Delving into the Latest Updates on Dachaihu Decoction with Synapse

Overview

Basic Info

Drug Type

Herbal medicine

Synonyms

JUNKOU Daisaikoto FC Extract Fine Granules for Ethical Use, OHSUGI Daisaikoto Extract Granules G, OHSUGI Daisaikoto Extract T Tablets

+ [17]

Target-

Mechanism-

Therapeutic Areas

Immune System DiseasesNervous System DiseasesCardiovascular Diseases+ [4]

Active Indication

HeadacheObesityArteriosclerosis+ [14]

Inactive Indication-

Originator Organization

Sanwa Shoyaku Co., Ltd.

Active Organization

Sanwa Shoyaku Co., Ltd.Ohsugi Pharmaceutical Co., Ltd.

Inactive Organization-

Drug Highest PhaseApproved

First Approval Date

JP (18 Jul 1986),

ArteriosclerosisAsthmaCholecystitis+ [12]

Regulation-

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Background:The rise of antibiotic-resistant bacteria necessitates the discovery of new, safe, and bioactive antimicrobial compounds. The antibacterial and antibiofilm activity of microalgae makes them a potential candidate for developing natural antibiotics to limit microbial infection in various fields.Objective:This study aimed to analyze the antibacterial effect of the methanolic extract of Schizochytrium sp. S31 microalgae by broth microdilution and spot plate assays.Methods:The antibacterial effects of Schizochytrium sp. S31 extract was studied on gramnegative pathogens, Pseudomonas aeruginosa, Escherichia coli 35218, Klebsiella pneumonia, which cause many different human infections, and the gram-positive pathogen Streptococcus mutans. At the same time, the antibiofilm activity of the Schizochytrium sp. S31 extract on Pseudomonas aeruginosa and Escherichia coli 35218 bacteria were investigated by crystal violet staining method.Results:Schizochytrium sp. S31 extract at a 60% concentration for 8 hours displayed the highest antibacterial activity against P. aeruginosa, E. coli 35218, and K. pneumonia, with a decrease of 87%, 92%, and 98% in cell viability, respectively. The experiment with Streptococcus mutans revealed a remarkable antibacterial effect at a 60% extract concentration for 24 hours, leading to a notable 93% reduction in cell viability. Furthermore, the extract exhibited a dose-dependent inhibition of biofilm formation in P. aeruginosa and E. coli 35218. The concentration of 60% extract was identified as the most effective dosage in terms of inhibition.Conclusion:This research emphasizes the potential of Schizochytrium sp. S31 as a natural antibacterial and antibiofilm agent with promising applications in the pharmaceutical sectors. This is the first study to examine the antibacterial activity of Schizochytrium sp. S31 microalgae using broth microdilution, spot plate assays, and the antibiofilm activity by a crystal staining method. The findings of this study show that Schizochytrium sp. S31 has antibacterial and antibiofilm activities against critical bacterial pathogens.

01 Dec 2023·Current Microbiology

Induced Resistance Mechanism of Bacillus velezensis S3-1 Against Pepper Wilt

Article

Author: Jiang, Qiuyan ; Xiao, Ming ; Yu, Yating ; He, Xingjie ; Yang, Ning ; Dai, Jiawei ; Xu, Zhaofeng ; Tang, Xiaorong ; Fan, Yongjie

In recent years, pepper wilt has emerged as a pivotal constraint on pepper yield augmentation. Bacillus velezensis S3-1, with a wide array of hosts, can be used as both a biocontrol agent and biofertilizer. Nonetheless, the precise mechanisms underpinning its employment in combating pepper wilt remain cloaked in ambiguity. In our study, we found that B. velezensis S3-1 could significantly inhibit Fusarium sp. F1^T that caused pepper wilt. S3-1 could effectively inhibit both the growth and germination of F1^T conidia, leading to a reduction in the spore germination percentage from 83.2 to 37.1% in vitro experiments. Additionally, leaf detachment experiments revealed that the volatile compounds produced by S3-1 could inhibit the spread of pepper leaf spot area. Moreover, we observed a significant decrease in the content of malondialdehyde (MDA) in pepper treated with S3-1, along with a significant increase in the content of soluble protein, polyphenol oxidase (PPO), peroxidase (POD), and phenylalanine ammonia-lyase (PAL) in pepper. Furthermore, RT-PCR analysis showed that the expression of the defense genes CaPR 1 and CaPIN II in pepper after treatment with S3-1 was significantly upregulated, suggesting that S3-1 had the potential to induce systemic resistance in pepper, thereby enhancing its disease resistance. Hence, our findings suggest that S3-1 can be a promising biocontrol agent for managing pepper wilt in modern agriculture.

01 Nov 2023·Combinatorial Chemistry & High Throughput Screening

Study on the Mechanism of Dachaihu Decoction in the Treatment of Acute Pancreatitis Based on Artificial Intelligence Combined with in vivo Experiments

Article

Author: Zhang, Yan ; Wang, Pei ; Zhang, Xiujiang ; Li, Wei ; Pang, Yuming ; Li, Sujie ; Meng, Zhe

Background and Aim:To explore the possible mechanism of Dachaihu Decoction (DCHD) in the treatment of AP, and use in vivo experiments to verify.Methods:The targets and active ingredients of DCHD in the treatment of AP were obtained through network pharmacology, and the preliminary verification was carried out by molecular docking. Caerulein was used to develop the AP rat model. H&E staining was performed to observe variations in pancreatic tissue. Western blot and RT-qPCR were conducted to evaluate the associated proteins and mRNA.Results:The network pharmacology and molecular docking results showed that the key targets (EGFR, TNF, SRC, VEGFA and CTNNB1) and key active components (beta-sitosterol, stigmasterol, baicalein, quercetin, and kaempferol) of DCHD in the treatment of AP had good binding. H&E staining revealed that rat pancreatic tissues considerably damaged post caerulein intervention, and it has also been suggested that DCHD ameliorates damage to pancreatic tissue. Simultaneously, EGFR, TNF, SRC, VEGFA protein, and mRNA expression levels were increased in the model group compared to the blank group (P < 0.01), whereas CTNNB1 expression was found to be decreased in the model group (P < 0.01). Compared with the model group, the protein expression levels of EGFR, TNF, SRC, and VEGFA in the treatment group were down-regulated (P < 0.01), and CTNNB1 was up-regulated (P < 0.05).Conclusion:DCHD protects pancreatic tissues and improves symptoms in AP rats by upregulating CTNNB1 protein and mRNA while inhibiting EGFR, TNF, SRC, and VEGFA protein and mRNA expression.

100 Deals associated with Dachaihu Decoction

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R&D Status

10 top approved records.

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Indication	Country/Location	Organization	Date
Headache	JP	Ohsugi Pharmaceutical Co., Ltd.	02 Sep 1986
Obesity	JP	Ohsugi Pharmaceutical Co., Ltd.	02 Sep 1986
Arteriosclerosis	JP	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Asthma	JP	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Cholecystitis	JP	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Cholelithiasis	JP	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Constipation	JP	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Gastritis	JP	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Hemiplegia	JP	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Hemorrhage	JP	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Hemorrhoids	JP	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Hypertension	JP	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Jaundice	JP	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Neuralgia	JP	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Neurasthenia	JP	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Obesity, Morbid	JP	Sanwa Shoyaku Co., Ltd.	18 Jul 1986
Sleep Initiation and Maintenance Disorders	JP	Sanwa Shoyaku Co., Ltd.	18 Jul 1986