VASP

Last update 08 May 2025

Basic Info

Synonyms

vasodilator stimulated phosphoprotein, Vasodilator-stimulated phosphoprotein, VASP

Introduction

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

100 Clinical Results associated with VASP

100 Translational Medicine associated with VASP

0 Patents (Medical) associated with VASP

1,452

Literatures (Medical) associated with VASP

01 Jun 2025·Molecular and Cellular Probes

Silymarin plus doxorubicin exerts the anti-hepatocellular carcinoma effects via Wnt, apoptosis, autophagy and angiogenesis pathways

Article

Author: Gao, Zehai ; Yuan, Baohong ; Wang, Ruotian ; Guo, Feng ; Xiang, An-Dong ; Mirzeei, Hameed

BACKGROUNDThe biggest cause of death worldwide is liver cancer. Despite several initiatives and successes in treatment techniques, only a little improvement has been attained. In order to control this cancer, new therapeutic strategies are therefore required. Here, we evaluated the effects of doxorubicin and the milk thistle plant phytochemical Silymarin on liver cancer through apoptosis, autophagy, and Wnt signaling.METHODSSilymarin both alone and together with doxorubicin was administered to induce cytotoxicity in the H22 cell line. qRT-PCR and Western blot analyses, the genes related to autophagy, Wnt signals, and cell death were examined.RESULTSDoxorubicin and Silymarin both individually and combined dramatically slowed down H22 cells growth. Additionally, there was a significant drop in the Bcl-2 protein and a considerable rise in the caspase 8 and Bax proteins. LC3-I, LC3-II, and Beclin 1 have been all shown to be significantly elevated. Moreover, there was a substantial decrease in the expression of genes involved in the Wnt pathway, including cyclin D1, β-catenin, ZEB1, and Twist. The levels of AMPK were decreased in Silymarin with Doxorubicin alone and in combination, whereas VASP, VEGF, and HIF-1a were lowest.CONCLUSIONSilymarin may enhance anti-tumor effects of doxorubicin through modulating autophagy, angiogenesis, and apoptosis, in-vitro.

01 May 2025·Arteriosclerosis, Thrombosis, and Vascular Biology

Prasugrel Intermediate Metabolite Modulates Platelet Inhibition by Negatively Interfering With an Active Metabolite: An Ex Vivo, In Vitro, and In Silico Study

Article

Author: Taus, Francesco ; Gottardo, Rossella ; Meneguzzi, Alessandra ; Bortolotti, Federica ; Cattaneo, Marco ; Valgimigli, Marco ; Tagliaro, Franco ; Castelli, Marco ; Gargiulo, Giuseppe ; Fava, Cristiano ; Minuz, Pietro ; Ribeiro, Rui P. ; Landi, Antonio ; Gragnano, Felice ; Verlato, Giuseppe ; Baldessari, Filippo ; Giorgetti, Alejandro

BACKGROUND:Prasugrel is converted into prasugrel active metabolite (PAM; R-138727) through the cytochrome P450–mediated conversion of an intermediate metabolite (PIM; R-95913). It is unknown whether PIM exerts any biological function. The FABOLUS-FASTER trial (Facilitation Through Aggrastat or Cangrelor Bolus and Infusion Over Prasugrel: A Multicenter Randomized Open-Label Trial in Patients With ST-Elevation Myocardial Infarction Referred for Primary Percutaneous Intervention) showed that chewed prasugrel does not improve bioactivity, in spite of accelerated PAM kinetics.METHODS:PIM and PAM pharmacokinetics were assessed by mass spectrometry in blood samples collected from ST-segment–elevation myocardial infarction patients randomized to chewed (n=17) or integral (n=15) 60 mg prasugrel. The ex vivo and in vitro effects of PAM and PIM were assessed on ADP-induced platelet activation. The binding sites of PIM and PAM were investigated by molecular dynamics simulation.RESULTS: Chewed prasugrel was associated with higher PIM levels compared with integral prasugrel: PIM median area under the curve (25–75 p): 73 (41.5–92.0) versus 33 (0.0–50.0) ng·h/mL ( P <0.05). PIM plasma concentrations negatively correlated with inhibition of ADP-induced platelet aggregation, which strongly correlated to the PAM/PIM ratio (ρ=0.782; P <0.001; n=30) than PAM, suggesting an antagonistic role of PIM on PAM-induced P2Y 12 inhibition. Subsequent in vitro tests confirmed the dose-dependent, reversible antagonistic effect of PIM on PAM inhibition of aggregation (maximum effect, −49.5% [95% CI, −54.4% to −44.6%]; P <0.001), confirmed by P-selectin expression and vasodilator-stimulated phosphoprotein phosphorylation as readouts at the signaling level. At molecular dynamics simulations of the drug-receptor systems, PIM accommodates through noncovalent reversible binding in the same PAM-binding site, distinct from that of 2-methylthio-adenosine-5′-diphosphate. CONCLUSIONS: PIM negatively interferes with PAM, thereby reducing its inhibitory activity, likely competing at the P2Y 12 receptor–binding site. REGISTRATION: URL: https://www.clinicaltrials.gov ; Unique identifier: NCT02978040. URL: https://www.clinicaltrialsregister.eu ; Unique identifier: EudraCT 2017-001065-24.

01 May 2025·International Journal of Biological Macromolecules

Comparative transcriptomic and co-expression network analysis identifies key gene modules involved in heat stress responses in goats

Article

Author: Rout, Pramod Kumar ; Dige, Mahesh Shivanand ; Kataria, Ranjit Singh ; Kaushik, Rakesh ; Gurao, Ankita ; Kumar, Amit ; Singh, Manoj Kumar ; Mehrotra, Arnav

Heat stress significantly affects livestock production, particularly in tropical regions where temperatures often exceed animals' comfort zones. This study investigates the molecular mechanisms of heat stress tolerance in Jamunapari goats (Capra hircus) through transcriptomic analysis, gene co-expression network construction, and hub gene identification. Female goats (1-2 years old) were monitored during high Thermal Humidity Index (THI) in June and normal THI in March. Based on heat tolerance and physiological parameters, goats were classified into Thermo-Neutral (TNG) and Heat-Stress (EHSG) groups. Differential gene expression analysis revealed 133 upregulated genes and 501 downregulated genes in the EHSG group. Upregulated pathways included NF-kappa B signaling, MAPK signaling, and cytokine-cytokine receptor interactions, while downregulated genes were linked to IL-17 signaling and platelet activation. Notably, the small heat shock proteins (CRYAB) and aquaporins (AQP11) were significantly downregulated. Weighted Gene Co-expression Network Analysis (WGCNA) identified key gene modules associated with Iberia Heat Tolerance Coefficient and respiration rate. Hub genes such as TUFM, TOMM40, BCSL1, VCL, VASP, ITGB, and VWF were critical for adaptation to heat stress. These findings enhance our understanding of heat stress resilience, offering potential targets for breeding programs aimed at improving livestock tolerance to heat stress in tropical environments.

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