In this single-center, open-label, no control, prospective clinical trial, a total of 20 resistant or refractory CD19+ B cell acute lymphoblastic leukemia (ALL) patients will be enrolled. CD19 CAR T cells will be administered by i.v. injection as a using a "split dose" (total dose of 5x10^6/kg-5x10^7/kg) approach to dosing:10% on day 0, 30% on day 1 and 60% on day 2. The purpose of current study is to determine the clinical efficacy and safety of CD19 CAR T cells in patients with chemotherapy resistant or refractory CD19+ ALL.

Start Date01 Nov 2016

Sponsor / Collaborator

Union Stem Cell & Gene Engineering Co., Ltd.

[+1]

100 Clinical Results associated with Union Stem Cell & Gene Engineering Co., Ltd.

0 Patents (Medical) associated with Union Stem Cell & Gene Engineering Co., Ltd.

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01 Apr 2024·Zhongguo shi yan xue ye xue za zhi

[The Optimal Storage Condition and Storage Time of Umbilical Cord Blood from Collection to Preparation].

Article

Author: Zhang, Ya-Bin ; He, Xue-Ping ; Yang, Jun-Ye ; Zhang, Yong ; Qiu, Lu-Gui ; Guo, Rui ; Yang, Wen-Ling ; Han, Jun-Ling

OBJECTIVETo explore the optimal storage condition and time of umbilical cord blood from collection to preparation.METHODSCollect cord blood samples from 30 healthy newborns, with each new born's umbilical cord blood was divided into two parts on average. One part was stored in cold storage (4 ℃) and the other was stored at room temperature (20-24 ℃). Samples were taken at 24, 36, 48, 60 and 72 h, respectively, total nucleated cells (TNC) count and TNC viability was analyzed. Flow cytometry was used to detect the ratio of viable CD34⁺ cells to viable CD45⁺ cells and viability of CD34⁺ cells, and colony-forming unit-granulocyte-macrophage (CFU-GM) count was performed by hematopoietic progenitor cell colony culture. The change trend of each index over time was observed, and the differences in each index was compared between cold storage and room temperature storage under the same storage time.RESULTSThe TNC count (r _{4 ℃}=-0.9588， r _{20-24 ℃}=-0.9790), TNC viability (r _{4 ℃}=-0.9941， r _{20-24 ℃}=-0.9970), CD34⁺ cells viability (r _{4 ℃}=-0.9932， r _{20-24 ℃}=-0.9828) of cord blood stored in cold storage (4 ℃) and room temperature storage (20-24 ℃) showed a consistent downward trend with the prolongation of storage time. The percentage of viable CD34⁺ cells (r _{4 ℃}=0.9169， r _{20-24 ℃}=0.7470) and CFU-GM count (r _{4 ℃}=-0.2537， r _{20-24 ℃}=-0.8098) did not show consistent trends. When the storage time was the same, the TNC count, TNC viability, CD34⁺ cells viability and CFU-GM count of cord blood stored in cold storage were higher than those stored at room temperature. Under the same storage time (24, 36, 48, 60 or 72 h), TNC viability in room temperature storage was significantly lower than that in cold storage (P <0.001), but TNC count, percentage of viable CD34⁺ cells and CFU-GM count were not significantly different between room temperature storage and cold storage. When stored at room temperature for 24 h and 36 h, the viability of CD34⁺ cells was significantly lower than that in cold storage (P <0.001, P <0.01), when the storage time for 48, 60 and 72 h, there was no significant difference in the CD34⁺ cells viability between room temperature storage and cold storage.CONCLUSIONIt is recommended that cord blood be stored in cold storage (4 ℃) from collection to preparation, and processed as soon as possible.

01 Dec 2022·Journal of NanobiotechnologyQ2 · ENGINEERING & TECHNOLOGY

The sustained PGE2 release matrix improves neovascularization and skeletal muscle regeneration in a hindlimb ischemia model

Q2 · ENGINEERING & TECHNOLOGY

ArticleOA

Author: Benkirane-Jessel, Nadia ; Zhang, Jun ; Li, Zongjin ; Chen, Shang ; Chang, Ying ; Cao, Jiasong ; Cheng, Hui ; Hua, Guoqiang ; Han, Zhong-Chao ; Shen, Xiaohong ; Du, Wei ; Chang, Yuqiao ; Huang, Haoyan ; Guo, Zhikun ; Han, Zhibo

AbstractBackgroundThe promising therapeutic strategy for the treatment of peripheral artery disease (PAD) is to restore blood supply and promote regeneration of skeletal muscle regeneration. Increasing evidence revealed that prostaglandin E2 (PGE2), a lipid signaling molecule, has significant therapeutic potential for tissue repair and regeneration. Though PGE2 has been well reported in tissue regeneration, the application of PGE2 is hampered by its short half-life in vivo and the lack of a viable system for sustained release of PGE2.ResultsIn this study, we designed and synthesized a new PGE2 release matrix by chemically bonding PGE2 to collagen. Our results revealed that the PGE2 matrix effectively extends the half-life of PGE2 in vitro and in vivo. Moreover, the PGE2 matrix markedly improved neovascularization by increasing angiogenesis, as confirmed by bioluminescence imaging (BLI). Furthermore, the PGE2 matrix exhibits superior therapeutic efficacy in the hindlimb ischemia model through the activation of MyoD1-mediated muscle stem cells, which is consistent with accelerated structural recovery of skeletal muscle, as evidenced by histological analysis.ConclusionsOur findings highlight the chemical bonding strategy of chemical bonding PGE2 to collagen for sustained release and may facilitate the development of PGE2-based therapies to significantly improve tissue regeneration.Graphical Abstract

01 Aug 2017·Oncotarget

A molecular pathology method for sequential fluorescence in situ hybridization for multi-gene analysis at the single-cell level

Article

Author: Hu, Linping ; Sun, Jiangman ; Zetterberg, Anders ; Yin, Xiuxiu ; Miao, Weimin ; Cheng, Tao

Multi-gene detection at the single-cell level is desirable to enable more precise genotyping of heterogeneous hematology and oncology samples. This study aimed to establish a single-cell multi-gene fluorescence in situ hybridization (FISH) method for use in molecular pathology analyses. Five fluorochromes were used to label different FISH gene probes, and 5 genes were detected using a five-color FISH protocol. After the first hybridization, the previous FISH probe set was stripped, and a second set of five-color FISH probes was used for rehybridization. After each hybridization, the fluorescence signals were recorded in 6 fluorescence filter channels that included DAPI, Spectrum Green^™, Cy3^™ v1, Texas Red, Cy5, and PF-415. A digital automatic relocation procedure was used to ensure that exactly the same microscopic field was studied in each stripping and hybridization cycle. By using this sequential stripping and rehybridization strategy, up to 20 genes can be detected within a single nucleus. In conclusion, a practical molecular pathology method was developed for analyzing multiple genes at the single-cell level.

100 Deals associated with Union Stem Cell & Gene Engineering Co., Ltd.

100 Translational Medicine associated with Union Stem Cell & Gene Engineering Co., Ltd.

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