CCDC61

Last update 23 Jan 2025

Basic Info

Synonyms

CCDC61, Centrosomal protein CCDC61, coiled-coil domain containing 61

+ [3]

Introduction

Microtubule-binding centrosomal protein required for centriole cohesion, independently of the centrosome-associated protein/CEP250 and rootletin/CROCC linker (PubMed:31789463). In interphase, required for anchoring microtubule at the mother centriole subdistal appendages and for centrosome positioning (PubMed:31789463). During mitosis, may be involved in spindle assembly and chromatin alignment by regulating the organization of spindle microtubules into a symmetrical structure (PubMed:30354798). Has been proposed to play a role in CEP170 recruitment to centrosomes (PubMed:30354798). However, this function could not be confirmed (PubMed:31789463). Plays a non-essential role in ciliogenesis (PubMed:31789463, PubMed:32375023).

100 Clinical Results associated with CCDC61

100 Translational Medicine associated with CCDC61

0 Patents (Medical) associated with CCDC61

Literatures (Medical) associated with CCDC61

01 Nov 2023·Developmental Cell

Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method

Article

Author: Roamio Franklin, Valar Nila ; van Breugel, Mark ; Kishore, Kamal ; Holder, James ; D'Santos, Clive ; Vitiello, Elisa ; Carden, Sarah ; Rosa E Silva, Ivan ; Ochi, Takashi ; Quarantotti, Valentina ; Gergely, Fanni

Centrosomes are the major microtubule-organizing centers in animals and play fundamental roles in many cellular processes. Understanding how their composition varies across diverse cell types and how it is altered in disease are major unresolved questions, yet currently available centrosome isolation protocols are cumbersome and time-consuming, and they lack scalability. Here, we report the development of centrosome affinity capture (CAPture)-mass spectrometry (MS), a powerful one-step purification method to obtain high-resolution centrosome proteomes from mammalian cells. Utilizing a synthetic peptide derived from CCDC61 protein, CAPture specifically isolates intact centrosomes. Importantly, as a bead-based affinity method, it enables rapid sample processing and multiplexing unlike conventional approaches. Our study demonstrates the power of CAPture-MS to elucidate cell-type-dependent heterogeneity in centrosome composition, dissect hierarchical interactions, and identify previously unknown centrosome components. Overall, CAPture-MS represents a transformative tool to unveil temporal, regulatory, cell-type- and tissue-specific changes in centrosome proteomes in health and disease.

15 Feb 2022·Journal of Cell ScienceQ2 · BIOLOGY

Lrrcc1 and Ccdc61 are conserved effectors of multiciliated cell function

Q2 · BIOLOGY

Article

Author: Rosnet, Olivier ; Viallat, Annie ; Nommick, Aude ; Schirmer, Claire ; Boutin, Camille ; Thomé, Virginie ; Loiseau, Etienne ; Bazellières, Elsa ; Kodjabachian, Laurent

ABSTRACTCiliated epithelia perform essential functions in animals across evolution, ranging from locomotion of marine organisms to mucociliary clearance of airways in mammals. These epithelia are composed of multiciliated cells (MCCs) harboring myriads of motile cilia, which rest on modified centrioles called basal bodies (BBs), and beat coordinately to generate directed fluid flows. Thus, BB biogenesis and organization is central to MCC function. In basal eukaryotes, the coiled-coil domain proteins Lrrcc1 and Ccdc61 have previously been shown to be required for proper BB construction and function. Here, we used the Xenopus embryonic ciliated epidermis to characterize Lrrcc1 and Ccdc61 in vertebrate MCCs. We found that they both encode BB components, localized proximally at the junction with striated rootlets. Knocking down either gene caused defects in BB docking, spacing and polarization. Moreover, their depletion impaired the apical cytoskeleton and altered ciliary beating. Consequently, cilia-powered fluid flow was greatly reduced in morphant tadpoles, which displayed enhanced mortality when exposed to pathogenic bacteria. This work illustrates how integration across organizational scales make elementary BB components essential for the emergence of the physiological function of ciliated epithelia.

01 Jun 2020·StructureQ2 · BIOLOGY

CCDC61/VFL3 Is a Paralog of SAS6 and Promotes Ciliary Functions

Q2 · BIOLOGY

Article

Author: Rosa E Silva, Ivan ; van Breugel, Mark ; Goldstein, Raymond E ; Blackford, Andrew N ; Barnabas, Deepak D ; Quarantotti, Valentina ; Jackson, Stephen P ; Jullien, Jerome ; Boselli, Francesco ; Blundell, Tom L ; Lin, Huawen ; Johnson, Christopher M ; McLaughlin, Stephen H ; Freund, Stefan M V ; Ochi, Takashi ; Kimata, Yuu ; Gergely, Fanni ; Dutcher, Susan K

Centrioles are cylindrical assemblies whose peripheral microtubule array displays a 9-fold rotational symmetry that is established by the scaffolding protein SAS6. Centriole symmetry can be broken by centriole-associated structures, such as the striated fibers in Chlamydomonas that are important for ciliary function. The conserved protein CCDC61/VFL3 is involved in this process, but its exact role is unclear. Here, we show that CCDC61 is a paralog of SAS6. Crystal structures of CCDC61 demonstrate that it contains two homodimerization interfaces that are similar to those found in SAS6, but result in the formation of linear filaments rather than rings. Furthermore, we show that CCDC61 binds microtubules and that residues involved in CCDC61 microtubule binding are important for ciliary function in Chlamydomonas. Together, our findings suggest that CCDC61 and SAS6 functionally diverged from a common ancestor while retaining the ability to scaffold the assembly of basal body-associated structures or centrioles, respectively.

Analysis

Perform a panoramic analysis of this field.

view full example data

Chat with Hiro

Get started for free today!

Accelerate Strategic R&D decision making with Synapse, PatSnap’s AI-powered Connected Innovation Intelligence Platform Built for Life Sciences Professionals.

Start your data trial now!

Synapse data is also accessible to external entities via APIs or data packages. Empower better decisions with the latest in pharmaceutical intelligence.

Bio

Bio Sequences Search & Analysis

Chemical

Chemical Structures Search & Analysis

CCDC61

Basic Info

Related

Analysis