CHML

Last update 08 May 2025

Basic Info

Synonyms

CHM like Rab escort protein, CHML, Choroideremia-like protein

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Introduction

Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family.

100 Clinical Results associated with CHML

100 Translational Medicine associated with CHML

0 Patents (Medical) associated with CHML

146

Literatures (Medical) associated with CHML

01 Mar 2024·Journal of Global Antimicrobial Resistance

Outbreak of vancomycin-resistant Enterococcus faecium ST1133 in paediatric patients with acute lymphoblastic leukaemia from southern Brazil

Article

Author: Medeiros Dos Santos, Érika ; Krul, Damaris ; Conte, Danieli ; Siqueira, Adriele Celine ; Mesa, Dany ; de Abreu, Renata Barbosa Vahia ; Vasconcelos, Thaís Muniz ; Rodrigues, Luiza Souza ; Motta, Fábio de Araújo ; Dalla-Costa, Libera Maria

OBJECTIVESWe aimed to investigate an outbreak of vancomycin-resistant Enterococcus faecium (VREfm) in paediatric patients from Hospital Pequeno Príncipe. The susceptibility profile was determined, and whole-genome sequencing (WGS) was used to analyse the genetic context of the strains.METHODSFive VREfm isolates were recovered from sterile sites and surveillance cultures of two paediatric patients with acute lymphoblastic leukaemia. Species identification was performed using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and the minimum inhibitory concentration (MIC) was assessed according to the European Committee for Antimicrobial Susceptibility Testing (EUCAST). WGS was performed to analyse the genetic context of virulence and resistance genes, and in silico multilocus sequence typing was performed to identify the sequence typing of the strains.RESULTSHigh-level vancomycin resistance was observed in all isolates (≥256 mg/L). WGS revealed the presence of mobile genetic elements, such as plasmids (rep₂, rep_11a, rep_US15, rep₁₇, and rep_18a), insertion sequences, and phages. Multiple resistance genes (aac(6')-aph(2"), dfrG, ermB, and vanA) and virulence genes (acm and efaAfm) were identified. All the isolates were assigned to ST117 (ST1133 - via a novel MLST), an important epidemic lineage associated with nosocomial infections and outbreaks.CONCLUSIONOur results show that the ST117 (ST1133) VREfm isolates are circulating in paediatric patients, which raises a great concern. The development of new drugs as well as the implementation of an antimicrobial stewardship program are necessary for their correct management, limiting the spread of resistance in oncohematological patients.

21 Feb 2024·Applied and Environmental Microbiology

Effects of Neolamarckia cadamba leaves extract on microbial community and antibiotic resistance genes in cecal contents and feces of broilers challenged with lipopolysaccharides

Article

Author: Hu, Lei ; Zhou, Wei ; Zhang, Qing ; Wu, Shuo ; Wang, Cheng ; Cao, Yong ; Chen, Xiaoyang ; Wang, Li ; Hu, Qi

ABSTRACT The effects of Neolamarckia cadamba leaves extract (NCLE), with effective ingredients of flavonoids, on antibiotic resistance genes (ARGs) and relevant microorganisms in cecal contents and feces of broilers treated with or without lipopolysaccharide stimulation (LPS) were investigated. LPS stimulation increased ( P < 0.05) the relative abundance of ARGs and mobile genetic elements (MGEs), such as tet(W/N/W ), APH(3')-IIIa , ErmB , tet (44 ), ANT (6)-Ia , tet(O ), tet (32 ), Vang_ACT_CHL , myrA , ANT (6)-Ib , IncQ1 , tniB , and rep2 in cecal contents. However, the difference disappeared ( P > 0.05) when NCLE was added at the same time. These differential ARGs and MGEs were mainly correlated ( P < 0.01) with Clostridiales bacterium, Lachnospiraceae bacterium, and Candidatus Woodwardibium gallinarum . These species increased in LPS-stimulated broilers and decreased when NCLE was applied at the same time. In feces, LPS stimulation decreased ( P < 0.05) the relative abundance of tet(Q ), adeF , ErmF , Mef(En2 ), OXA-347 , tet (40 ), npmA , tmrB, CfxA3, and ISCrsp1, while the LPS + NCLE treated group showed no significant effect ( P > 0.05) on these ARGs. These differential ARGs and MGEs in feces were mainly correlated ( P < 0.01) with Clostridiales bacterium, Pseudoflavonifractor sp . An184, Flavonifractor sp . An10, Ruminococcaceae bacterium, etc. These species increased in LPS-stimulated broilers and increased when NCLE was applied at the same time. In conclusion, LPS stimulation and NCLE influenced microbial communities and associated ARGs in both cecal contents and feces of broilers. NCLE alleviated the change of ARGs and MGEs in LPS-induced broilers by maintaining the microbial balance. IMPORTANCEAntibiotics showed a positive effect on gut health regulation and growth performance improvement in livestock breeding, but the antimicrobial resistance threat and environment pollution problem are increasingly severe with antibiotics abuse. As alternatives, plant extract containing bioactive substances are increasingly used to improve immunity and promote productivity. However, little is known about their effects on diversity and abundance of ARGs. Here, we investigated the effects of NCLE, with effective ingredients of flavonoids, on ARGs and relevant microorganisms in cecal contents and feces of broilers treated with or without lipopolysaccharide stimulation. We found that NCLE reduced the abundance of ARGs in cecal contents of lipopolysaccharide-induced broilers by maintaining the microbial balance. This study provides a comprehensive view of cecal and fecal microbial community, ARGs, and MGEs of broiler following LPS stimulation and NCLE treatment. It might be used to understand and control ARGs dissemination in livestock production.

01 Jan 2024·Methods in Molecular Biology

Recombinant AAV Production

Article

Author: Venereo-Sanchez, Alina ; Joshi, Pranav R H

The insect cell-baculovirus expression vector (IC-BEV) platform has enabled small research-scale and large commercial-scale production of recombinant proteins and therapeutic biologics including recombinant adeno-associated virus (rAAV)-based gene delivery vectors. The wide use of this platform is comparable with other mammalian cell line-based platforms due to its simplicity, high-yield, comparable quality attributes, and robust bioprocessing features. In this chapter, we describe a rAAV production protocol employing one of the recent modifications of the One-Bac platform that consists of a stable transformed Sf9 cell line carrying AAV Rep2/Cap5 genes that are induced upon infection with a single recombinant baculovirus expression vector harboring the transgene of interest (rAAV genome). The overall protocol consists of essential steps including rBEV working stock preparation, rAAV production, and centrifugation-based clarification of cell culture lysate. The same protocol can also be applied for rAAV vector production using traditional Three-Bac, Two-Bac, and Mono-Bac platforms without requiring significant changes.

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