Jiangxi Huiren Pharmaceutical Co., Ltd.

The aim of this study was to elucidate the mechanism of action of Shenbao tablets using metabolomics approach.

Kidney-Yang deficiency is a common syndrome type in traditional Chinese Medicine (TCM) syndrome typology, closely related to disorders of multiple metabolic pathways and is the root cause and underlying syndrome type of many diseases. Shenbao tablets can significantly improve the main symptoms of kidney yang deficiency syndrome, but the mechanism of action of Shenbao tablets on kidney yang deficiency syndrome is still unknown.

The rats were intraperitoneally injected with hydrocortisone once a day for 40 days to simulate the syndrome. Traditional pharmacodynamic indicators (body mass, biochemical indicators and pathology) were used to evaluate the efficacy of the medicine. Serum, urine and feces were collected from rats. UPLC/MS metabolomics method was used to study the overall metabolic profile of serum, while GC/MS metabolomics method was used to study the metabolic spectrum of urine and feces.

Results showed that the syndrome was significantly improved in the treatment group, and obvious metabolic disorders were observed in rats with the syndrome, with 47 potential biomarkers identified. Pathway analysis showed that nicotinate and nicotinamide metabolism, glycine, serine and trione metabolism, aminoacyl tRNA biosynthesis, glycoxylate and dicarboxylate metabolism were the major ways for Shenbao tablet to improve kidney-yang deficiency syndrome.

The mechanism of action of Shenbao tablet in improving the syndrome involves the regulation of energy metabolism, amino acid metabolism, bile acid metabolism, fatty acid metabolism and intestinal microorganisms. This work shows that metabolomics is a promising tool for studying the essence of syndrome theory in TCM and the mechanisms of TCM.

The different steaming temperature was investigated by using modern industrialized production equipment.The traits and the content of catalpol, Rehmaionoside A, verbascoside, 5-hydroxymethylfurfural, glucose were used as indicators to obtain the best high-pressuresteaming processing technol. of prepared Radix Rehmanniae, then the established process was validated.The results showed that the best processing technol. of prepared Radix Rehmanniae was high-pressure steaming at 120 °C for 2 h and braising statically for 1 h.The process was stable and suitable for industrial production, and could ensure the quality of prepared Radix Rehmanniae.

Baicalin content during extraction and concentration of Nujin capsules were on line analyzed with sound and light adjustable filter-near IR spectroscopy.The extraction and concentration samples were on line collected, data of baicalin content was established, near IR spectra were collected at the same time, the correction model between near IR spectra and data of baicalin content was established with partial least square method, and contents of the predictive samples collected on line were predicted to confirm the established model.The correlation coefficient R² of the correction model between near IR spectra and baicalin content data of reflux extract and concentrated solution was 0.9794 and 0.9855 resp., the prediction model correlation coefficient R² of the external samples was 0.9969 and 0.9844 resp., and the average relative deviation was 4.46% and 4.76% resp.The precision of the method was 1.93% and 1.86% resp., the 5 h stability RSD was 1.16% and 1.07% resp., and the predictive recovery was 99.3% and 99.3% resp.Near IR spectroscopy used in anal. of baicalin content during extraction and concentration of Nujin capsules is rapid and direct, which could realize site anal.