What is the mechanism of Beriplast-P?

Beriplast-P is a fibrin sealant used in surgical procedures to control bleeding and to promote tissue sealing and wound healing. Its mechanism of action is based on the natural coagulation process but is amplified through a biocompatible formulation to achieve rapid and effective hemostasis.

Beriplast-P is composed of several key components: human fibrinogen, human thrombin, aprotinin, and calcium chloride. Each of these components has a specific role in the coagulation cascade, working synergistically to achieve hemostasis.

The process begins with the preparation of Beriplast-P, which involves mixing its components to create two separate solutions. The first solution contains fibrinogen and aprotinin, while the second solution includes thrombin and calcium chloride. These solutions are stored separately until the time of application to prevent premature clotting.

During surgical application, the two solutions are mixed at the site of bleeding using a dual-chambered applicator. When the fibrinogen and thrombin come into contact with each other, a rapid series of biochemical reactions is initiated. Thrombin converts fibrinogen into fibrin monomers, which then spontaneously polymerize to form a stable fibrin clot. The calcium chloride in the second solution acts as a cofactor that enhances the activity of thrombin and stabilizes the fibrin clot.

Aprotinin, a protease inhibitor, plays a crucial role by preventing premature breakdown of the fibrin clot. It inhibits fibrinolysis, the process by which the body dissolves blood clots, thereby ensuring that the fibrin matrix remains intact long enough to allow for effective tissue sealing and hemostasis.

The fibrin clot formed by Beriplast-P adheres to the tissue surfaces, providing a mechanical barrier that seals the wound and minimizes blood loss. This clot not only achieves immediate hemostasis but also supports the body's natural wound healing processes by providing a scaffold for cell migration and tissue regeneration.

One of the advantages of Beriplast-P is its biocompatibility. Being derived from human plasma proteins, it is well tolerated by the body and integrates seamlessly with the patient’s own tissue. This minimizes the risk of adverse reactions and promotes faster and more efficient healing.

In summary, the mechanism of Beriplast-P involves a carefully orchestrated cascade of biochemical reactions that mimic the body's natural clotting process but in a more controlled and amplified manner. The combination of fibrinogen, thrombin, aprotinin, and calcium chloride creates a stable fibrin clot that not only stops bleeding but also aids in tissue sealing and wound healing. This makes Beriplast-P an invaluable tool in various surgical procedures where rapid and effective hemostasis is crucial.