Delving into the Latest Updates on ABY-029 with Synapse

Overview

Basic Info

Drug Type

Antibody-photosensitizer conjugates

Synonyms

ABY-029 trifluoroacetate salt

Target

EGFR

Mechanism

EGFR antagonists(Epidermal growth factor receptor erbB1 antagonists), Diagnostic dye

Therapeutic Areas

NeoplasmsNervous System Diseases

Active Indication

Glioma

Inactive Indication

Head and Neck NeoplasmsRecurrent GliomaSoft Tissue Sarcoma

Originator Organization

Dartmouth College

Active Organization

Affibody ABLi-Cor Bioscience

Dartmouth College

Inactive Organization

National Cancer Institute

Drug Highest PhasePhase 1

First Approval Date-

Regulation-

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Structure/Sequence

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The primary study objective is to determine if microdoses of ABY-029 (up to 6X) lead to detectable signals (defined as signal-to-noise ratio, SNR ≥10, with wide-field iFI) in sampled tissues with an EGFR (epidermal growth factor receptor) pathology score ≥ 1 based on histological staining.
The secondary study objective is to assess ex vivo the specificity of tumor binding in resected specimens by measuring the corresponding molecular uptake and concentrations using histopathology.

Start Date24 Jan 2018

Sponsor / Collaborator

Dartmouth-Hitchcock Medical Center

[+1]

NCT03154411

/ CompletedEarly Phase 1IIT

A Phase 0 Open Label, Single-center Clinical Trial of ABY-029, an Anti-EGFR Fluorescence Imaging Agent Via Single Intravenous Injection to Subjects With Primary Sarcoma.

The primary study objective is to determine if microdoses of ABY-029 (up to 6X) lead to detectable signals (defined as signal-to-noise ratio, SNR ≥10, with the Odyssey NIR scanner in sampled tissues with an EGFR pathology score ≥ 1 based on histological staining and compare SNR to tissues with an EGFR pathology score < 1).
The secondary study objective is to assess if the spatial patterns of EGFR expression correlate with the tumor targeting of ABY-029 detection by NIR scanner relative to histopathology diagnosis, and other indicators (e.g. proliferation, infiltration, etc.) as the gold standard, and to measure the molecular uptake and concentration of ABY-029 in resected specimens.

Start Date30 Aug 2017

Sponsor / Collaborator

Dartmouth-Hitchcock Medical Center

[+1]

NCT02901925

/ CompletedEarly Phase 1IIT

A Phase 0 Open Label, Single-center Clinical Trial of ABY-029, an Anti-EGFR Fluorescence Imaging Agent Via Single Intravenous Injection to Subjects With Recurrent Glioma

The primary study objective is to determine if microdoses of ABY-029 lead to detectable signals in sampled tissues with an EGFR pathology score ≥ 1 based on histological staining.
The secondary study objective is to assess diagnostic accuracy of ABY-029 detection by iFI and intraoperative probe relative to histopathology diagnosis, and other indicators (e.g. proliferation, infiltration, etc.) as the gold standard, and to measure the molecular uptake and concentration of ABY-029 in resected specimens.

Start Date13 Feb 2017

Sponsor / Collaborator

Dartmouth-Hitchcock Medical Center

[+2]

100 Clinical Results associated with ABY-029

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100 Translational Medicine associated with ABY-029

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100 Patents (Medical) associated with ABY-029

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26

Literatures (Medical) associated with ABY-029

01 Jun 2024·Annals of Biomedical Engineering

Quantifying Imaging Agent Binding and Dissociation in 3-D Cancer Spheroid Tissue Culture Using Paired-Agent Principles

Article

Author: He, Yusheng ; Brankov, Jovan G ; Tichauer, Kenneth M ; Xu, Xiaochun ; Torres, Veronica C ; Li, Chengyue ; Samkoe, Kimberley S ; Papavasiliou, Georgia ; Rounds, Cody C

Binding kinetics play an important role in cancer diagnosis and therapeutics. However, current methods of quantifying binding kinetics fail to consider the three-dimensional environment that drugs and imaging agents experience in biological tissue. In response, a methodology to assay agent binding and dissociation in 3-D tissue culture was developed using paired-agent molecular imaging principles. To test the methodology, the uptakes of ABY-029 (an IRDye 800CW-labeled epidermal growth factor receptor (EGFR)-targeted antibody mimetic) and IRDye-700DX carboxylate in 3-D spheroids were measured in four different human cancer cell lines throughout staining and rinsing. A compartment model (optimized for the application) was then fit to the kinetic curves of both imaging agents to estimate binding and dissociation rate constants of the EGFR-targeted ABY-029 agent. A statistically significant correlation was observed between apparent association rate constant (k₃) and the receptor concentration experimentally and in simulations (r = 0.99, p < 0.05). A statistically significant difference was found between effective k₃ (apparent rate constant of ABY-029 binding to EGFR) values for cell lines with varying levels of EGFR expression (p < 0.05), with no significant difference found between cell lines and controls for other fit parameters. Additionally, a similar binding affinity profile compared to a gold standard method was determined by this model. This low-cost methodology to quantify imaging agent or drug binding affinity in clinically relevant 3-D tumor spheroid models can be used to guide timing of imaging in molecular guided surgery and could have implications in drug development.

