NH-001

The outrageous development of modernization has greatly improved the life expectancy of human beings, but aging severely affects the quality of life, which is an urgent clin. challenge that needs to be addressed.As a common medicinal herb/health food for both medicine and foodstuff, jujube has attracted extensive attention due to its effects on improving sleep quality and prolonging life.Unfortunately, the chem. and biol. characteristics of jujube have not been fully characterized, it is necessary to systematically characterize the component profile and metabolic background of jujube, and the preliminary regulatory mechanism of jujube to exert anti-aging effect was further explored.Herein, we proposed a component characterization combined with metabolite profiling based on mass spectrometry (VIP ≥ 1, p < 0.05, FC ≥ 1.5 or FC ≤ 1/1.5) for identification, quality assessment and functional anal. of Jujube.The results suggested that in 12 batches of jujube samples from 3 regions of China, 43 differential components were identified to mediate the dynamic changes of 1571 endogenous metabolites, and then regulate 4 main pathways which are ascorbate and aldarate metabolism, sphingolipid metabolism, pyruvate metabolism and energy metabolism, to play a high edible and medicinal value of jujube.Subsequently, we employed mol. docking technol. in conjunction with microscale thermophoresis affinity detection (60 % LED excitation power and 40 % MST power in NT. Control) to measure the binding affinities of differential components to specific targets.As anticipated, unique constituents in jujube, including glabrolide, betulinic acid, magnoflorine, and cytochalasin B, exhibited significant binding affinities to target proteins associated with the regulation of immune responses in the body.This study further revealed the potential regulatory mechanism of long-term consumption of jujube to "prolong life and improve health", and provided a theor. basis for the quality control, classification processing and functional research of jujube.

Dopaminergic system gains importance in homeostatic sleep regulation, but the role of different dopamine receptors is not well-defined. 72 h rat electrocorticogram and sleep recordings were made after single application of dopaminergic drugs in clinical use or at least underwent clinical trials. The non-selective agonist apomorphine evoked short pharmacological sleep deprivation with intense wakefulness followed by pronounced sleep rebound. D2 agonist bromocriptine induced moderate and extended increase in wakefulness without a homeostatic sleep replacement but downregulated slow wave sleep need for 72 h. Selective D1 agonist SKF-38393 failed to induce enhanced waking sufficient for sleep replacement. High-dose D2 antagonism by sulpiride temporarily enhanced wakefulness. All drugs evoked extended (72 h) sleep changes after single application. Opposite sleep changes could be seen after the application of different doses in case of both bromocriptine and sulpiride. Theta, beta and gamma power reflected intensity differences in drug-induced wakefulness stages. Apomorphine- and high sulpiride dose-induced waking showed elevated power in all three frequency bands. Bromocriptine-induced wakefulness dominated by beta activity. Enhancement of more, than one type of electrocorticogram activities during wakefulness was a prerequisite for the activation of sleep homeostasis. According to present data, D1- or D2-like receptor agonism are not separately involved in the homeostatic regulation of slow wave sleep. Simultaneous and non-selective agonism on DA receptors is the most effective way to elicit intense W, which is followed by slow wave sleep rebound. REM sleep rebound could be evoked by D2 agonism. Rebound indicates the activation of homeostatic sleep regulation, but with unknown exact mechanisms.