Last update 01 Nov 2024

GMPR

Last update 01 Nov 2024

Basic Info

Synonyms

GMP reductase 1, GMPR, GMPR 1

+ [4]

Introduction

Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides.

100 Clinical Results associated with GMPR

100 Translational Medicine associated with GMPR

0 Patents (Medical) associated with GMPR

661

Literatures (Medical) associated with GMPR

01 Jan 2025·Colloids and Surfaces B: Biointerfaces

Ultrastable hierarchically porous nucleotide-based MOFs and their use for enzyme immobilization and catalysis

Article

Author: Zheng, Jiawei ; Ling, Jie ; Lin, Shuping ; Chen, Wenyi ; Zhong, Nanjing

Immobilization of free enzymes facilitates their recovery and reuse, while also enhances their enzymatic characteristics. Hierarchically porous metal-organic frameworks (HP-MOFs) are promising candidates for enzyme immobilization. However, fabrication of HP-MOFs with more kinds of components as ligands is still a challenge. Herein, ultrastable crystalline MOFs with micro-, meso- and macroporous structure were constructed using guanosine 5'-monophosphate (GMP) as organic ligand through templated emulsification method. HP-MOFs crystals with the near rhomb-like, rod-like and slab-like morphology were interestingly obtained from Zn²⁺, Cu²⁺ and Cd²⁺ respectively. The HP-MOFs immobilized enzymes exhibited an enhanced enzymatic activity and stability. In addition, the immobilized CALB (Candida antarctica lipase B) showed great glycerolysis and esterification performances for glycerides preparation, with diacylglycerols (DAG) content over 60 wt% and triacylglycerols (TAG) content over 90 wt% obtained respectively from glycerolysis and esterification. Moreover, it retained 82.32 % of its initial glycerolysis activity after six cycles of reuse in glycerolysis. The present study will provide clues and show new horizons to explore new organic ligands for HP-MOFs fabrication, as well as to expand the applications of HP-MOFs and their supported enzymes.

01 Oct 2024·Acta Crystallographica Section F Structural Biology Communications

Crystal structure of guanosine 5′-monophosphate synthetase from the thermophilic bacterium Thermus thermophilus HB8

Article

Author: Sampei, Gen Ichi ; Baba, Seiki ; Nemoto, Naoki ; Kawai, Gota

Guanosine 5′-monophosphate (GMP) synthetase (GuaA) catalyzes the last step of GMP synthesis in the purine nucleotide biosynthetic pathway. This enzyme catalyzes a reaction in which xanthine 5′-monophosphate (XMP) is converted to GMP in the presence of Gln and ATP through an adenyl-XMP intermediate. A structure of an XMP-bound form of GuaA from the domain Bacteria has not yet been determined. In this study, the crystal structure of an XMP-bound form of GuaA from the thermophilic bacterium Thermus thermophilus HB8 (TtGuaA) was determined at a resolution of 2.20 Å and that of an apo form of TtGuaA was determined at 2.10 Å resolution. TtGuaA forms a homodimer, and the monomer is composed of three domains, which is a typical structure for GuaA. Disordered regions in the crystal structure were obtained from the AlphaFold2-predicted model structure, and a model with substrates (Gln, XMP and ATP) was constructed for molecular-dynamics (MD) simulations. The structural fluctuations of the TtGuaA dimer as well as the interactions between the active-site residues were analyzed by MD simulations.

19 Sep 2024·The journal of sexual medicine

Chronic sleep deprivation induces erectile dysfunction through increased oxidative stress, apoptosis, endothelial dysfunction, and corporal fibrosis in a rat model.

Article

Author: Wu, Xu ; Liu, Guodong ; Zhang, Yuyang ; Feng, Xingliang ; Su, Mingqin ; Zhang, Xiansheng ; Jiang, Hui ; Tang, Dongdong

BACKGROUNDSleep is foundational for nocturnal erections, facilitating nutrient exchange and waste removal, which has brought widespread attention to the relationship between sleep and erectile dysfunction (ED). However, there is currently a lack of basic research confirming whether chronic sleep deprivation (CSD) leads to erectile impairment and its underlying pathological mechanisms.AIMThe study sought to investigate whether CSD impairs erectile function in rats and the potential tissue damage it may cause in rats.METHODSThe modified multiple platform method was employed to induce CSD in 14 rats, randomly divided into a platform control group and a CSD group. After 3 weeks, erectile function was evaluated by measuring intracavernosal pressure following cavernous nerve stimulation.OUTCOMESArterial blood samples were then analyzed for testosterone levels, and cavernous tissues were processed for advanced molecular biology assays, including Western blotting and immunofluorescence.RESULTSAfter inducing CSD, rats exhibited a marked reduction in erectile function, yet their serum testosterone levels remained statistically unchanged when compared with the control group. More importantly, rats in the CSD group exhibited a significant increase in oxidative stress levels, accompanied by low expression of HO-1 and high expression of NOX1 and NOX4. Subsequently, elevated oxidative stress induced increased apoptosis in smooth muscle and endothelial cells, as evidenced by significant decreases in CD31 and α-smooth muscle actin expression in the CSD group, demonstrated through Western blotting and immunofluorescence assays. Endothelial cell apoptosis led to a significant decrease in endothelial nitric oxide synthase, resulting in lowered levels of nitric oxide and cyclic guanosine monophosphate, which severely impaired the erectile mechanism. Additionally, activation of the transforming growth factor β1 fibrotic pathway led to increased levels of tissue fibrosis, resulting in irreversible damage to the penile tissue in the CSD group.CLINICAL IMPLICATIONSOur study lacks further exploration of the molecular mechanisms linking CSD and ED, representing a future research focus for potential targeted therapies.STRENGTHS AND LIMITATIONSOur findings demonstrated that CSD significantly impairs erectile function in rats.CONCLUSIONCSD severely impairs erectile function in rats. When exposed to CSD, rats exhibit significantly elevated oxidative stress levels, which lead to increased tissue apoptosis, endothelial dysfunction, and ultimately irreversible fibrotic changes in the tissues. Further researches into the potential molecular mechanisms are needed to identify possible therapeutic targets for ED related to CSD.

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