Urolithin A

DS-01® is the first broad-spectrum synbiotic clinically shown to increase production of both Urolithin A and butyrate—two key microbial metabolites linked to whole-body health Presented at Digestive Disease Week® (DDW) 2025, the findings expand DS-01®'s clinical foundation and reinforce the potential of microbiome-targeted products to impact multiple biological systems BOSTON, May 5, 2025 /PRNewswire/ -- Microbiome science company Seed Health presented clinical data on its flagship innovation, DS-01® Daily Synbiotic, at Digestive Disease Week® (DDW) 2025. Findings from a randomized, placebo-controlled clinical trial in healthy adults demonstrated that DS-01® increased production of two key microbial metabolites—Urolithin A and butyrate—implicated in gut barrier function, metabolism, and healthy aging. The trial also found reductions in C-Reactive Protein (CRP), a widely used marker of systemic inflammation, suggesting DS-01®'s potential to influence immune pathways. The findings underscore DS-01®'s impact beyond microbial composition, demonstrating measurable biological function through the production of microbiome-mediated metabolites. The company presented two posters during the "Prebiotics, Probiotics and Synbiotics in Health and Disease" session. Presentation Highlights Effects of a novel, broad-spectrum synbiotic on Urolithin A production: A randomized, placebo-controlled clinical trial This randomized, placebo-controlled clinical trial evaluated DS-01® for its ability to increase production of Urolithin A—a microbiome-derived metabolite linked to mitochondrial function, cellular energy, metabolic health, intestinal barrier integrity, and healthy aging. DS-01® provides the natural machinery to produce Urolithin A, delivering both a polyphenol precursor in its prebiotic outer capsule and key microbes in its probiotic inner capsule. Overall, this is the first study to demonstrate that a broad-spectrum synbiotic can increase Urolithin A production in humans. Key findings include: Rapid and Sustained Urolithin A Increase: DS-01® increased urinary Urolithin A levels by approximately 100-fold by Day 7 (P<0.0001), with levels sustained through Day 91. 100% Conversion to Urolithin A Producers: By Day 91, 100% of participants in the DS-01® arm became Urolithin A producers, compared to 44% in the placebo arm (P<0.01). Increased Lactobacillus spp. Abundance: DS-01® significantly increased the abundance of specific Lactobacillus species known to convert dietary polyphenols into Urolithin A precursors through Day 91 (P<0.001). A novel, broad-spectrum synbiotic increases butyrate production and decreases CRP: A randomized, placebo-controlled clinical trial This randomized, placebo-controlled trial evaluated DS-01® for its impact on butyrate and CRP. Butyrate is a key short-chain fatty acid (SCFA) produced by the gut microbiome, which fuels the cells that line the colon and supports immune function, gut barrier integrity, and metabolic signaling. CRP is a systemic marker of inflammation. The study is among the first to show that a broad-spectrum synbiotic can significantly increase butyrate levels in humans. Key findings include: Increased Butyrate Production: The synbiotic significantly increased fecal butyrate levels through Day 91 (P<0.03) in participants with low baseline butyrate levels; no change was observed in the placebo arm. Reduction in a Key Systemic Inflammatory Marker: There was a reduction in CRP, a marker of systemic inflammation, associated with the colonization of DS-01® species through Day 91 (P<0.02). Enhanced Microbiome Diversity: Participants in the synbiotic arm showed significantly higher diversity of Bifidobacterium and Lactobacillus species at all time points compared to placebo through Day 91 (P<0.0001). "This represents the first time a broad-spectrum synbiotic has been clinically shown to increase both Urolithin A and butyrate in humans," said Zain Kassam, M.D., M.P.H., Chief Medical Officer at Seed Health. "DS-01® is delivering measurable biological impact via the microbiome—driving the production of key metabolites long studied for their roles in gut barrier integrity, inflammation, and healthy aging. These findings expand the biological relevance of DS-01® and point to new applications of microbiome-targeted innovations in advancing systemic health." About Seed Health Seed Health is a microbiome science company pioneering innovations in probiotics and living medicines to impact human and planetary health. Founded to realize the potential of the microbiome, our platform enables the translation of breakthrough science across a portfolio targeting health outcomes from infancy to aging. Our consumer innovations are commercialized under Seed® —an award-winning, science-first brand known for clinically validated innovations in probiotics. Our pipeline encompasses both indication-specific and preventive applications for gastrointestinal and digestive health, women's health, skin, pediatrics, mental health, metabolic function, and nutrition. Our environmental research is conducted under SeedLabs, which was founded to advance novel bacterial interventions to enhance biodiversity and restore ecosystems impacted by human activity. Press Contact [email protected] SOURCE Seed Health WANT YOUR COMPANY'S NEWS FEATURED ON PRNEWSWIRE.COM? 440k+ Newsrooms & Influencers 9k+ Digital Media Outlets 270k+ Journalists Opted In GET STARTED