01 Apr 2024·Molecular Imaging and Biology

Neoadjuvant Therapies Do Not Reduce Epidermal Growth Factor Receptor (EGFR) Expression or EGFR-Targeted Fluorescence in a Murine Model of Soft-Tissue Sarcomas

Article

Author: Hoopes, P Jack ; Jarvis, Lesley A ; Werth, Paul M ; Pogue, Brian W ; Samkoe, Kimberley S ; Xu, Xiaochun ; Henderson, Eric R ; Paulsen, Keith D ; Hebert, Kendra A ; Streeter, Samuel S

PURPOSEABY-029, an epidermal growth factor receptor (EGFR)-targeted, synthetic Affibody peptide labeled with a near-infrared fluorophore, is under investigation for fluorescence-guided surgery of sarcomas. To date, studies using ABY-029 have occurred in tumors naïve to chemotherapy (CTx) and radiation therapy (RTx), although these neoadjuvant therapies are frequently used for sarcoma treatment in humans. The goal of this study was to evaluate the impact of CTx and RTx on tumor EGFR expression and ABY-029 fluorescence of human soft-tissue sarcoma xenografts in a murine model.PROCEDURESImmunodeficient mice (n = 98) were divided into five sarcoma xenograft groups and three treatment groups - CTx only, RTx only, and CTx followed by RTx, plus controls. Four hours post-injection of ABY-029, animals were sacrificed followed by immediate fluorescence imaging of ex vivo adipose, muscle, nerve, and tumor tissues. Histological hematoxylin and eosin staining confirmed tumor type, and immunohistochemistry staining determined EGFR, cluster of differentiation 31 (CD31), and smooth muscle actin (SMA) expression levels. Correlation analysis (Pearson's correlation coefficients, r) and linear regression (unstandardized coefficient estimates, B) were used to determine statistical relationships in molecular expression and tissue fluorescence between xenografts and treatment groups.RESULTSNeoadjuvant therapies had no broad impact on EGFR expression (|B|≤ 7.0, p ≥ 0.4) or on mean tissue fluorescence (any tissue type, (|B|≤ 2329.0, p ≥ 0.1). Mean tumor fluorescence was significantly related to EGFR expression (r = 0.26, p = 0.01), as expected.CONCLUSIONResults suggest that ABY-029 as an EGFR-targeted, fluorescent probe is not negatively impacted by neoadjuvant soft-tissue sarcoma therapies, although validation in humans is required.

01 Mar 2024·Biomedical Optics Express

Quantitative pharmacokinetic and biodistribution studies for fluorescent imaging agents

Article

Author: Samkoe, Kimberley S ; Hodge, Sassan ; Rounds, Cody ; Feng, Yichen ; Pannem, Sanjana ; Paulsen, Keith D ; Tichauer, Kenneth M

Pharmacokinetics and biodistribution studies are essential for characterizing fluorescent agents in vivo. However, few simple methods based on fluorescence imaging are available that account for tissue optical properties and sample volume differences. We describe a method for simultaneously quantifying mean fluorescence intensity of whole blood and homogenized tissues in glass capillary tubes for two fluorescent agents, ABY-029 and IRDye 680LT, using wide-field imaging and tissue-specific calibration curves. All calibration curves demonstrated a high degree of linearity with mean R2 = 0.99 ± 0.01 and RMSE = 0.12 ± 0.04. However, differences between linear regressions indicate that tissue-specific calibration curves are required for accurate concentration recovery. The lower limit of quantification (LLOQ) for all samples tested was determined to be < 0.3 nM for ABY-029 and < 0.4 nM for IRDye 680LT.

100 Deals associated with ABY-029

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R&D Status

10 top R&D records.

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Indication	Highest Phase	Country/Location	Organization	Date
Glioma	Phase 1	-	Affibody AB	-
Glioma	Phase 1	-	Dartmouth College	-
Glioma	Phase 1	-	Li-Cor Bioscience	-
Head and Neck Neoplasms	Discovery	US	Dartmouth College	24 Jan 2018
Soft Tissue Sarcoma	Discovery	US	Dartmouth College	30 Aug 2017
Recurrent Glioma	Discovery	US	National Cancer Institute	13 Feb 2017
Recurrent Glioma	Discovery	US	Dartmouth College	13 Feb 2017

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Clinical Result

Indication

Phase

Evaluation

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Study	Phase	Population	Analyzed Enrollment	Group	Results	Evaluation	Publication Date


NCT02901925 (ABY-029, CTgov)	Early Phase 1	Recurrent Glioma	14	ABY-029 (ABY-029 1X Dose Group)	tdyfvhnuoa(qhufrcmpbs) = cpmxmuejmg gkgeuvnnnf (ootdyuzlma, pyrgjuptxv - kdkufzxzfa) View more	-	13 Dec 2024
				ABY-029 (ABY-029 3X Dose Group)	tdyfvhnuoa(qhufrcmpbs) = nkngnrfeau gkgeuvnnnf (ootdyuzlma, kgwuywlpdm - tekpdvrboi) View more