GOLPH3

Last update 08 May 2025

Basic Info

Synonyms

Coat protein GPP34, golgi phosphoprotein 3, GOLPH3

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Introduction

Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.

100 Clinical Results associated with GOLPH3

100 Translational Medicine associated with GOLPH3

0 Patents (Medical) associated with GOLPH3

351

Literatures (Medical) associated with GOLPH3

01 Jun 2025·Biochemical Pharmacology

SUMOylation of RAD51 upregulates GOLPH3 expression and promotes cisplatin resistance in colon cancer cells by Sp1 transcriptional activity

Article

Author: Xie, Zhiyong ; Hong, Zhongshi ; Huang, Jingshan ; Qiu, Yi ; Qiu, Chengzhi ; Chen, Xiaojing ; Wu, Yuze ; Lin, Bingchen

Platinum-based chemotherapy is a first-line treatment for colon cancer. Previous studies have shown that Golgi phosphoprotein 3 (GOLPH3) overexpression drives platinum resistance in colon cancer and is associated with DNA damage repair (DDR). However, the mechanism by which DDR induces GOLPH3 expression remains unclear. This study investigates how RAD51 recombinase (RAD51) SUMOylation upregulates GOLPH3 expression and promotes platinum resistance in colon cancer. In DDP-resistant colon adenocarcinoma (COAD) cells, Specificity protein 1 (Sp1) and GOLPH3 were overexpressed, while N-myc downstream regulated 1 (NDRG1) was downregulated. Knockdown of Sp1 or GOLPH3 increased NDRG1 expression, inhibited COAD cell proliferation, promoted cell apoptosis, and enhanced cell sensitivity to cisplatin (DDP). Immunohistochemistry (IHC) and bioinformatics analyses of COAD tissues revealed a positive correlation between RAD51, SUMO1 and Sp1 expression. Sp1 was found to increase DDP resistance by transcriptionally activating GOLPH3 expression. RAD51 was SUMOylated by SUMO1 at the K57 site, and this modification decreased COAD cell sensitivity to DDP by enhancing Sp1 transcriptional activity. Furthermore, RAD51 overexpression led to upregulation of GOLPH3 and downregulation of NDRG1, promoting cell proliferation, inhibiting apoptosis, and increasing resistance to DDP. Conversely, the RAD51 mutant did not affect GOLPH3 expression or platinum resistance in vivo and in vitro. In conclusion, RAD51 SUMOylation at the K57 site enhances Sp1 transcriptional activity, thereby reducing colon cancer cell sensitivity to DDP by regulating GOLPH3 and NDRG1 expression. This discovery elucidates the molecular mechanism of DDR-induced GOLPH3 upregulation, offering a new perspective for overcoming DDP resistance in colon cancer.

10 Apr 2025·Nucleic Acids Research

Highly conserved ribosome biogenesis pathways between human and yeast revealed by the MDN1-NLE1 interaction and NLE1 containing pre-60S subunits

Article

Author: Wild, Klemens ; Cheng, Jingdong ; Sinning, Irmgard ; Hurt, Ed ; Thoms, Matthias ; Fiorentino, Federica ; Beckmann, Roland ; Denk, Timo ; Zeman, Jakub

AbstractThe assembly of ribosomal subunits, primarily occurring in the nucleolar and nuclear compartments, is a highly complex process crucial for cellular function. This study reveals the conservation of ribosome biogenesis between yeast and humans, illustrated by the structural similarities of ribosomal subunit intermediates. By using X-ray crystallography and cryo-EM, the interaction between the human AAA+ ATPase MDN1 and the 60S assembly factor NLE1 is compared with the yeast homologs Rea1 and Rsa4. The MDN1-MIDAS and NLE1-Ubl complex structure at 2.3 Å resolution mirrors the highly conserved interaction patterns observed in yeast. Moreover, human pre-60S intermediates bound to the dominant negative NLE1-E85A mutant revealed at 2.8 Å resolution an architecture that largely matched the equivalent yeast structures. Conformation of rRNA, assembly factors and their interaction networks are highly conserved. Additionally, novel human pre-60S intermediates with a non-rotated 5S RNP and processed ITS2/foot structure but incomplete intersubunit surface were identified to be similar to counterparts observed in yeast. These findings confirm that the MDN1-NLE1-driven transition phase of the 60S assembly is essentially identical, supporting the idea that ribosome biogenesis is a highly conserved process across eukaryotic cells, employing an evolutionary preservation of ribosomal assembly mechanisms.

18 Mar 2025·Proceedings of the National Academy of Sciences

Sequential evolution of resistance by western corn rootworm to multiple Bacillus thuringiensis traits in transgenic maize

Article

Author: Shrestha, Ram B. ; Kropf, Abigail L. ; St. Clair, Coy R. ; McCulloch, John B. ; Smith, Eliott M. ; Gassmann, Aaron J. ; Radosevich, Devin L. ; Brenizer, Ben D.

Transgenic crops that produce insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt) are grown worldwide to manage insect pests. Western corn rootworm is a serious pest of maize in the United States and is managed with Bt maize. In the United States, the commercial cultivation of a Bt crop requires an accompanying resistance-management strategy to delay the evolution of Bt resistance. One of the primary resistance-management strategies consists of non-Bt refuges along with a Bt crop that produces two Bt toxins (i.e., a pyramid) that kill the same pest species. This approach delays resistance because individuals with resistance to one toxin are killed by the second. However, if a pest species is resistant to one toxin in a pyramid, the effectiveness of a pyramid to delay resistance is compromised, potentially leading to the evolution of resistance to both toxins. Here, we apply a meta-analysis to demonstrate the sequential evolution of resistance by western corn rootworm to Bt maize producing Cry3Bb1 followed by resistance to Gpp34/Tpp35Ab1 maize, with resistance to each Bt toxin increasing in a linear manner over time. Additionally, we show that Bt-resistant western corn rootworm imposed substantial feeding injury, in the field, to maize containing a pyramid of Gpp34/Tpp35Ab1 and Cry3Bb1. To minimize the risk of sequential evolution of resistance to multiple transgenic traits, an emphasis should be placed on developing transgenic pyramids not compromised by prior resistance, and in cases where resistance is already present, larger non-Bt refuges and more diversified pest-management approaches should be applied.

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GOLPH3

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