Flosatidil

An HPLC method has been developed for the determination of SL 85.1016 (I), a new calcium antagonist arylbenzylamide methylthioether derivativeSL 85.1016 and the internal standard, SL 87.0210, are extracted from alk. human plasma with n-hexane and back extracted into 0.05 M phosphate buffer (pH 2.5; 0.2 mL).Acetonitrile (50 μL) is added to the final aqueous extract in order to prevent absorption of the compounds of interest on to the walls of the glass tube; this solution then is partially processed by HPLC on a C₁₈ column with UV detection (254 nm).The determination limit of the method is 2 ng.mL^-1 of I in human plasma; the response to the drug is linear in the range 2-200 ng.mg^-1.