ABSTRACTLeishmaniasis is a neglected tropical disease, caused by protozoan parasites of Leishmania (L.), and is transmitted by bite of phlebotomine sandflies. There are several studies on central nervous system infection to indicate that Leishmania can cross the blood–brain barrier, resulting in neurological manifestations, known as “cerebral leishmaniasis.” This study highlighted the notions: (i) polarisation of bone marrow‐derived macrophages (BMDM) incubated following stimulation with lipopolysaccharide (LPS) or soluble Leishmania antigen (SLA), (ii) quantification of parasites within co‐culture of Leishmania‐infected macrophages, and astrocytes, and (iii) effect of interferon‐gamma (IFN‐γ) on the infection rate of co‐culture populations. Accordingly, 83% of overall macrophage population was identified on day 7 for CD11b and F4/80 macrophage markers. Flow cytometry analysis revealed significant increases in CD11b and F4/80 surface markers in LPS and SLA‐stimulated BMDMs at 24 h, compared to untreated cells. TNF‐α levels increased significantly in both LPS and SLA‐treated BMDMs after 48 h. Additionally, SLA treatment induced a more elongated, spindle‐like shape in the cells, indicative of M2 macrophage polarisation over the M1 phenotype. When non‐infected astrocytes with/without stimulation with IFN‐γ before co‐culture, gp63 FITC‐labelled parasite populations (%) in co‐culture decreased to 25% at 72 h, thus indicating a lower infection rate in a time‐dependent manner. IFN‐γ and IL‐6 levels significantly increased to 71.66 ± 3.51 and 184 ± 14.42 pg/mL, resulting in the inflammatory response in the co‐culture system at 48 h (p ≤ 0.0001), when compared to the control (30 ± 2.52 pg/mL for IFN‐γ and 8.66 ± 2.37 pg/mL for IL‐6) at 0 h of the incubation. It is the first study to emphasize the communication between Leishmania‐infected macrophages and astrocytes regarding Leishmania parasite load. The results suggest that astrocytes can lead to the reduction in Leishmania parasites, thereby controlling the incidence of cerebral leishmaniasis.