (FR) = 77.44 % ± 1.51] and reduced fertility (average FR = 58.57 % ± 1.64) spermatozoa bull spermatozoa, as the representative male fertility models owing to their broad spectrum of fertility phenotypes. Moreover, the FUNCAT/PLA method was used to detect and visualize different translation phenomena during sperm capacitation according to fertility.RESULTSWe found that sperm-associated protein (SPACA) 1 and SPACA5 were newly synthesized in the head of normal-fertility spermatozoa, whereas a lack of newly synthesized proteins in the head and a relatively earlier loss of SPACA1 and SPACA5 were observed in the reduced-fertility spermatozoa. Moreover, the mitochondrial translation inhibitor, chloramphenicol, partially inhibited sperm translation and delayed translocation, suggesting that mitochondria participate in sperm translation.CONCLUSIONOur results unveil time-sequential microenvironmental changes in sperm proteomes during capacitation, which lead to the orchestra of proteins that complete fertilization. Fertile spermatozoa are selected through inter-competition during the journey of fertilization in the female reproductive tract. This study provides an overview of how translation dynamics acts on the sperm selection and influence the evolution of sperm fertility.

01 Feb 2025·Theriogenology

Sperm acrosome-associated 1 (SPACA1) mRNA and protein molecules deficiency indicate low fertility and semen quality of Bali bulls (Bos sondaicus)

Article

Author: Nugraha, Chairdin Dwi ; Iskandar, Hikmayani ; Pamungkas, Fitra Aji ; Purwantara, Bambang ; Sonjaya, Herry ; Hasbi, Hasbi ; Said, Syahruddin ; Suyadi, Suyadi ; Agil, Muhammad ; Memili, Erdogan ; Arifiantini, Raden Iis ; Septian, Wike Andre ; Ardianto, Ari ; Pardede, Berlin Pandapotan ; Putri, Rafika Febriani

Molecular-based biomarkers are believed to be more accurate in determining bulls' fertility and sperm's various fertility characteristics. The sperm acrosome-associated 1 (SPACA1) molecule, located in the anterior acrosomal and equatorial segments of the sperm head, is thought to be related to the function of binding and fusion between sperm and oocyte. This study aims to analyze the association of SPACA1 mRNA and protein with the fertility rate and semen quality of Bali bulls (Bos sondaicus) and assess its potential as a potential molecular biomarker determining bull fertility and sperm quality. Frozen semen from 20 Bali bulls was used in the research, which was then divided into two groups: high (HF) and low fertility (LF). SPACA1 mRNA abundance was analyzed using qRT-PCR, and SPACA1 protein abundance was analyzed using EIA. The semen quality parameters analyzed were motility (CASA), plasma membrane integrity (HOS test), sperm head morphology abnormalities (William staining), sperm viability (SYBR 14-PI), sperm capacitation (CTC assay), and sperm acrosome integrity (FITC-PNA). The results showed that there was a significant difference (P < 0.05) between high fertility (HF) and low fertility (LF) bulls, both in the abundance of SPACA1 at the mRNA, protein, and semen quality levels. Overall, the correlation test results showed a close relationship (P < 0.01) between the abundance of SPACA1 mRNA and protein with field fertility and various semen quality parameters tested in the study. A low level of SPACA1 molecules indicates low fertility and semen quality in Bali bulls. The SPACA1 molecule has the potential to be developed through further research to become a biomarker for determining fertility and semen quality in bulls.

01 Oct 2024·Journal of Genetics and Genomics

Deficiency of MFSD6L, an acrosome membrane protein, causes oligoasthenoteratozoospermia in humans and mice

Article

Author: Zhou, Yiling ; Zhou, Xuehai ; Jin, Li ; Tang, Shuyan ; Zhou, Dapeng ; Wang, Jiaxiong ; Cao, Yunxia ; Liu, Chunyu ; He, Xiaojin ; Zhang, Feng ; Lv, Mingrong ; Wang, Lingbo ; Liu, Shuang ; Tian, Shixiong ; Shi, Huijuan ; Wang, Xuemei ; Wu, Huan ; Wu, Bangguo ; Tang, Jianan

Oligoasthenoteratozoospermia is an important factor affecting male fertility and has been found to be associated with genetic factors. However, there are still a proportion of oligoasthenoteratozoospermia cases that cannot be explained by known pathogenic genetic variants. Here, we perform genetic analyses and identify bi-allelic loss-of-function variants of MFSD6L from an oligoasthenoteratozoospermia-affected family. Mfsd6l knock-out male mice also present male subfertility with reduced sperm concentration, motility, and deformed acrosomes. Further mechanistic analyses reveal that MFSD6L, as an acrosome membrane protein, plays an important role in the formation of acrosome by interacting with the inner acrosomal membrane protein SPACA1. Moreover, poor embryonic development is consistently observed after intracytoplasmic sperm injection treatment using spermatozoa from the MFSD6L-deficient man and male mice. Collectively, our findings reveal that MFSD6L is required for the anchoring of sperm acrosome and head shaping. The deficiency of MFSD6L affects male fertility and causes oligoasthenoteratozoospermia in humans and mice.

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SPACA1

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