CAMBRIDGE, Mass., Oct. 11, 2022 /PRNewswire/ -- GentiBio, Inc., a biotherapeutics company developing engineered T regulatory (Treg) cells (EngTregs) therapies for autoimmune, auto-inflammatory, alloimmune and allergic diseases, today announced the release of preclinical data demonstrating a novel approach for using engineered Tregs to potentially treat and prevent type 1 diabetes (T1D). GentiBio and colleagues demonstrated the ability of EngTregs to specifically target pancreas cells and draining lymph nodes in order to mitigate destruction and inflammation associated with the development of T1D within the context of ongoing inflammation. These data were released both by publication and presentation in collaboration with colleagues at the Seattle Children's Research Institute and Benaroya Research Institute. GentiBio will present their study today in a poster presentation at the European Society of Gene and Cell Therapy (ESGCT) Congress in Edinburgh, Scotland. The poster is entitled "Development and characterization of GNTI-122, an autologous engineered human regulatory T cell therapy for type 1 diabetes." Additional data were also published last week in the peer reviewed journal, Science Translational Medicine, in a paper entitled "Pancreatic islet-specific engineered Tregs exhibit robust antigen-specific and bystander immune suppression in type 1 diabetes models." T1D, also known as juvenile diabetes, is an autoimmune disease caused by the immune system destroying the cells of the pancreas responsible for making insulin. The data in the paper and publication demonstrated that, in a T1D mouse model, GentiBio's approach specifically suppressed pathogenic T cells attacking the pancreas to reduce inflammation, protect insulin-producing islet beta cells, and mitigate the development of T1D. Furthermore, the data demonstrated that human EngTregs could be effectively engineered and produced at high purity with GentiBio's unique technology that overcomes key challenges associated with developing Tregs to treat autoimmune disease. "We are incredibly excited about the potential for GNTI-122 to dramatically transform the lives for patients with type 1 diabetes," stated Tom Wickham, PhD, chief scientific officer. "The data from GentiBio and our collaborators at Seattle Children's Research Institute and Benaroya Research Institute support the ability of our unique technology to address key bottlenecks associated with developing a Treg therapeutic by providing a highly potent, stable, and scalable EngTreg to treat T1D." The company's approach involves creating differentiated, engineered Tregs that integrate all three of the company's technology pillars outlined below:
Human CD4+ cells from the patient are engineered to stably express FOXP3 to create and retain a robust and highly suppressive Treg phenotype, even within the context of inflammation, overstimulation, or deprivation of IL-2 which are often present in autoimmune disease. lL-2 signaling support, via DuraReg™, is engineered into the EngTregs to overcome demonstrated scarcity of IL-2 in T1D and many other autoimmune diseases that is essential for Treg survival, expansion, and function.
A highly potent T cell receptor (TCR) is incorporated to provide maximal suppressive activity against T cells attacking the islet cells within the pancreas and guide EngTregs trafficking to draining lymph nodes. A novel TCR screening and qualification pipeline is utilized to isolate and identify the rare TCRs optimal for pairing with EngTregs, while eliminating the endogenous TCR. A novel, highly scalable, dual-editing method has been developed which enables all three pillars to be incorporated into a highly enriched population of EngTregs that can be manufactured and delivered to a patient.
"We believe our approach to isolate and identify highly specific TCRs that function optimally within EngTregs for application to T1D could signal our ability to develop important cell therapies for many other allergic, inflammation and autoimmune disorders," stated Jane Buckner, MD, president of Benaroya Research Institute. "We hope that someday this therapy could become a groundbreaking treatment."