CMAHP

Last update 08 May 2025

Basic Info

Synonyms

CMAH, CMAHP, CMP-NeuAc hydroxylase-like protein

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Introduction

Sialic acids are components of carbohydrate chains of glycoconjugates and are involved in cell-cell recognition and cell-pathogen interactions. That protein has no CMP-N-acetylneuraminate monooxygenase activity and is not able to convert CMP-N-acetylneuraminic acid (CMP-Neu5Ac) into its hydroxylated derivative CMP-N-glycolylneuraminic acid (CMP-Neu5Gc), a sialic acid abundantly expressed at the surface of many cells in vertebrates (PubMed:9624188). However, it may play a role in Wnt signaling (PubMed:19890979).

100 Clinical Results associated with CMAHP

100 Translational Medicine associated with CMAHP

0 Patents (Medical) associated with CMAHP

219

Literatures (Medical) associated with CMAHP

01 Apr 2025·Cancer Letters

Dietary intake of the red meat-derived glycan Neu5Gc fuels colorectal cancer through up-regulation of Wnt signaling pathway

Article

Author: Paulino, Amanda Carlos ; Lemos, Felipe ; Willert, Karl ; Varki, Nissi ; Kawanishi, Kunio ; De Oliveira, Rodrigo P ; Todeschini, Adriane Regina ; Lopes, Ana Luiza ; Caloba, Philippe ; Varki, Ajit ; Morgado-Diaz, Jose Andres ; Thaumaturgo, Mariana A S ; Machado, João C ; Alisson-Silva, Frederico ; Araújo, Wallace Martins ; Läubli, Heinz

Colorectal cancer (CRC) is a significant health concern, often linked to western diets, particularly red meat consumption. Several mechanisms, such as the high heme iron content, the formation of N-nitroso compounds (NOCs), heterocyclic amines (HCAs), and polycyclic aromatic hydrocarbons (PAHs), have been suggested to explain red meat's cancer-promoting effects. However, these factors are also found in fish and poultry, which are not linked to CRC risk. A new hypothesis attributes red meat's impact on CRC to its high content of a nonhuman glycan. While most mammals express N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), humans express only Neu5Ac due to the loss of the CMAH enzyme. A red meat-rich diet leads to the incorporation of Neu5Gc into human cells, triggering an antibody-mediated inflammatory process known as xenosialitis. This study shows that Neu5Gc incorporation into CRC cells activates the Wnt/β-catenin signaling pathway, promoting cell proliferation. In a murine model lacking CMAH, a Neu5Gc-enriched diet induced intestinal polyp growth, with more malignant characteristics. Additionally, Neu5Gc incorporation in intestinal cells increased the expression of genes downstream of Wnt signaling. These findings reveal, for the first time in an in vivo model, a mechanism independent of immune response, where red meat consumption accelerates tumor progression through Neu5Gc incorporation. This activation of the Wnt/β-catenin signaling pathway provides new insight into how red meat consumption may influence CRC progression.

01 Mar 2025·International Journal of Biological Macromolecules

Effect of CHO cell line constructed with CMAH gene-directed integration on the recombinant protein expression

Article

Author: Yuan, Jing-Jia ; Bai, Zhi-Yuan ; Geng, Shao-Lei ; Zhang, Min ; Wang, Tian-Yun ; Yun, Yi-Fei ; Cao, Xiang-Xiang ; Sun, Qiu-Li ; Mi, Chun-Liu ; Wang, Xiao-Yin

Chinese hamster ovary (CHO) cells are the most widely used platform for recombinant therapeutic protein (RTP) production. Traditionally, the development of CHO cell lines has mainly depended on random integration of transgenes into the genome, which is not conducive to stable long-term expression. Cytidine monophosphate N-acetylneuraminic acid hydroxylase (CMAH) is expressed in CHO cells and produces N-hydroxyacetylneuraminic acid, which may cause a human immune response. However, the effects of transgene integration at the CMAH site on RTP expression in CHO cells remain unclear. In this study, we selected CMAH gene, which is lacking in humans, as the target site to construct recombinant CHO cell line using the CRISPR/Cas9 technique. Erythropoietin (EPO) and EGFP integration at the CMAH site resulted in more stable expression levels and lower heterogeneity than random integration. In addition, the proportion of N-glycosylation levels in the EPO glycoside of CMAH integration site also changed. In conclusion, CMAH site integration improved the stability of RTP expression in CHO cells.

01 Feb 2025·Reproduction in Domestic Animals

Effects of Electroporation Timing and Cumulus Cell Attachment on In Vitro Development and Genome Editing of Porcine Embryos

Article

Author: Nagahara, Megumi ; Otoi, Takeshige ; Lin, Qingyi ; Tanihara, Fuminori ; Torigoe, Nanaka ; Nakai, Aya ; Hirata, Maki ; Nakayama, Yuichiro ; Liu, Bin

ABSTRACTPig genome editing using the oviductal nucleic acid delivery (GONAD) method with electroporation would allow the efficient obtention of genetically modified pigs. However, oocytes and zygotes at various stages after ovulation must be targeted, and cumulus cell attachment and mosaic mutations are major obstacles. Therefore, we investigated whether two parameters (electroporation timing and the cumulus cell attachment) influence the effectiveness of multiplex genome editing by electroporation in porcine oocytes or zygotes. Three gRNAs targeting either GGTA1, CMAH or B4GALNT2 were introduced individually into oocytes and zygotes with and without cumulus cells at three different time points, 0 h before in vitro fertilisation (IVF) and 5 h and 10 h after IVF initiation. The introduction of gRNAs into oocytes and zygotes did not significantly affect the rates of blastocyst formation and total mutation of the resulting blastocysts irrespective of cumulus cell attachment and electroporation timing. In conclusion, the electroporation timing and the cumulus cell attachment did not interfere with the efficient delivery of the CRISPR/Cas9 system to the oocytes/zygotes, indicating that porcine genome editing in the oviduct using GONAD method may be possible.

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CMAHP

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