DDX49

Last update 08 May 2025

Basic Info

Synonyms

Dbp8, DDX49, DEAD box protein 49

+ [3]

Introduction

ATP-dependent RNA helicase that plays a role in various aspects of RNA metabolism including the regulation of mRNA export and the levels of pre-ribosomal RNA. Regulates the stability and synthesis of pre-ribosomal RNA and thereby regulates cell proliferation (PubMed:29618122). Possesses also antiviral activity by recognizing gammaherpesvirus transcripts in the context of lytic reactivation (PubMed:36298642).

100 Clinical Results associated with DDX49

100 Translational Medicine associated with DDX49

0 Patents (Medical) associated with DDX49

Literatures (Medical) associated with DDX49

02 Jan 2025·Growth Factors

Expression of DDX49 in breast cancer and its mechanism regulating the proliferation and metastasis of breast cancer cells

Article

Author: Wang, Yuanbin ; Yang, Lijun ; Ma, Ruimin ; Yang, Qing ; Li, Xiangli ; Wu, Zhihao

DEAD-box RNA helicase (DDX) is linked to the invasion, drug resistance, proliferation, and epithelial-mesenchymal transition of tumour cells. This study examined the potential mechanisms of DDX49 in breast cancer. The expression of DDX49 in breast cancer tissues and cells was evaluated. The effects of DDX49 on proliferation, invasion, migration and apoptosis of breast cancer cells were evaluated. The expression of proteins associated with the JAK/STAT pathway was examined. A xenograft tumour model was established. DDX49 expression is elevated in breast cancer tissues and cell lines. shDDX49 suppressed the ability of breast cancer cells to proliferate, invade, and migrate, but promoted apoptosis. Conversely, overexpression of DDX49 exerted an opposite effect. The activation of the JAK-STAT signalling pathway is inhibited by the shDDX49. shDDX49 efficiently inhibits tumour growth in mice with breast cancer. shDDX49 may hinder the growth and spread of breast cancer cells by inhibiting the JAK-STAT pathway.

01 Dec 2024·Royal Society Open Science

Identification of a novel nuclease activity in human DDX49 helicase

Article

Author: Kapllanaj, Fiorela ; Martin, Louise ; Bolt, Edward L. ; Anandavijayan, Sabesan ; Downs, Olivia ; Lou-Hing, Anna ; Killelea, Tom ; Parkes, Ashley J.

Human DDX49 is an emerging target in cancer progression and retroviral diseases through its essential roles in nucleolar RNA processing. Here, we identify nuclease activity of human DDX49, which requires active site aspartate residues within a conserved region of metazoan DDX49s that is absent from yeast and archaeal DDX49 homologues. We provide evidence that DDX49 nuclease activity is facilitated by its helicase activity. Using CRISPR-Cas9 genetic editing, we show that a heterozygous ( DDX49+/− ) U2OS cell line is defective at cell migration, a phenotype supporting the association of DDX49 with cancer cell invasiveness. Measurement of RNAs in DDX49+/− indicates that DDX49 is required to sustain levels of 5.8S rRNA.

01 Sep 2024·Genomics

Transcriptomic analysis of different intramuscular fat contents on the flavor of the longissimus dorsi tissues from Guangling donkey

Article

Author: Wang, Xi ; Li, Wufeng ; LiLi

BACKGROUNDIn this study, researchers aimed to explore the impact of intramuscular fat (IMF) concentration on the flavor of donkey meat, specifically in the longissimus dorsi muscle of Guangling donkeys. The internal volatile organic compounds that cause the flavor differences between donkey muscles are not clear at present. Transcriptomic technologies were utilized to analyze gene expression and its relationship to donkey meat flavor.METHODThirty Guangling donkeys had their IMF content evaluated in the longissimus dorsi muscle. Based on IMF content, 16 donkeys of similar ages were divided into two groups: low-fat (L) and high-fat (H). Headspace solid-phase microextraction Gas chromatography-mass spectrometry (HS-SPME-GC-MS) and headspace solid phase microextraction mass spectrometry were used to identify potential flavor components that differed between the two groups.RESULTSFive key volatile substances were identified, and WGCNA and KEGG analysis was conducted to analyze the genes associated with these substances. The results showed that pathways like PPAR signaling, nucleotide excision repair, glucagon signaling, arachidonic acid metabolism, and glycolysis/glycogenesis were involved in lipid deposition. Additionally, a gene-gene interaction network map was constructed, highlighting the importance of hub genes such as EEF2, DDX49, GAP43, SNAP25, NDUFS8, MRPS11, RNASEH2A, POLR2E, POLR2C and ALB in regulating key flavor substances.CONCLUSIONThis study provided valuable insights into the regulation of genes and protein expression related to flavor substances in donkey meat. It also deepened understanding of the influence of IMF on flavor and laid a foundation for future molecular breeding improvements in Guangling donkeys.

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DDX49

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