Zhejiang Gongshang University

Whey protein-associated mouthdrying during oral processing is a driving factor for consumers′ dislike of high-protein beverages, however, remains mechanistically underexplored in dairy systems.This study investigated the fundamental mechanisms driving powderiness in whey protein-enriched dairy beverages (8 % weight/weight protein).We conducted a comparative anal. of whey protein concentrate (WPC) vs. whey protein isolate (WPI) at various casein-whey ratios (80:20 to 20:80) and evaluated thermal processing effects (63 °C/85 °C for 30 min) from rheol. characterization, particle size distribution, tribol. properties, and interfacial adsorption kinetics.Results demonstrated that protein composition significantly altered frictional behavior, with WPC-dominated systems (casein-whey protein ratio of 20:80) exhibiting 33.6 % higher friction coefficients vs. WPI counterparts at 10 mm/s without heating.Thermal treatment at 85 °C induced substantial aggregation in WPI-dominant systems (hydrodynamic diameter increased from 0.150 μm to 0.167 μm) and reduced adsorption capacity by 30.80 %, correlating with impaired lubrication.Conversely, WPC maintained structural homogeneity and enhanced interfacial coverage post-heating, preserving lubrication functionality.These findings establish composition-specific thermal processing guidelines for optimizing sensory attributes in high-protein dairy beverages.

The water pollution issue triggered by antibiotic was a great challenge facing humanity, and it was necessary to develop an effective remediation technique. In this work, Fe₂O₃/Co₃O₄ composite with internal electric field was fabricated by a simple method. The presence of internal electric field reduced the interfacial resistance and facilitated the charge transfer, so stimulating the electron transport during reaction process. With the inducement of electrostatic force based on internal electric field, two active areas (namely electron-rich region and electron-deficient region) were formed at Fe₂O₃/Co₃O₄ composite. The electron-deficient active area (namely Co₃O₄ component) can oxidize peroxymonosulfate (PMS) to produce SO₅^•-, further turning into ¹O₂. In the meantime, the Fe₂O₃ component as electron-rich active area provided electrons to achieve the Fe-O-O heterolysis, then generating high-valent metal complexes. As predicted, the Fe₂O₃/Co₃O₄-driven PMS system displayed excellent ability to remove ofloxacin. Furthermore, the micro reactor loaded with Fe₂O₃/Co₃O₄ composite exhibited satisfactory performance in treating the wastewater containing ofloxacin. All in all, the effects of internal electric field on PMS activation are investigated in depth, which provided a valuable reference for future research.

Rapid and sensitive nucleic acid detection is very important for the detection of Salmonella in food. Convenient detection methods show great potential in food safety. CRISPR-Cas12a system has been widely used for nucleic acid detection. In one-pot reaction, Cas12a can perform cis- and trans-cleavage of amplicons and primers, which reduces the sensitivity of the reaction and has certain limitations. In this study, we integrated the Cas12a cutting system with recombinase polymerase amplification (RPA) into one microfluidic chip to avoid cap opening pollution. A portable observation device was integrated to enable visual detection of signals with the naked eye. The detection technology could specifically detect 1 × 10° CFU/mL Salmonella in 50 min at 37 °C. In the field test, there was no need for professional equipment or high-tech means.