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Last update 20 Mar 2025

Monoclonal antibody EP-5C7 (PDL BioPharma, Inc.)

Last update 20 Mar 2025

Overview

R&D Status

Clinical Result

Translational Medicine

Overview

Basic Info

Drug Type

Monoclonal antibody

Synonyms

Anti-E/P-selectin monoclonal antibody, Monoclonal antibody 5C7.29, Monoclonal antibody EP-5C7

+ [5]

Target

E-sel x P-sel

Action

inhibitors

Mechanism

E-sel inhibitors(Selectin E inhibitors), P-sel inhibitors(P-selectin inhibitors)

Therapeutic Areas

Immune System DiseasesNervous System DiseasesCardiovascular Diseases+ [3]

Active Indication-

Inactive Indication

AsthmaPsoriasisShock+ [1]

Originator Organization

PDL BioPharma, Inc.

Active Organization-

Inactive Organization

PDL BioPharma, Inc.

Drug Highest PhaseDiscontinuedPhase 1

First Approval Date-

Regulation-

100 Clinical Results associated with Monoclonal antibody EP-5C7 (PDL BioPharma, Inc.)

100 Translational Medicine associated with Monoclonal antibody EP-5C7 (PDL BioPharma, Inc.)

100 Patents (Medical) associated with Monoclonal antibody EP-5C7 (PDL BioPharma, Inc.)

Literatures (Medical) associated with Monoclonal antibody EP-5C7 (PDL BioPharma, Inc.)

01 Dec 2020·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

Generation and Characterization of Monoclonal Antibody Against Porcine Epidemic Diarrhea Virus Nonstructural Protein 13

Article

Author: Ding, Tong ; Wu, Shengqiang ; Ding, Zhen ; Tang, Yuxin ; Xie, Wenqing ; Chen, Jun ; Li, Ting ; Liang, Wenqi ; Ding, Nengshui

Porcine epidemic diarrhea virus (PEDV) is an enteric swine coronavirus. Recent PEDV eruption in East Asian and North American countries made it notorious and caused huge economic losses to the porcine industry. Nonstructural protein 13 (nsp13) is a nucleic acid helicase/NTPase that plays a critical role in viral gene transcription and viral replication. To investigate the function of nsp13 in the context of PEDV infection, here, PEDV nsp13 gene was amplified and cloned into pET28a/pET30a/pGEX-6P-1 expression vectors. The recombinant his-tagged nsp13 and GST-tagged nsp13 were expressed. Purified his-tagged nsp13 from pET28a-nsp13 vectors was chosen for immunization. Three monoclonal antibodies (mAbs) named 5A9, 5C7, and 5G7 were identified from the hybridoma cells, and were characterized by Western blot analysis and immunofluorescent assay, which demonstrated high specificity of these three mAbs with pCAGGS-HA-nsp13. All three mAbs belong to IgG1+ kappa subclass. However, only mAb 5A9 could effectively and specifically recognize PEDV expressing nsp13. Furthermore, the generated antibody against nsp13 could be applied to investigate nsp13 function during PEDV replication.

01 Dec 2020·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

Monoclonal Antibody: 5A9, 5C7, and 5G7 Against Porcine Epidemic Diarrhea Virus nsp13

Article

Author: Zhen Ding

Epidemic diarrhea virus nsp13 gene was amplified from PEDV (KX058033.1) and cloned into pET-28a expression vector.The solubilized and refolded inclusion bodies of his-tagged nsp13 from pET-28a-PEDV nsp13 vectors were chosen for immunization.One hundred micrograms of the recombinant nsp13 mixed with Freund′s adjuvant was s.c. immunized into 5-wk-old female BALB/c mice at 2-wk intervals.The procedure included three addnl. immunizations followed by a final booster injection.Pos. hybridoma cells were screened by using ELISA and subcloned three times by limiting dilutionThen, the stable hybridoma clones were obtained and injected into liquid paraffin-pretreated abdominal cavities of BALB/c mice to obtain ascites (mAbs).PCAGGS-HA-nsp13 vectors transfected into HEK 293T cells grown on microscope coverslips in 24-well plates.At 28 h post transfection, cells were inoculated with primary antibody at a dilution of 1:300 for 1 h at 37°C.After 3 times washing with PBST, cells were inoculated with fluorescein isothiocyanate isomer-labeled goat anti-mouse secondary antibody at a dilution of 1:200 at 37°C for 1 h.Images were acquired after cells were observed under the fluorescence microscope (Carl Zeiss).The results of immunofluorescent assay showed that all mAbs could bind to HA-tagged nsp13 and revealed specific green fluorescence, whereas neg. control cells could not reveal any fluorescence.

01 Aug 2010·Hybridoma

Synthetic Peptide Coupled to KLH Elicits Antibodies Against β8 Integrin

Article

Author: Zhang, Chenyang ; Liu, Yanjun ; Liao, Tingting ; Zhang, Chongjie ; Wei, Dapeng ; Luo, Zhijuan

The beta8 integrin, which forms alphavbeta8 heterodimers, is being widely investigated because of its specific structure and functions compared with other integrins. In this report, a 12 aa-long peptide of beta8 integrin cytoplasmic domain was synthesized according to a published sequence and covalently coupled to keyhole limpet hemocyanin (KLH). Three stable strains of hybridomas (3G6, 5C7, 5H3) that can secrete high specific monoclonal antibodies against beta8 integrin were successfully established by hybridoma technique. The isotypes of these MAbs were tested to be IgG2a. Their characterizations were shown by enzyme-linked immunosorbent assay (ELISA), Western blot analysis, and immunocytochemistry (ICC). The affinity constants (Kaff ) of the MAbs 3G6, 5C7, and 5H3 were measured by non-competitive ELISA respectively. Western blot analyses and immunocytochemistry demonstrated that all the MAbs were directed against beta8 integrin with high specificity.

100 Deals associated with Monoclonal antibody EP-5C7 (PDL BioPharma, Inc.)

R&D Status

10 top R&D records.

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Indication	Highest Phase	Country/Location	Organization	Date
Asthma	Phase 1	United States	PDL BioPharma, Inc.	-
Psoriasis	Phase 1	United States	PDL BioPharma, Inc.	-
Shock	Phase 1	United States	PDL BioPharma, Inc.	-
Stroke	Phase 1	United States	PDL BioPharma, Inc.	-

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Monoclonal antibody EP-5C7 (PDL BioPharma, Inc.)

Overview

Basic Info

Related

R&D Status

Clinical Result

Translational Medicine

Deal

Core Patent

Clinical Trial

Approval

Biosimilar

Regulation