Fiber d. can be used to estimate composite weight and to evaluate fiber content for property predictions in fiber-reinforced composites.Yet, natural fibers don′t have fixed d. value given on datasheet and their d. measurement is affected by various factors.This study compares various standard natural fiber′s d. mearusing methods and identifies determinant factors to, then, estimate the d. of Enset fibers by using the optimal combination.The advantages and disadvantages of five standard methods were compared considering the characteristics of natural fibers′ and methods′ effectiveness where Helium gas Pycnometry is selected afterward.Standard calibration sphere and synthetic fiber with known d. is measured for validation.The study analyzed the impact of variables on result′s precision and accuracy.Then, D. of Enset fiber have been measured with varied factors including uniformity and duration purging, volume cell type and amount of fiber in a cell, and fiber size.The result showed that fluctuation is minimized, and accuracy and precision is enhanced for the larger volume cell, larger mass of fiber in a cell, smaller size fiber and longer uniform purging.Finally, measured d. of Enset using the best combination of factors is 1.38 ± 0.01 g/cm3.