ASAP3

Last update 08 May 2025

Basic Info

Synonyms

Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3, ArfGAP with SH3 domain, ankyrin repeat and PH domain 3, ASAP3

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Introduction

Promotes cell proliferation.

100 Clinical Results associated with ASAP3

100 Translational Medicine associated with ASAP3

0 Patents (Medical) associated with ASAP3

Literatures (Medical) associated with ASAP3

05 Jun 2024·Neurophotonics

Two-dimensional electro-optical multiphoton microscopy

Article

Author: Kerlin, Aaron ; Jayakumar, Harishankar ; Leger, James ; Gable, Jacob ; Newman, Zachary L. ; Farinella, Deano M. ; Stanek, Samuel

SignificanceThe development of genetically encoded fluorescent indicators of neural activity with millisecond dynamics has generated demand for ever faster two-photon (2P) imaging systems, but acoustic and mechanical beam scanning technologies are approaching fundamental limits. We demonstrate that potassium tantalate niobate (KTN) electro-optical deflectors (EODs), which are not subject to the same fundamental limits, are capable of ultrafast two-dimensional (2D) 2P imaging in vivo.AimTo determine if KTN-EODs are suitable for 2P imaging, compatible with 2D scanning, and capable of ultrafast in vivo imaging of genetically encoded indicators with millisecond dynamics.ApproachThe performance of a commercially available KTN-EOD was characterized across a range of drive frequencies and laser parameters relevant to in vivo 2P microscopy. A second KTN-EOD was incorporated into a dual-axis scan module, and the system was validated by imaging signals in vivo from ASAP3, a genetically encoded voltage indicator.ResultsOptimal KTN-EOD deflection of laser light with a central wavelength of 960 nm was obtained up to the highest average powers and pulse intensities tested (power: 350 mW; pulse duration: 118 fs). Up to 32 resolvable spots per line at a 560 kHz line scan rate could be obtained with single-axis deflection. The complete dual-axis EO 2P microscope was capable of imaging a __-mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"-__13 μm by __-mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"-__13 μm field-of-view at over 10 kHz frame rate with __-mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"-__∼0.5 μm lateral resolution. We demonstrate in vivo imaging of neurons expressing ASAP3 with high temporal resolution.ConclusionsWe demonstrate the suitability of KTN-EODs for ultrafast 2P cellular imaging in vivo, providing a foundation for future high-performance microscopes to incorporate emerging advances in KTN-based scanning technology.

01 May 2024·Advanced Science

An Ultrasensitive Genetically Encoded Voltage Indicator Uncovers the Electrical Activity of Non‐Excitable Cells

Article

Author: Nair, Anagha G. ; Münster, Lukas ; Rühl, Philipp ; Gawande, Namrata ; Schönherr, Roland ; Dehiwalage, Sassrika N. C. W. ; Heinemann, Stefan H.

AbstractMost animal cell types are classified as non‐excitable because they do not generate action potentials observed in excitable cells, such as neurons and muscle cells. Thus, resolving voltage signals in non‐excitable cells demands sensors with exceptionally high voltage sensitivity. In this study, the ultrabright, ultrasensitive, and calibratable genetically encoded voltage sensor rEstus is developed using structure‐guided engineering. rEstus is most sensitive in the resting voltage range of non‐excitable cells and offers a 3.6‐fold improvement in brightness change for fast voltage spikes over its precursor ASAP3. Using rEstus, it is uncovered that the membrane voltage in several non‐excitable cell lines (A375, HEK293T, MCF7) undergoes spontaneous endogenous alterations on a second to millisecond timescale. Correlation analysis of these optically recorded voltage alterations provides a direct, real‐time readout of electrical cell–cell coupling, showing that visually connected A375 and HEK293T cells are also largely electrically connected, while MCF7 cells are only weakly coupled. The presented work provides enhanced tools and methods for non‐invasive voltage imaging in living cells and demonstrates that spontaneous endogenous membrane voltage alterations are not limited to excitable cells but also occur in a variety of non‐excitable cell types.

01 Jul 2023·Nature Methods

A positively tuned voltage indicator for extended electrical recordings in the brain

Article

Author: Madruga, Blake ; Pradhan, Lagnajeet ; Hao, Yukun A ; Negrean, Adrian ; Ji, Na ; Dror, Ron O ; Pang, Michelle M ; Zhang, Guofeng ; Ding, Jun B ; Dorian, Conor C ; Taxidis, Jiannis ; Reese, Austin L ; Giocomo, Lisa M ; Roth, Richard H ; Fan, Jiang Lan ; Su, Sharon ; Makinson, Christopher D ; Plitt, Mark H ; Losonczy, Attila ; Shi, Dong-Qing ; Lin, Michael Z ; Golshani, Peyman ; Evans, S Wenceslao ; Lee, Sungmoo ; Chavarha, Mariya ; Clandinin, Thomas R ; Hwang, Fuu-Jiun ; Wang, Sui ; van Keulen, Siri C ; Suomivuori, Carl-Mikael ; Jiang, Dongyun ; Liu, Yu ; Bi, Guo-Qiang

Genetically encoded voltage indicators (GEVIs) enable optical recording of electrical signals in the brain, providing subthreshold sensitivity and temporal resolution not possible with calcium indicators. However, one- and two-photon voltage imaging over prolonged periods with the same GEVI has not yet been demonstrated. Here, we report engineering of ASAP family GEVIs to enhance photostability by inversion of the fluorescence-voltage relationship. Two of the resulting GEVIs, ASAP4b and ASAP4e, respond to 100-mV depolarizations with ≥180% fluorescence increases, compared with the 50% fluorescence decrease of the parental ASAP3. With standard microscopy equipment, ASAP4e enables single-trial detection of spikes in mice over the course of minutes. Unlike GEVIs previously used for one-photon voltage recordings, ASAP4b and ASAP4e also perform well under two-photon illumination. By imaging voltage and calcium simultaneously, we show that ASAP4b and ASAP4e can identify place cells and detect voltage spikes with better temporal resolution than commonly used calcium indicators. Thus, ASAP4b and ASAP4e extend the capabilities of voltage imaging to standard one- and two-photon microscopes while improving the duration of voltage recordings.

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ASAP3

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