Ultrasonic wave auxiliary method was used to withdraw the Lycopene in the watermelon, selected 5 kind of factors to carry on the single factor experiment first, compared the withdraw effect with the single factor, the factor with the highest withdraw effect was selected to perform the orthogonal test. The test result indicated that the primary factor affected the Lycopene extraction was the extraction temperature, the extraction time, the supersonic power and the chem. additive. In the temperature of 45 degree C, under the supersonic power of 140 W, ratio of material to liquid of 1: 3, added 0.5% sucrose solution, withdrew 6 min, the effect was best.