NANOS3

Last update 23 Jan 2025

Basic Info

Synonyms

nanos C2HC-type zinc finger 3, Nanos homolog 3, NANOS1L

+ [4]

Introduction

Plays a role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Affects cell proliferation probably by repressing translation of specific mRNAs. Maintains the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways. Essential in the early stage embryo to protect the migrating primordial germ cells (PGCs) from apoptosis.

100 Clinical Results associated with NANOS3

100 Translational Medicine associated with NANOS3

0 Patents (Medical) associated with NANOS3

176

Literatures (Medical) associated with NANOS3

01 Feb 2025·Poultry Science

High levels of histone acetylation modifications promote the formation of PGCs

Article

Author: Geng, Qingqing ; Cheng, Fufu ; Zuo, Qisheng ; Zhao, Ziduo ; Zhang, Yani ; Wu, Yuhui ; Niu, Yingjie ; Wang, Zhe

This study investigates the role of histone acetylation in the differentiation of chicken embryonic stem cells (ESCs) into primordial germ cells (PGCs). Transcriptomic sequencing was used to analyze differentially expressed genes during this differentiation process, with functional annotation identifying genes associated with histone acetylation. To explore the role of acetylation, acetate and an acetyltransferase inhibitor (ANAC) were added to the ESCs induction medium. Transcriptomic analysis revealed that during ESCs differentiation into PGCs, genes involved in histone acetyltransferase activity were upregulated, while those associated with histone deacetylase activity were downregulated. Functional enrichment analysis indicated these genes are involved in pathways critical for germ cell differentiation, underscoring their importance in avian reproductive biology. Quantitative real-time PCR (qRT-PCR) confirmed significant differential expression of HAT8 and HDAC10 between ESCs and PGCs (P < 0.01). The acetate treatment group exhibited a significantly higher number of embryoid bodies and elevated expression levels of CVH, C-KIT, and NANOS3 compared to the ANAC group (P < 0.01). Furthermore, indirect immunofluorescence and flow cytometry demonstrated a significantly higher proportion of DDX4-positive cells in the acetate group (P < 0.01). These findings provide preliminary evidence that histone acetylation regulates chicken PGCs formation, offering a theoretical framework for the epigenetic induction of PGCs in vitro. This study enhances our understanding of the molecular mechanisms underlying PGCs development in poultry and contributes to advancements in avian reproductive technologies and genetic conservation.

01 Nov 2024·Theriogenology

Cryopreservation of the whole testes of Asian sea bass (Lates calcarifer) and its effects on apoptosis, germ cell-specific gene expression, germ cell transplantability, and DNA methylation

Article

Author: Pasomboon, P ; Sreebun, S ; Thongchaitriwat, S ; Booncherd, K ; Ichida, K ; Boonanuntanasarn, S ; Yazawa, R

Cryopreservation of spermatogonia could be a useful tool to preserve the genetic resources of fish, which could be further restored via germ cell transplantation. In this study, the protocol for the cryopreservation of the spermatogonia of Asian sea bass (Lates calcarifer), an economically important fishery resource in the Indo-West Pacific, was optimised. The impact of the cryopreservation technique on cell viability and apoptosis, expression of several genes related to immature germ cell markers, transplantability in allogeneic recipients, and global DNA methylation was evaluated. The slow-freezing method was performed for the cryopreservation of immature testis tissue, which contains a high proportion of spermatogonia. The optimal condition that yielded the highest recovery rate of post-thawed spermatogonia included a cryomedium containing Leibovitz's (L-15) medium and 10 % dimethyl sulfoxide, ice equilibration for 60 min before freezing, and subsequent thawing at 4 °C for 8 min. Moreover, a higher number of early and late apoptotic cells was detected in the cryopreserved than in the fresh testes, suggesting that apoptosis could result in reduced viability. The expression levels of dazl decreased in the cryopreserved testes; however, there were no significant differences in the expression levels of nanos2 or nanos3 between the fresh and cryopreserved testes. Although qRT-PCR showed lower vasa expression in cryopreserved testicular cells, in situ hybridisation showed expressed vasa in the cryopreserved testicular cells. Post-thawed spermatogonia could be incorporated into the genital ridge of allogeneic recipients, suggesting that cryopreserved spermatogonia exhibit transplantability characteristics. Compared with fresh testes, significant changes in the proportion of DNA methylation (decreased 5-mC and 5-caC) were observed in cryomedium-free testicular cells, whereas those of the cryopreserved cells were not significantly different. Therefore, the method we developed for the cryopreservation of the spermatogonia of Asian sea bass enabled post-thaw cells to retain several stemness characteristics and maintain their epigenetic stability.

01 Mar 2024·Reproductive Sciences

Divergent Roles of KLF4 During Primordial Germ Cell Fate Induction from Human Embryonic Stem Cells

Article

Author: Wang, Xiao ; Huang, Qi ; Lu, Shi ; Li, Zili ; Zhang, Xiaoke ; Fang, Fang

As a core transcriptional factor regulating pluripotency, Krüppel-like factor 4 (KLF4) has gained much attention in the field of stem cells during the past decades. However, few research have focused on the function of KLF4 during human primordial germ cell (PGC) specification. Here, we induced human PGC-like cells (hPGCLCs) from human embryonic stem cells (hESCs) and the derived hPGCLCs upregulated PGC-related genes, like SOX17, BLIMP1, TFAP2C, NANOS3, and the naïve pluripotency gene KLF4. The KLF4-knockout hESCs formed typical multicellular colonies with clear borders, expressed pluripotency genes, such as NANOG, OCT4, and SOX2, and exhibited no differences in proliferation capacity compared with wild type hESCs. Notably, KLF4 deletion in hESCs did not influence the induction of PGCLCs in vitro. In contrast, overexpression of KLF4 during PGC induction process inhibited the efficiency of PGCLC formation from hESCs in vitro. Overexpression of KLF4 may regenerate the naïve ground state in hESCs and results in repression for PGC specification. Thus, KLF4 could be a downstream target of human PGC program and the upregulation of KLF4 is prepared for late stage of germline development.

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NANOS3

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