REV3L

Last update 08 May 2025

Basic Info

Synonyms

DNA polymerase zeta catalytic subunit, hREV3, POLZ

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Introduction

Catalytic subunit of the DNA polymerase zeta complex, an error-prone polymerase specialized in translesion DNA synthesis (TLS). Lacks an intrinsic 3'-5' exonuclease activity and thus has no proofreading function.

100 Clinical Results associated with REV3L

100 Translational Medicine associated with REV3L

0 Patents (Medical) associated with REV3L

386

Literatures (Medical) associated with REV3L

01 Apr 2025·Cell Reports

REV7 functions with REV3 as a checkpoint protein delaying mitotic entry until DNA replication is completed

Article

Author: Halazonetis, Thanos D ; Kohzaki, Masaoki ; Emamzadah, Soheila ; Hiller, Sebastian ; Mailler, Jonathan ; Huber, Florian ; Tropia, Laurence ; Sobkowiak, Katarzyna ; Böhm, Raphael

REV7, also named MAD2B or MAD2L2, is a subunit of the DNA translesion polymerase zeta and also part of the 53BP1-shieldin complex, which is present at sites of DNA double-strand breaks. REV7 has high sequence similarity to the MAD2 spindle assembly checkpoint protein, prompting us to examine whether REV7 has a checkpoint function. We observed that, in chicken and human cells exposed to agents that induce DNA replication stress, REV7 inhibits mitotic entry; this effect is most evident when the canonical DNA replication stress checkpoint, mediated by ATR, is inhibited. Similar to MAD2, REV7 undergoes conformational changes upon ligand binding, and its checkpoint function depends on its ability to homodimerize and bind its ligands. Notably, even in unchallenged cells, deletion of the REV7 gene leads to premature mitotic entry, raising the possibility that the REV7 checkpoint monitors ongoing DNA replication.

01 Jan 2025·Genes to Cells

“Chance and Necessity” on the Molecular Evolution of REV3 (a Catalytic Subunit of DNA Polymerase ζ)—The Dual Roles of Translesion and Neuronal Extension

Article

Author: Yamada, Kouichi ; Kajiwara, Kagemasa

ABSTRACTCatalytic subunit of DNA polymerase ζ (REV3), involved in translesion‐replication is evolutionarily conserved from yeast and plants to higher eukaryotes. However, a large intermediate domain is inserted in REV3 of humans and mice. The domain has “DUF4683” region, which is significantly similar to human neurite extension and migration factor (NEXMIF). This region was also found in REV3 of invertebrates such as Ciona intestinalis (sea squirt) … and Lingula anatina (Brachiopoda). We hypothesize foreign sequences were introduced into the Rev3 genes in the ancestral species of L. anatina, which would have gradually evolved into the DUF4683 region through overly complicated processes. Besides DUF4683, various exogenous sequences would have been inserted during the REV3 evolution. Therefore, insertion events of foreign sequences are all products of “necessity”. tBLASTn analysis of the Callorhinchus milii (elephant shark) genome with the C. milii REV3 sequence identified three neural factors (NEXMIF, NEXMIF‐like and AHDC1) in distinct positions of the genome. These factors may have differentiated from the Rev3 gene in Chondrichthyes, which had experienced two rounds of whole‐genome duplication, and may have evolved into neurite‐forming proteins in vertebrates. L. anatina has the DUF4683 C‐terminal proximal consensus (SPPRA/CWSP) in REV3. However, the consensus was not necessarily maintained in Mollusca, the closely related animal phylum of L. anatina. Just as written by Jacques Monod, we assume “chance” (mutation in DNA) and “necessity” (selection at the “organism” population level) were frequently repeated on the Rev3 in Cambrian ancestors. As a result, certain species developed the DUF4683 consensus of a neurite extension activity.

01 Jan 2025·International Journal of Medical Sciences

Identification of common diagnostic genes and molecular pathways in endometriosis and systemic lupus erythematosus by machine learning approach and in vitro experiment

Article

Author: Wang, Tao ; Liu, Wenwen ; Yang, Pusheng ; Miao, Yaxin ; Zhu, Yiping ; Zhang, Jiaxin ; Ge, Beilei ; Sun, Jing

Growing research suggests that endometriosis and systemic lupus erythematosus (SLE) are both chronic inflammatory diseases and closely related, but no studies have explored their common molecular characteristics and underlying mechanisms. Based on GEO datasets, differentially expressed genes in the endometriosis cohort and the SLE cohort were screened using Limma and weighted gene co-expression network analysis (WGCNA), and prediction signatures were constructed using LASSO logistic regression analysis, respectively. Four co-diagnostic genes (PMP22, QSOX1, REV3L, SP110) were identified for endometriosis and SLE. The nomogram, calibration curve, decision curve analyses (DCA), area under the receiver operating characteristic (AUC) curve and external datasets were used to evaluate the diagnostic and predictive value of co-diagnostic genes. The AUC value of the four co-diagnostic genes were higher than 0.85 in both endometriosis and SLE cohorts. Besides, functional enrichment analysis showed that DNA replication, base excision repair, cell cycle and cell adhesion molecules were significantly enriched. Multifactor regulatory network of four co-diagnostic genes was constructed including 96 TFs, 42 miRNA, 43 lncRNA, and 189 drugs, and Tributyrin was found to act on four co-diagnostic genes simultaneously. We identified and validated four co-diagnostic genes and revealed the potential molecular mechanisms of endometriosis and SLE, which is helpful for early diagnosis and targeted therapy. Our study provides a novel perspective for individualized treatment of patients with endometriosis and SLE.

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REV3L

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