MIR155HG

Last update 23 Jan 2025

Basic Info

Synonyms

BIC, miPEP155, MIR155 host gene

+ [3]

Introduction-

100 Clinical Results associated with MIR155HG

100 Translational Medicine associated with MIR155HG

0 Patents (Medical) associated with MIR155HG

284

Literatures (Medical) associated with MIR155HG

01 Apr 2025·Archives of Medical Research

Deficiency of miR-155 in leukemic B-cells results in cell cycle arrest and deregulation of MIR155HG/TP53INP1/CDKN1A/CCND1 network

Article

Author: Sebela, Marek ; Sefc, Ludek ; Vargova, Karina Savvulidi ; Kokavec, Juraj ; Stopka, Tomas ; Bajecny, Martin ; Tost, Jorg ; Kazantsev, Dmitry ; Savvulidi, Filipp Georgijevic ; Herynek, Vit ; Yurikova, Oxana ; Lenobel, Rene ; Zemanova, Zuzana ; Golovina, Elena ; Klener, Pavel ; Simersky, Radim

BACKGROUNDCell cycle progression and leukemia development are tightly regulated processes in which even a small imbalance in the expression of cell cycle regulatory molecules and microRNAs (miRNAs) can lead to an increased risk of cancer/leukemia development. Here, we focus on the study of a ubiquitous, multifunctional, and oncogenic miRNA-hsa-miR-155-5p (miR-155, MIR155HG), which is overexpressed in malignancies including chronic lymphocytic leukemia (CLL). Nonetheless, the precise mechanism of how miR-155 regulates the cell cycle in leukemic cells remains the subject of extensive research.METHODSWe edited the CLL cell line MEC-1 by CRISPR/Cas9 to introduce a short deletion within the MIR155HG gene. To describe changes at the transcriptome and miRNome level in miR-155-deficient cells, we performed mRNA-seq/miRNA-seq and validated changes by qRT-PCR. Flow cytometry was used to measure cell cycle kinetics. A WST-1 assay, hemocytometer, and Annexin V/PI staining assessed cell viability and proliferation.RESULTSThe limited but phenotypically robust miR-155 modification impaired cell proliferation, cell cycle, and cell ploidy. This was accompanied by overexpression of the negative cell cycle regulator p21/CDKN1A and Cyclin D1 (CCND1). We confirmed the overexpression of canonical miR-155 targets such as PU.1, FOS, SHIP-1, TP53INP1 and revealed new potential targets (FCRL5, ISG15, and MX1).CONCLUSIONSWe demonstrate that miR-155 deficiency impairs cell proliferation, cell cycle, transcriptome, and miRNome via deregulation of the MIR155HG/TP53INP1/CDKN1A/CCND1 axis. Our CLL model is valuable for further studies to manipulate miRNA levels to revert highly aggressive leukemic cells to nearly benign or non-leukemic types.

01 Jan 2025·Clinical Oral Implants Research

Hyperbranched Poly‐l‐Lysine Modified Titanium Surface With Enhanced Osseointegration, Bacteriostasis, and Anti‐Inflammatory Properties for Implant Application: An Experimental In Vivo Study

Article

Author: Miao, Xiaoyan ; Wang, Zhikang ; Jiang, Qifeng ; Chen, Chaozhen ; Gao, Changyou ; Dai, Wei ; Yang, Guoli ; Qin, Xiaoru ; Zhang, Yanmin ; Jiang, Zhiwei ; Xi, Yue ; Wang, Zhaolong

ABSTRACTObjectivesThis study aimed to explore multiple effects of hyperbranched poly‐l‐lysine (HBPL) titanium (Ti) surfaces on osseointegration, bacteriostasis, and anti‐inflammation across three different animal models.MethodsTi surfaces were covalently modified with HBPL, with uncoated surfaces as controls. Characterization included scanning electron microscopy (SEM) and surface chemistry and elemental analysis (EDX). Ti and Ti‐HBPL implants were placed in conventional canine edentulous sites, post‐operative infection canine edentulous sites, and diabetic rat tibias. Implants from canine edentulous models were analyzed using micro‐CT and histomorphometry to assess osseointegration at 8 weeks. Post‐operative infection beagles were used to evaluate antibacterial efficacy through clinical parameters and bacterial cultures at 1 week. In diabetic rats, micro‐CT and histomorphometry were performed at 8 weeks.ResultsHBPL was uniformly grafted on Ti‐HBPL surfaces. Ti‐HBPL surfaces showed higher bone volume/total volume (BV/TV, p < 0.001), bone‐implant contact (BIC%, p < 0.001), and trabecular number (Tb.N, p < 0.01) in beagles. Besides, it displayed higher BIC% (p < 0.001) and bone area fraction occupancy (BAFO%, p < 0.01) in hard tissue sections. In an infected model, Ti‐HBPL surfaces exhibited lower bleeding on probing (BOP, p < 0.001), and plaque index (DI, p < 0.01), with reduced bacterial colony formation (p < 0.001) compared to the control group. In diabetic rats, Ti‐HBPL surfaces showed an increase in BV/TV (p < 0.01) and Tb.N (p < 0.001), downregulated TNF‐α and IL‐1β (p < 0.01), and upregulated IL‐10 (p < 0.01) and osteocalcin (OCN) expression (p < 0.01).ConclusionsHBPL‐Ti surfaces demonstrated enhanced osseointegration, bacteriostasis, and anti‐inflammatory effects in vivo.

30 Oct 2024·Bioscience Reports

Expression of Concern: LncRNA MIR155HG contributes to smoke-related chronic obstructive pulmonary disease by targeting miR-128-5p/BRD4 axis

Article

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MIR155HG

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