NEW YORK--( BUSINESS WIRE )--Bonerge announced the initiation of its clinical trial, focusing on Fisetin, Urolithin A, and Ergothioneine. Involving 108 participants, this trial aims to evaluate their combined effects on skin health and organ rejuvenation when taking orally, providing more reliable options for the market. Senolytics Research: Advancing the Science of Anti-Aging In recent years, Senolytics research has advanced rapidly, with institutions like the Mayo Clinic and Wake Forest University at the forefront. Despite fruitful research, identifying optimal applications to translate Senolytics into market solutions remains crucial. In October 2024, the Mayo Clinic published groundbreaking findings in Nature Aging, identifying 67 plasma proteins, with IL-23R emerging as a promising anti-aging biomarker 1 . The study explored various promising Senolytics, including Fisetin, demonstrating a positive response in reducing senescence-related proteins and indicating potential for organ rejuvenation via senescent cell clearance 2 . The concept of organ rejuvenation offering deeper insights into its systemic effects and guiding anti-aging strategies. Senolytics show strong potential for organ rejuvenation applications and promise broader future prospects. Bonerge’s Clinical Trial: Exploring Skin as the Key to Rejuvenation The skin, the largest organ of the human body, is the most visible indicator of aging and a key target for anti-aging. Bonerge has launched a clinical trial focused on skin anti-aging, involving 108 participants. The trial evaluates skin anti-aging effects by assessing factors like skin heme, skin inflammation, transepidermal water loss, wrinkles, skin elasticity, skin firmness, melanin levels, and skin tone. Spanning 56 days, it aligns with two skin regeneration cycles, providing a detailed analysis of these key skin health indicators. Three ingredients tested in the trail: Fisetin: Among natural ingredients, fisetin has the strongest activity in clearing senescent cells. Its core mechanism promotes apoptosis of senescent cells and reduces SASP (Senescence-Associated Secretory Phenotype) secretion, alleviating chronic inflammation and combating inflammaging 3 , contributing to organ rejuvenation. Urolithin A: A rising star in nutritional supplements, Urolithin A has shown significant anti-photoaging effects. Photoaging, primarily caused by UVA (ultraviolet A) radiation, accelerates skin aging. Urolithin A helps protect skin by reducing the senescent phenotype of skin fibroblasts triggered by UVA, lowering intracellular ROS (reactive oxygen species), and activating antioxidant enzymes. These actions collectively support skin health and combat the harmful effects of photoaging 4 . Ergothioneine : Currently recognized as the only antioxidant that targets mitochondria, it reduces oxidative damage within mitochondria, supporting energy production and potentially preventing cellular aging 5 . Key Focus: Exploring Individual Ingredients and Combined Synergies In this clinical trial, while examining the skin anti-aging effects of individual ingredients, the combination of the three ingredients have also been tested to explore the synergistic effects on skin health and anti-aging. Path Ahead: Trial Progress and Future Expectations The clinical trial is progressing as planned and is expected to conclude in March 2025. Bonerge is hopeful that this study will provide valuable evidence for the anti-aging market, paving the way for the application of Senolytics in skin health and broader anti-aging treatments. References [1] Chase M. Carver, et al. IL-23R is a senescence-linked circulating and tissue biomarker of aging. Nature Aging, Dec 2024. [2] Researchers discover an aging and inflammation biomarker - Mayo Clinic News Network [3] Matthew J. et al. Fisetin is a senotherapeutic that extends health and lifespan. EBioMedicine 36 (2018). [4] Wenjie Liu , et al. Urolithin A protects human dermal fibroblasts from UVA-induced photoaging through NRF2 activation and mitophagy. J Photochem Photobiol B. 2022 Jul. [5] Brown, Lisa, et al. L-Ergothioneine and Its Protective Role in Cellular Health. Journal of Clinical Nutrition, vol 56.