L-tyrosine

Transmissible gastroenteritis virus (TGEV) infection induces diarrhea in piglets by targeting the small intestine, especially the jejunum and ileum. However, dynamic changes in the gut microbiota and metabolome during TGEV infection remain unclear. This study investigated these alterations and their association with intestinal damage in weaned pigs during early TGEV infection. Thirty 4-week-old pigs were allocated randomly into TGEV-inoculated and mock groups. On days 3, 5, and 7 postinoculation, intestinal tissue and fecal samples were collected. Full-length 16 S rRNA sequencing and ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC‒MS/MS) were employed to analyze microbiota composition and nontargeted metabolites. TGEV infection resulted in reduced villous height-to-crypt depth (VH:CD) ratios (P < 0.01) and significantly altered microbial diversity (P = 0.0091 in jejunum) and composition (P = 0.001). Notably, infected pigs showed increased abundances of Lactobacillus and Limosilactobacillus species. The VH:CD ratio correlated with the overall taxonomic composition in both the jejunum and ileum (r = 0.4, P < 0.001) and was positively associated with microbial functions such as aerobic chemoheterotrophy and chitinolysis in the jejunum. Fecal metabolomics revealed 1815 and 892 differentially expressed metabolites in the jejunum and ileum, respectively, including amino acids, fatty acids, and intermediates of energy metabolism. Integrated analysis revealed that Lactobacillus amylovorus DSM20531 was positively correlated with linoleic acid, L-tyrosine, and citric acid, whereas Lactococcus lactis showed a negative correlation with isocitric acid and glutamine. This study enhances our understanding of the pathogenesis of TGEV and provides potential microbial and metabolic biomarkers for future diagnostic and preventive strategies.

This study systematically investigated the genetic and metabolic mechanisms underlying pigmentation in goose webbed feet by integrating histological, transcriptomic, and metabolomic analyses. Histological examinations revealed significant differences in melanin deposition among webbed feet of varying colors. Dark black webbed feet exhibited the highest melanin content, light black webbed feet showed moderate levels, and colorless webbed feet lacked detectable melanin. Transcriptomic analysis identified substantial variations in the expression levels of key genes involved in melanin biosynthesis, including TYRP1, PMEL, DCT, TYR, OCA2, MC1R, RAB38, WNT16, CAMK2A, and MLANA, between pigmented and colorless webbed feet. Notably, the OCA2 gene exhibited significantly higher expression in dark black webbed feet compared to light black webbed feet, underscoring its pivotal role in regulating pigmentation intensity. Enrichment analysis emphasized the importance of pathways related to tyrosine metabolism, melanin production, and amino acid biosynthesis in determining pigmentation differences. Metabolomic profiling supported these findings, revealing that L-tyrosine and 5,6-dihydroxyindole-2-carboxylic acid are critical metabolites in the melanin biosynthesis pathway. Specifically, elevated levels of L-tyrosine were detected in colorless webbed feet, likely due to inhibited melanin synthesis, whereas 5,6-dihydroxyindole-2-carboxylic acid levels were highest in dark black webbed feet, reflecting active melanin production. Correlation analysis between transcriptomic and metabolomic data further validated the central role of tyrosine metabolism and melanin biosynthesis pathways in pigmentation. In conclusion, this study employed multi-omics approaches to elucidate the critical role of the OCA2-centered genetic-metabolic regulatory network in melanin deposition of goose webbed feet, providing important insights into the molecular mechanisms of avian pigmentation and valuable references for poultry breeding.