The white rot fungi Pleurotus florida was obtained and stored at 4°C.The enzyme laccase was produced on basal media and the enzyme activity and protein content was estimatedDifferent substrate (Saw dust, coir pith and straw) was used for the maximum laccase productionThe enzyme characterization was done by studying the effect of pH and temperature on laccase activity, and the optimum day for laccase production were also calculatedFor the induction of enzyme production, copper was used in different concentration, among these 150 μM CuSo4 induced maximum laccase production