PCTP

Last update 08 May 2025

Basic Info

Synonyms

PC-TP, PCTP, phosphatidylcholine transfer protein

+ [3]

Introduction

Catalyzes the transfer of phosphatidylcholine between membranes. Binds a single lipid molecule.

100 Clinical Results associated with PCTP

100 Translational Medicine associated with PCTP

0 Patents (Medical) associated with PCTP

124

Literatures (Medical) associated with PCTP

01 Nov 2024·Journal of Biological Chemistry

Activity and phosphatidylcholine transfer protein interactions of skeletal muscle thioesterase Them2 enable hepatic steatosis and insulin resistance

Article

Author: Cohen, David E. ; Xie, Yang ; Ortlund, Eric A. ; Liu, Wenpeng ; Liu, Xu ; Carr, Logan R ; Cohen, David E ; Lee, Luke P ; Ortlund, Eric A ; Lee, Luke P. ; Imai, Norihiro ; Carr, Logan R.

Thioesterase superfamily member 2 (Them2), a long-chain fatty acyl-CoA thioesterase that is highly expressed in oxidative tissues, interacts with phosphatidylcholine transfer protein (PC-TP) to regulate hepatic lipid and glucose metabolism and to suppress insulin signaling. High-fat diet-fed mice lacking Them2 globally or specifically in skeletal muscle, but not liver, exhibit reduced hepatic steatosis and insulin resistance. Here, we report that the capacity of Them2 in skeletal muscle to promote hepatic steatosis and insulin resistance depends on both its catalytic activity and interaction with PC-TP. Two residues of Them2 catalytic site were mutated (N50A/D65A) to produce the inactive enzyme while maintaining its homotetrameric structure and interaction with PC-TP. Restoration of skeletal muscle expression in Them2^-/- mice using recombinant adeno-associated virus revealed that WT, but not N50A/D65A Them2, promoted high-fat diet-induced weight gain and hepatic steatosis. This was accompanied by greater impairment of insulin sensitivity in WT than N50A/D65A Them2. Pharmacological inhibition or genetic ablation of PC-TP attenuated these effects. In reductionist experiments, conditioned medium collected from WT primary cultured myotubes promoted excess lipid accumulation in oleic acid-treated primary cultured hepatocytes relative to Them2^-/- myotubes, which was attributable to secreted extracellular vesicles. Reconstitution of Them2 expression in Them2^-/- myotubes affirmed the requirements for catalytic activity and PC-TP interactions for extracellular vesicles to promote lipid accumulation in hepatocytes. These studies provide valuable mechanistic insights, whereby Them2 in skeletal muscle promotes hepatic steatosis and establish both Them2 and PC-TP as attractive targets for managing metabolic dysfunction-associated steatotic liver disease.

15 Aug 2024·The Journal of Physical Chemistry Letters

Model Mechanism for Lipid Uptake by the Human STARD2/PC-TP Phosphatidylcholine Transfer Protein

Article

Author: Talandashti, Reza ; Moqadam, Mahmoud ; Reuter, Nathalie

The human StAR-related lipid transfer domain protein 2 (STARD2), also known as phosphatidylcholine (PC) transfer protein, is a single-domain lipid transfer protein thought to transfer PC lipids between intracellular membranes. We performed extensive μs-long molecular dynamics simulations of STARD2 of its apo and holo forms in the presence or absence of complex lipid bilayers. The simulations in water reveal ligand-dependent conformational changes. In the 2 μs-long simulations of apo STARD2 in the presence of a lipid bilayer, we observed spontaneous reproducible PC lipid uptake into the protein hydrophobic cavity. We propose that the lipid extraction mechanism involves one to two metastable states stabilized by choline-tyrosine or choline-tryptophane cation-π interactions. Using free energy perturbation, we evaluate that PC-tyrosine cation-π interactions contribute 1.8 and 2.5 kcal/mol to the affinity of a PC-STARD2 metastable state, thus potentially providing a significant decrease of the energy barrier required for lipid desorption.

01 Jan 2024·Biology of Sport

Genetic markers and predictive model for individual differences in countermovement jump enhancement after resistance training

Article

Author: He, Zihong ; Li, Yanchun ; Li, Xiaoxia ; Yang, Xiaolin ; Li, Liang ; Mei, Tao

This study aims to utilize Genome-Wide Association Analysis (GWAS) to identify genetic markers associated with enhanced power resulting from resistance training. Additionally, we analyze the potential biological effects of these markers and establish a predictive model for training outcomes. 193 Han Chinese adults (age: 20 ± 1 years) underwent resistance training involving squats and bench presses at 70% 1RM, twice weekly, 5 sets × 10 repetitions, for 12 weeks. Whole-genome genotyping was conducted, and participants' countermovement jump (CMJ) height, lower limb muscle strength, and body muscle mass were assessed. CMJ height change was used to assess changes in power and subjected to Genome-Wide Association Analysis (GWAS) against genotypes. Employing Polygenic Score (PGS) calculations and stepwise linear regression, a predictive model for training effects was constructed. The results revealed a significant increase in CMJ height among participants following the resistance training intervention (Δ% = 16.53%, p < 0.01), with individual differences ranging from -35.90% to 125.71%. 38 lead SNPs, including PCTP rs9907859 (p < 1 × 10^-8), showed significant associations with the percentage change in CMJ height after training (p < 1 × 10^-5). The explanatory power of the predictive model for training outcomes, established using PGS and phenotypic indicators, was 62.6%, comprising 13.0% from PGS and 49.6% from phenotypic indicators. SNPs associated with power resistance training were found to participate in the biological processes of musculoskeletal movement and the Striated muscle contraction pathway. These findings indicate that individual differences in the training effect of CMJ exist after resistance training, partially explained by genetic markers and phenotypic indicators (62.6%).

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PCTP

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