STX5

Last update 08 May 2025

Basic Info

Synonyms

SED5, STX5, STX5A

+ [2]

Introduction

Mediates endoplasmic reticulum to Golgi transport. Together with p115/USO1 and GM130/GOLGA2, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. (Microbial infection) Required for the efficient production of infectious virion during human cytomegalovirus infection. Mechanistically, participates in the formation of the cytoplasmic viral assembly compartment where tegument acquisition and envelopment occur. Required for Golgi to endoplasmic reticulum retrogade transport, and for intra-Golgi transport.

100 Clinical Results associated with STX5

100 Translational Medicine associated with STX5

0 Patents (Medical) associated with STX5

198

Literatures (Medical) associated with STX5

01 Mar 2025·Pesticide Biochemistry and Physiology

Transcriptomic insights on impaired survival and enhanced pesticide susceptibility following knockdown of Syntaxin5 in Locusta migratoria

Article

Author: Krishnan, Natraj ; Keyhani, Nemat O ; Chen, Xuanyu ; Zang, Lian-Sheng ; Zhang, Wei ; Wang, Guangmin ; Paredes-Montero, Jorge R ; Ali, Asad ; Mohamed, Amr

Membrane fusion, essential for a variety of biological processes, is ubiquitous and critical in all living organisms. The soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) comprise the core machinery that mediates membrane fusion. Syntaxin5 (Stx5) in mammals and its ortholog Sed5p in yeast are SNARE proteins that have been reported to mediate anterograde and retrograde endoplasmic reticulum (ER)-Golgi trafficking. In this study, a syntaxin5 gene (Lmsyx5) was cloned from the migratory locust, Locusta migratoria, and its tissue expression pattern showed widespread expression in different tissues. Knockdown of this gene using RNA interference (RNAi) revealed that both 5^th instars and adult locusts have reduced survival, and almost all of the 5^th instar locusts died before molting. Transcriptomic analysis revealed that drug or xenobiotic metabolism-related pathways are the most significantly downregulated in the central nervous system (CNS) of the RNAi 5^th instar locusts, whereas amino acid metabolism-related pathways are significantly downregulated in the muscle. In addition, receptor interaction-related pathways are downregulated in both the CNS and muscle. The mortality rate was significantly higher when dsLmsyx5 and beta-cypermethrin were combined together than when RNAi and chemical pesticides were used alone. Taken together, the Lmsyx5 gene is essential for locust survival, affects locust molting, is involved in the metabolism of both xenobiotics and endogenous chemicals in various tissues, and regulates locust susceptibility to chemical pesticides. It can thus be a potential target for locust control, and its knockdown using RNAi has great potential when combined with chemical pesticides.

03 Jun 2024·Journal of Cell Biology

SM protein Sly1 and a SNARE Habc domain promote membrane fusion through multiple mechanisms

Article

Author: Lin, Ariel ; Merz, Alexey J. ; Gao, Guanbin ; Duan, Mengtong ; Banfield, David K. ; Mackey, Emma J.

SM proteins including Sly1 are essential cofactors of SNARE-mediated membrane fusion. Using SNARE and Sly1 mutants and chemically defined in vitro assays, we separate and assess proposed mechanisms through which Sly1 augments fusion: (i) opening the closed conformation of the Qa-SNARE Sed5; (ii) close-range tethering of vesicles to target organelles, mediated by the Sly1-specific regulatory loop; and (iii) nucleation of productive trans-SNARE complexes. We show that all three mechanisms are important and operate in parallel, and that close-range tethering promotes trans-complex assembly when cis-SNARE assembly is a competing process. Further, we demonstrate that the autoinhibitory N-terminal Habc domain of Sed5 has at least two positive activities: it is needed for correct Sed5 localization, and it directly promotes Sly1-dependent fusion. “Split Sed5,” with Habc presented solely as a soluble fragment, can function both in vitro and in vivo. Habc appears to facilitate events leading to lipid mixing rather than promoting opening or stability of the fusion pore.

01 Jun 2024·Pesticide Biochemistry and Physiology

Syntaxin5 is essential for survival by ensuring midgut epithelial homeostsis and regulating feeding in Locusta migratoria

Article

Author: Zhang, Jianzhen ; Liu, Xiaojian ; Gao, Ya ; Li, Yao ; El Wakil, Abeer ; Moussian, Bernard

Syntaxin5 (Syx5) belongs to SNAREs family, which play important roles in fusion of vesicles to target membranes. Most of what we know about functions of Syx5 originates from studies in fungal or vertebrate cells, how Syx5 operates during the development of insects is poorly understood. In this study, we investigated the role of LmSyx5 in the gut development of the hemimetabolous insect Locusta migratoria. LmSyx5 was expressed in many tissues, with higher levels in the gut. Knockdown of LmSyx5 by RNA interference (RNAi) considerably suppressed feeding in both nymphs and adults. The dsLmSyx5-injected locusts lost body weight and finally died at a mortality of 100%. Furthermore, hematoxylin-eosin staining indicated that the midgut is deformed in dsLmSyx5-treated nymphs and the brush border in midgut epithelial cells is severely damaged, suggesting that LmSyx5 is involved in morphogenesis of the midgut. TEM further showed that the endoplasmic reticulum of midgut cells have a bloated appearance. Taken together, these results suggest that LmSyx5 is essential for midgut epithelial homeostsis that affects growth and development of L. migratoria. Thus, Syx5 is a promising RNAi target for controlling L. migratoria, and even other pests.

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