Ya'an Sanjiu Pharmaceutical Co., Ltd.

The efficiency of vacuum extraction and conventional extraction of Epimedium brevicornum was compared.Taking composite score of icariin and total flavonoids extraction rate as index, L₉ (3⁴) orthogonal test was used to optimized vacuum extraction and conventional extraction technol. of E. brevicornum, and extraction efficiency of them was compared.Extraction efficiency of vacuum extraction was significantly better than that of conventional extractionThe optimum vacuum extraction conditions were as followings: extracted 3 times with 10 times the amount of 60% ethanol at 50°C, 20 min each time, vacuum degree 0.07 MPa.The optimum conventional extraction conditions were: extracted 3 times with 10 times the amount of 60% ethanol, 1 h each time.Extraction effect of vacuum extraction 1 h and conventional extraction 3 h was equal, and yield of dry extract with vacuum extraction could be reduced by about 30% than that of conventional extractionVacuum extraction was superior to conventional extraction technol., and the former was effective and stable, it could be used for industrial production of E. brevicornum.

The objective was to optimize the process of extracting effective constituents from Radix Salvia by central composite design/response surface methodol.The effects of influence factors such as solvent fold, ethanol concentration and extract time on extraction rate of tanshinone II a and salvianolic acid were investigated by using box-behnken design and response surface method.The data were imitated using multi-linear equation and second-order polynomial equation.Multiple correlation coefficients from second-order polynomial equation were prior to those from multi-linear equation.The optimum condition of extraction process was 9 fold solvent, 1.5 h for decoction, and 3 times for extraction; and the ethanol concentration was 60%.Bias between observed and predicted values was less than 3%.The optimum model was highly predictive.

The optimization conditions for the formation of total flavonoids in herba Epimedium complex with phospholipid was chosen.Solvent evaporation process was used to prepare total flavonoids in herba Epimedium phospholipid complex.The evaluation criterion is complex ratio.The experiment inspect the significance of influential factor of preparation technol. by Plackett-Burman design.Optimized significance of factor and level with the Box-Behnken design.The binomial and linear equations were fitted to the data of overall desirabilities, and the resulting equation was used to produce 3-D response surface graphs, through which optimal formulation were predicted.Total flavonoids and phospholipid ratio, flavonoids concentration and reaction temperature had significance influence to the craft.It showed that the correlation coefficient of second-order quadratic model was high.Correlation coefficient reached 0.988.The optimum conditions for the preparation of total flavonoids in Epimedium on complex were as follows: phospholipids-total flavonoids being 3.6: 1, time 2.4 h, temperature at 40° and concentration of principal agent being 10 g/L.The deviation between result of the best craft verification test and the binomial fitting equation forecast value is less than 2.0%.Response surface methodol. was successfully used to optimize the preparation of total flavonoids in Herba Epimedium on phospholipid complex.Optimized process was reliable, stable and available for industrial production