Nucleic acid aptamers, known as chemical antibodies, demonstrate remarkable affinity and specificity for targets. Therefore, aptamers are proposed as an alternative to an antibody in extensive applications. However, nucleic acid aptamers exhibit poor tolerance to degradation by nucleases, which severely hampers their biological applications. Herein, we developed a biological regulation pattern for aptamers by utilizing small-molecule-mediated terminal manipulation, which could prevent the interaction of DNA aptamers with exonucleases and help aptamers persist in the desired conformation with high stability. Diagonal T-T bases were designed in the ends of aptamers and could be chemically cross-linked with trioxsalen via photocatalyzed cycloaddition. Aptamers with different patterns of terminal T-T cross-linking sites were synthesized. Experimental investigation and molecular dynamics simulations combinedly revealed that the cross-linking efficiency of ends depended on multiple factors: (i) the number of T-T cross-linking sites in the terminal sequences, (ii) the spatial conformation of aptamers, and (iii) the competitive binding ability of the T-T sites with trioxsalen compared to other base sites. The aptamers with locked ends exhibited superior exonuclease resistance, especially with both 3'- and 5'-cross-linked ends, thus demonstrating a great target binding capability. Notably, in the application exploration, the terminal locked aptamers, which bound to receptor-binding domains on SARS-CoV-2, showed superior performance in virus infection inhibition. This work puts forward a paradigm to develop a biological regulation pattern for aptamers based on chemical terminal manipulation of DNA, potentially promoting the clinical applications of nucleic acid drugs.

01 Mar 2025·Journal of Basic Microbiology

Harnessing Biocontrol Potential of Streptomyces rochei Against Pythium aphanidermatum: Efficacy and Mechanisms

Article

Author: Nakkeeran, Sevugapperumal ; Paramasivan, Mookkan ; Anandham, Rangasamy ; Karthikeyan, Muthusamy ; Johnson, Iruthayasamy ; Kavitha, Rangasamy ; Priya, Dhanabalan Shanmuga

ABSTRACTTomato (Solanum lycopersicum) and chilli (Capsicum annuum) are globally significant vegetable crops susceptible to damping‐off disease caused by Pythium aphanidermatum, leading to substantial yield losses. The study aimed to document the biocontrol and plant growth promotion potential of Streptomyces rochei against damping‐off disease in tomato and chilli. The actinobacterial isolates ACS18 followed by ACT30, and AOE12 were accomplished as the most effective antagonists against P. aphanidermatum in vitro. Molecular characterization confirmed these isolates as members of Streptomyces genus, with ASH 18 the top performer identified as S. rochei isolate. Analysis of biomolecule through GC‐MS during ditrophic interaction between pathogen and S. rochei showed the presence of various antifungal metabolites which were directly related to suppression of the pathogen. Subsequently, S. rochei was formulated into a talc‐based preparation and used as seed treatment and soil application against damping‐off. In greenhouse trials, significant reductions in damping‐off incidence were observed, Furthermore, seedlings treated with S. rochei displayed enhanced root and shoot lengths compared to the uninoculated controls. These benefits potentiate S. rochei as a promising biocontrol agent and demonstrating its dual benefits of disease suppression and promotion of seedling growth.

01 Feb 2025·Journal of the Iranian Chemical Society

Europium activated bismuth containing double perovskite catalyzed synthesis of coumarin derivatives and its cytotoxicity evaluation

Author: Das, Bimal ; Bonnada, Nagamani Naidu ; Datta, Deepshikha ; Tejeswararao, Dharmana

Abstract: Using NaBaBi_1-xEu_xMoO₆:Eu³⁺(x = 0.0-0.24) europium activated bismuth containing double perovskite materials, a novel heterogeneous catalytic procedure was developed to prepare coumarin and its modified coumarins.Double perovskite catalyst predicts great activity toward a wide range of phenols with variety of that have been substituted with ketoesters in precise yields over short response times during the production of coumarin.Standard protocols were used to prepare various coumarin derivatives, characterized by spectral techniques and eventually evaluated against COLO205, A549, THP-1, B16 and U937 human cancer cells.Compared to the standard drug Etoposide, hybrid 3f demonstrated a higher affinity and better binding interactions with the above cancer cells.UV-fluorescence spectra were recorded for selected coumarin derivativesHowever, it is stated here that these mol. hybrids of coumarin could serve as a great starting point for future research to create effective anticancer agents.

100 Deals associated with Trioxsalen

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KEGG	Wiki	ATC	Drug Bank
D01034	Trioxsalen	D05BA01 D05AD01	DB04571

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