Aim and Methods: A coronavirus disease 2019 (COVID-19) cluster emerged in a manufacturing factory in early August 2021. In Nov. 2021, a ventilation survey using tracer gas and data anal. was performed to reproduce the situation at the time of cluster emergence and verify that ventilation in the office increased the risk of aerosol transmission; verify the effectiveness of measures implemented immediately in August; and verify the effectiveness of addnl. measures when previously enforced measures proved inadequate. Results: At the time of cluster emergence, the average ventilation frequency was 0.73 times/h, less than the 2 times/h recommended by the Ministry of Health, Labour, and Welfare; as such, the factory's situation was deemed to have increased the risk of aerosol transmission. Due to the measures already taken at the time of the survey, the ventilation frequency increased to 3.41 times/h on average It was confirmed that ventilation frequency increased to 8.33 times/h on average, when addnl. measures were taken. Conclusion: To prevent the re-emergence of COVID-19 clusters, it is necessary to continue the measures that have already been implemented. Addnl., introduction of real-time monitoring that visualizes CO2 concentrations is recommended. Furthermore, we believe it is helpful that external researchers in multiple fields and internal personnel in charge of health and safety department and occupational health work together to confirm the effectiveness of conducted measures, such as this case.